LOCUS       sequence-320243-       11106 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX
FEATURES             Location/Qualifiers
     source          1..11106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2561..6661
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7961..7981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7990..8007
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8030..8047)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8036..8260
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8331..8347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8331..8347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8344..8366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8355..8371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8379..8385,8386..8403,8404..8409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8424..8445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8562..8579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8733..9321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8813..8832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9492..10283,10284..10352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10115..10134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10353..10457)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10536..10555)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10727..11106
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtcatggcc ggagcacagg attttgtccc
      361 acatactgcc gatctggctg agctggctgc cgccgctgga gagtgtagag gatgcgggct
      421 gtaccgggat gccacacagg ccgtgttcgg agcaggcggc aggagcgccc gcatcatgat
      481 gatcggcgag cagccaggcg ataaggagga cctggccggc ctgccatttg tgggaccagc
      541 aggccggctg ctggacagag ccctggaggc cgccgacatc gatagggacg ccctgtacgt
      601 gaccaacgcc gtgaagcact tcaagtttac acgcgccgca ggaggcaaga ggagaatcca
      661 caagaccccc tctcggacag aggtggtggc ctgcagacct tggctgatcg ccgagatgac
      721 cagcgtggag ccagatgtgg tggtgctgct gggagcaaca gcagcaaagg ccctgctggg
      781 caatgacttc agggtgaccc agcacagggg agaggtgctg cacgtggacg atgtgccagg
      841 cgatcctgcc ctggtggcaa ccgtgcaccc tagctccctg ctgagaggcc caaaggagga
      901 gcgggaatcc gccttcgctg ggctggtgga cgacctgaga gtggctgccg atgtgagacc
      961 ttctggagga tctagcggag gctcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     1021 aacaccagag agcagtggcg gcagcagcgg ggggtcatcc tcagagactg ggcctgtcgc
     1081 cgtcgatcca accctgcgcc gccggattga acctcacgag tttgaagtgt tctttgaccc
     1141 ccgggagctg agaaaggaga catgcctgct gtacgagatc aactggggag gcaggcactc
     1201 catctggagg cacacctctc agaacacaaa taagcacgtg gaggtgaact tcatcgagaa
     1261 gtttaccaca gagcggtact tctgccccaa taccagatgt agcatcacat ggtttctgag
     1321 ctggtcccct tgcggagagt gtagcagggc catcaccgag ttcctgtcca gatatccaca
     1381 cgtgacactg tttatctaca tcgccaggct gtatcaccac gcagacccag agaataggca
     1441 gggcctggag gatctgatca gctccggcgt gaccatccag atcatgacag agcaggagtc
     1501 cggctactgc tggcggaact tcgtgaatta ttctcctagc aacgaggccc actggcctag
     1561 gtacccacac ctgtgggtgc gcctgtacgt gctggagctg tattgcatca tcctgggcct
     1621 gcccccttgt ctgaatatcc tgcggagaaa gcagccccag ctgaccttct ttacaatcgc
     1681 cctgcagtct tgtcactatc agaggctgcc accccacatc ctgtgggcca caggcctgaa
     1741 gtctggagga tctagcggag gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     1801 aacaccagag agcagtggcg gcagcagcgg cggcagcatg gccggagcac aggattttgt
     1861 cccacatact gccgatctgg ctgagctggc tgccgccgct ggagagtgta gaggatgcgg
     1921 gctgtaccgg gatgccacac aggccgtgtt cggagcaggc ggcaggagcg cccgcatcat
     1981 gatgatcggc gagcagccag gcgataagga ggacctggcc ggcctgccat ttgtgggacc
     2041 agcaggccgg ctgctggaca gagccctgga ggccgccgac atcgataggg acgccctgta
     2101 cgtgaccaac gccgtgaagc acttcaagtt tacacgcgcc gcaggaggca agaggagaat
     2161 ccacaagacc ccctctcgga cagaggtggt ggcctgcaga ccttggctga tcgccgagat
     2221 gaccagcgtg gagccagatg tggtggtgct gctgggagca acagcagcaa aggccctgct
     2281 gggcaatgac ttcagggtga cccagcacag gggagaggtg ctgcacgtgg acgatgtgcc
     2341 aggcgatcct gccctggtgg caaccgtgca ccctagctcc ctgctgagag gcccaaagga
     2401 ggagcgggaa tccgccttcg ctgggctggt ggacgacctg agagtggctg ccgatgtgag
     2461 acctagcggt ggtagttctg gcggtagctc aggatctgaa actcccggca catctgaaag
     2521 cgcgactccc gaatcatctg gtggatcttc aggtggaagt gacaagaagt acagcatcgg
     2581 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     2641 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     2701 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     2761 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     2821 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     2881 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     2941 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     3001 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     3061 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3121 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     3181 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     3241 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     3301 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     3361 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     3421 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     3481 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     3541 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     3601 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     3661 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     3721 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     3781 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     3841 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     3901 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     3961 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     4021 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     4081 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     4141 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     4201 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     4261 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     4321 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     4381 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     4441 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     4501 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     4561 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     4621 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     4681 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     4741 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     4801 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     4861 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     4921 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     4981 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     5041 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     5101 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     5161 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     5221 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     5281 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     5341 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     5401 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     5461 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     5521 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     5581 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     5641 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     5701 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     5761 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     5821 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     5881 gtctatcagg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     5941 taagaagtac ggcggcttcg tcagccccac cgtggcctat tctgtgctgg tggtggccaa
     6001 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     6061 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     6121 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     6181 cggccggaag agaatgctgg cctctgccag attcctgcag aagggaaacg aactggccct
     6241 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     6301 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     6361 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     6421 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     6481 tatcatccac ctgtttaccc tgaccaatct gggagcccct agggccttca agtactttga
     6541 caccaccatc gaccggaagg tgtacaggag caccaaagag gtgctggacg ccaccctgat
     6601 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga
     6661 ctctggagga tctagcggag gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     6721 aacaccagag agcagtggcg gcagcagcgg cggcagcatg gttgaagcag atcgcccagg
     6781 aaagctcttc attggtgggc ttaatacgga aacaaatgag aaagctcttg aagcagtatt
     6841 tggcaaatat ggacgaatag tggaagtact cttgatgaaa gaccgtgaaa ccaacaaatc
     6901 aagaggattt gcttttgtca cctttgaaag cccagcagac gctaaggatg cagccagaga
     6961 catgaatgga aagtcattag atggaaaagc catcaaggtg gaacaagcca ccaaaccatc
     7021 atttgaaagt ggtagacgtg gaccgcctcc acctccaaga agtagaggcc ctccaagagg
     7081 tcttagaggt ggaagaggag gaagtggagg aaccagggga cctccctcac ggggaggaca
     7141 catggatgac ggtggatatt ccatgaattt taacatgagt tcttccaggg gaccactccc
     7201 agtaaaaaga ggaccaccac caagaagtgg gggtcctcct cctaagagat ctgcaccttc
     7261 aggaccagtt cgcagtagca gtggaatggg aggaagagct cctgtatcac gtggaagaga
     7321 tagttatgga ggtccacctc gaagggaacc gctgccctct cgtagagatg tttatttgtc
     7381 cccaagagat gatgggtatt ctactaaaga cagctattca agcagagatt acccaagttc
     7441 tcgtgatact agagattatg caccaccacc acgagattat acttaccgtg attatggtca
     7501 ttccagttca cgtgatgact atccatcaag aggatatagc gatagagatg gatatggtcg
     7561 tgatcgtgac tattcagatc atccaagtgg aggttcctac agagattcat atgagagtta
     7621 tggtaactca cgtagtgctc cacctacacg agggcccccg ccatcttatg gtggaagcag
     7681 tcgctatgat gattacagca gctcacgtga cggatatggt ggaagtcgag acagttactc
     7741 aagcagccga agtgatctct actcaagtgg tcgtgatcgg gttggcagac aagaaagagg
     7801 gcttccccct tctatggaaa gggggtaccc tcctccacgt gattcctaca gcagttcaag
     7861 ccgcggagca ccaagaggtg gtggccgtgg aggaagccga tctgatagag ggggaggcag
     7921 aagcagatac aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt
     7981 ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     8041 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8101 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8161 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8221 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8281 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8401 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8521 gcgggaagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacactcag
     9361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    10501 gatcgatctc ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag
    10561 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    10621 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    10681 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
    10741 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    10801 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    10861 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    10921 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    10981 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    11041 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    11101 accatg
//
