LOCUS       sequence-32076-e         935 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Colbert CL, Wu Q, Erbel PJ, Gardner KH, Deisenhofer J
  TITLE     Mechanism of substrate specificity in Bacillus subtilis ResA, a 
            thioredoxin-like protein involved in cytochrome c maturation.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Mar 21. 103(12):4410-5.
  PUBMED    16537372
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Mar 21. 103(12):4410-5."
COMMENT     Sequence Label: phtsResA
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             492..514
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    529..553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(553..572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(554..572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(656..675)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtcccgaag
       61 tctctccggg ttttatgaga ttcatataat cgtgtatcat gctttctgtc atagtgccgg
      121 tcaccacctt gacaactttt ccttccggat tgatcaaaaa ggttgtcgga agcggagata
      181 cgtcataggc atcaagcact tggcgatctg tatccagaac aaccgggaaa ttgactccgt
      241 aggatttcat aaaattatgt actgctatct ttgactcccc aacatttaca gcgacaattt
      301 caacaccttg gcttttaaaa tgcttatatt ggtttgccat ataaggaaac tcttttttgc
      361 acggttcaca ccatgtaccc cagaaattca aaaaaacacc tttccctttt aagtcactga
      421 gctcgatacg tttcccattc gtatcctcta ggacaaaatt cggtgcgtcg gacccttcgg
      481 acatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc
      541 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc
      601 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
      661 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
      721 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
      781 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
      841 ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa
      901 aacctttcgc ggtatggcat gatagcgccc ggaag
//
