LOCUS       sequence-321549-       15160 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of crRNA array and FnCas12a using one Pol II promoter. 
            For plant genome editing. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Ding D, Chen K, Chen Y, Li H, Xie K
  TITLE     Engineering Introns to Express RNA Guides for Cas9- and 
            Cpf1-Mediated Multiplex Genome Editing.
  JOURNAL   Mol Plant. 2018 Apr 2;11(4):542-552. doi: 
            10.1016/j.molp.2018.02.005. Epub 2018 Feb 17.
  PUBMED    29462720
REFERENCE   2  (bases 1 to 15160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2018.02.005"; journalName: "Mol Plant"; date: 
            "2018-04-2- 2"; volume: "11"; issue: "4"; pages: "542-552"
COMMENT     Sequence Label: p33Fn
FEATURES             Location/Qualifiers
     source          1..15160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    44..65
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(80..85,86..103,104..110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     123..145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     142..158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     142..158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1925..1949
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1994..2059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2066..2086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6008..6055
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(6088..6112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6123..6146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      6168..6420
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6429..6446)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6429..6445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6438..6460)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6648..6672
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7973..8602
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9031..10104
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10170..10364
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10600..10622)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10708..10848
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10763..10782
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10863..10880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11034..11622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11114..11133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11709..12503)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12928..12952
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13030..13204
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13245..14270)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14337..15013)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14380..14400)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
       61 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      121 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgcca agcttacaaa
      181 ttcgggtcaa ggcggaagcc agcgcgccac cccacgtcag caaatacgga ggcgcggggt
      241 tgacggcgtc acccggtcct aacggcgacc aacaaaccag ccagaagaaa ttacagtaaa
      301 aaaaaagtaa attgcacttt gatccacctt ttattaccta agtctcaatt tggatcaccc
      361 ttaaacctat cttttcaatt tgggccgggt tgtggtttgg actaccatga acaacttttc
      421 gtcatgtcta acttcccttt cagcaaacat atgaaccata tatagaggag atcggccgta
      481 tactagagct gatgtgttta aggtcgttga ttgcacgaga aaaaaaaatc caaatcgcaa
      541 caatagcaaa tttatctggt tcaaagtgaa aagatatgtt taaaggtagt ccaaagtaaa
      601 acttatagat aataaaatgt ggtccaaagc gtaattcact caaaaaaaat caacgagacg
      661 tgtaccaaac ggagacaaac ggcatcttct cgaaatttcc caaccgctcg ctcgcccgcc
      721 tcgtcttccc ggaaaccgcg gtggtttcag cgtggcggat tctccaagca gacggagacg
      781 tcacggcacg ggactcctcc caccacccaa ccgccataaa taccagcccc ctcatctcct
      841 ctcctcgcat cagctccacc cccgaaaaat ttctccccaa tctcgcgagg ctctcgtcgt
      901 cgaatcgaat cctctcgcgt cctcaaggta cgctgcttct cctctcctcg cttcgtttcg
      961 attcgatttc ggacgggtga ggttgttttg ttgctagatc cgattggtgg ttagggttgt
     1021 cgatgtgatt atcgtgagat gtttaggggt tgtagatctg atggttgtga tttgggcacg
     1081 gttggttcga taggtggaat cgtggttagg ttttgggatt ggatgttggt tctgatgatt
     1141 ggggggaatt tttacggtta gatgaattgt tggatgattc gattggggaa atcggtgtag
     1201 atctgttggg gaattgtgga actagtcatg cctgagtgat tggtgcgatt tgtagcgtgt
     1261 tccatcttgt aggccttgtt gcgagcatgt tcagatctac tgttccgctc ttgattgagt
     1321 tattggtgcc atgggttggt gcaaacacag gctttaatat gttatatctg ttttgtgttt
     1381 gatgtagatc tgtagggtag ttcttcttag acatggttca attatgtagc ttgtgcgttt
     1441 cgatttgatt tcatatgttc acagattaga taatgatgaa ctcttttaat taattgtcaa
     1501 tggtaaatag gaagtcttgt cgctatatct gtcataatga tctcatgtta ctatctgcca
     1561 gtaatttatg ctaagaacta tattagaata tcatgttaca atctgtagta atatcatgtt
     1621 acaatctgta gttcatctat ataatctatt gtggtaattt ctttttacta tctgtgtgaa
     1681 gattattgcc actagttcat tctacttatt tctgaagttc aggatacgtg tgctgttact
     1741 acctatctga atacatgtgt gatgtgcctg ttactatctt tttgaataca tgtatgttct
     1801 gttggaatat ggcaggagac cgaggtctcg gtttgctgtt tgatccgttg ttgtgtcctt
     1861 aatcttgtgc tagttcttac cctatctgtt tggtgattat ttcttgcaga ttctagagtt
     1921 atcaacaagt ttgtacaaaa aagcaggctt cgaaggagat agaaccaatt ctctaaggaa
     1981 atacttaacc atggattata aggatcatga tggagattac aaggatcacg atattgatta
     2041 taaggatgat gatgataaga tggctcctaa gaagaagaga aaggttggca ttcatggtgt
     2101 gccggctgcc tctatctacc aggagttcgt taataagtac tctttgtcaa agaccctcag
     2161 gtttgagctt attcctcaag gcaagacttt ggaaaacatc aaggccagag gtcttatttt
     2221 ggatgatgag aagagggcta aggattacaa gaaggccaag cagatcattg ataagtacca
     2281 ccaattcttt atcgaggaaa ttctttcttc agtgtgcatc tcagaagatc tccttcagaa
     2341 ttactctgat gtctatttca agttgaagaa gtcagatgat gataacctcc agaaggattt
     2401 taagtctgct aaggatacta tcaagaagca aatttcagag tacatcaagg attctgaaaa
     2461 gtttaagaat ttgttcaacc agaatctcat tgatgccaag aagggccaag agtcagatct
     2521 catcctttgg ttgaagcaat ctaaggataa tggtatcgaa cttttcaagg ctaactcaga
     2581 tatcacagat attgatgagg ccttggaaat cattaagtct tttaagggct ggactacata
     2641 ttttaagggt ttccatgaga acagaaagaa tgtctactct tcaaacgata ttcctacttc
     2701 tatcatctac aggatcgttg atgataattt gccaaagttc ctcgagaaca aggctaagta
     2761 cgaatctctc aaggataagg ctccagaggc cattaattac gaacagatca agaaggatct
     2821 tgccgaggaa ttgacattcg atattgatta caagacctca gaggttaacc agagagtgtt
     2881 ttctttggat gaggtgttcg aaatcgctaa cttcaacaat tatctcaacc aatcaggcat
     2941 taccaagttc aatactatca ttggtggcaa gtttgtcaac ggagagaata caaagaggaa
     3001 gggcattaac gaatacatca atttgtactc tcagcaaatt aacgataaga cactcaagaa
     3061 gtacaagatg tcagtgcttt tcaagcagat cttgtctgat accgagtcta agtcatttgt
     3121 cattgataag ctcgaagatg attcagatgt tgtgaccact atgcagtctt tttacgagca
     3181 aatcgctgcc ttcaagacag ttgaggaaaa gtcaattaag gaaacccttt ctttgctctt
     3241 cgatgatttg aaggctcaaa agctcgatct ttcaaagatc tatttcaaga acgataagtc
     3301 acttaccgat ttgtctcagc aagtgtttga tgattactct gtcattggta ctgctgttct
     3361 tgagtatatt acacagcaaa tcgccccgaa gaaccttgat aatccttcaa agaaggagca
     3421 ggaattgatc gctaagaaga ctgagaaggc caagtacttg tctctcgaaa ctatcaagct
     3481 cgctttggag gagttcaaca agcatagaga tattgataag caatgcaggt ttgaggaaat
     3541 cctcgccaac ttcgctgcca tcccgatgat ttttgatgag atcgctcaga acaaggataa
     3601 tcttgcccaa atctcaatta agtatcagaa ccaaggaaag aaggatcttt tgcaggcttc
     3661 tgccgaagat gatgttaagg ctattaagga tctccttgat cagaccaaca atttgctcca
     3721 taagctcaag atcttccaca tttctcaatc agaggataag gctaacatcc ttgataagga
     3781 tgaacacttc tacttggttt tcgaggaatg ttatttcgag cttgccaaca ttgtgccgtt
     3841 gtacaacaag atcagaaact acatcactca gaagccttac tcagatgaga agtttaagct
     3901 caacttcgaa aattctaccc ttgctaacgg ttgggataag aataaggagc cagataacac
     3961 tgccatcctc ttcattaagg atgataagta ctatcttgga gtgatgaata agaagaacaa
     4021 taagatcttc gatgataagg ctattaagga gaacaagggc gaaggttaca agaagatcgt
     4081 ttacaagctc ttgcctggag ctaataagat gttgccaaag gtgttctttt cagccaagtc
     4141 tattaagttc tacaacccat ctgaggatat cctcagaatt aggaatcatt caacacacac
     4201 caagaacgga tctccgcaga agggctacga gaagtttgag ttcaacatcg aggattgcag
     4261 aaagtttatt gatttctata agcaatctat ctcaaagcat cctgagtgga aggattttgg
     4321 cttcagattt tcagatactc agaggtacaa ctctattgat gagttctata gggaggttga
     4381 aaatcaaggt tacaagctca cattcgagaa catctctgaa tcatacattg attctgtcgt
     4441 taaccaggga aagctctacc ttttccaaat ctacaacaag gatttttcag cttactctaa
     4501 gggcagacca aatctccaca ctctttattg gaaggccctc ttcgatgaga ggaatcttca
     4561 ggatgtggtc tacaagttga acggagaggc tgaactcttt tatagaaagc aatcaattcc
     4621 aaagaagatc acacatccgg ccaaggaggc tatcgccaac aagaataagg ataatcctaa
     4681 gaaggagtct gtgttcgaat acgatcttat taaggataag aggtttacag aggataagtt
     4741 ctttttccac tgtccaatca ccattaactt caagtcttca ggcgctaaca agtttaatga
     4801 tgagatcaat ctccttttga aggaaaaggc caacgatgtt catatcctct ctattgatag
     4861 aggagagagg cacctcgctt actatacact tgtggatgga aagggcaata tcattaagca
     4921 ggatactttc aacatcattg gcaatgatag gatgaagaca aactaccatg ataagctcgc
     4981 tgccattgaa aaggatagag attcagccag gaaggattgg aagaagatca acaatattaa
     5041 ggagatgaag gaaggctacc tctctcaagt tgtgcacgag attgctaagc tcgttatcga
     5101 atataatgcc attgtcgttt tcgaggatct caatttcggt tttaagagag gaaggtttaa
     5161 ggtcgaaaag caggtttacc aaaagctcga gaagatgctt atcgaaaagt tgaactatct
     5221 cgtctttaag gataacgagt tcgataagac cggtggagtt ttgagggctt accagctcac
     5281 agccccgttt gaaaccttca agaagatggg caagcaaact ggaatcatct actatgtccc
     5341 ggctggattc acctctaaga tctgccctgt gactggcttt gtcaatcagc tctaccctaa
     5401 gtatgagtct gtttcaaagt ctcaagaatt tttctcaaag ttcgataaga tctgttacaa
     5461 tcttgataag ggttacttcg agttttcttt cgattacaag aacttcggag ataaggctgc
     5521 caagggaaag tggactattg cttcattcgg atctagactt atcaacttca gaaactctga
     5581 taagaaccat aactgggata ccagagaggt ctatccaact aaggagttgg aaaagctcct
     5641 taaggattac tctatcgagt atggtcacgg agaatgcatt aaggctgcca tctgtggaga
     5701 gtcagataag aagtttttcg ctaagttgac ttctgtgctc aatacaatcc ttcagatgag
     5761 aaactcaaag actggtacag aacttgatta cttgatttct ccggtggctg atgtcaacgg
     5821 aaacttcttc gattctaggc aggcccctaa gaatatgcca caagatgctg atgccaacgg
     5881 cgcctaccac atcggactca agggccttat gttgctcggt aggattaaga acaatcaaga
     5941 gggaaagaag ttgaatctcg tgattaagaa cgaggaatat tttgagttcg tccagaacag
     6001 aaacaataag aggcctgctg ccacaaagaa ggctggtcaa gctaagaaga agaagtgaga
     6061 attcgcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtg gttgataaca
     6121 gcgactacaa ggatgacgat gacaaggctt agagctcgaa tttccccgat cgttcaaaca
     6181 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     6241 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     6301 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     6361 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     6421 gggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6481 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6541 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     6601 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     6661 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     6721 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     6781 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     6841 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     6901 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     6961 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     7021 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     7081 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     7141 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     7201 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     7261 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     7321 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     7381 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     7441 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     7501 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     7561 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     7621 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     7681 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     7741 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     7801 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     7861 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     7921 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     7981 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     8041 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     8101 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     8161 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     8221 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     8281 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     8341 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     8401 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     8461 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     8521 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     8581 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     8641 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     8701 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     8761 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     8821 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     8881 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     8941 ttgaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
     9001 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     9061 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     9121 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     9181 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     9241 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     9301 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     9361 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     9421 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     9481 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     9541 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     9601 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     9661 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     9721 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     9781 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     9841 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     9901 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     9961 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    10021 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    10081 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    10141 ggggaaaaag gtcgaaaagc actctttcct gtgaatagca cgtacattgg gaacccaaag
    10201 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    10261 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    10321 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    10381 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    10441 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    10501 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    10561 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10621 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10681 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    10741 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    10801 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    10861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10921 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11041 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11101 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11221 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11281 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11341 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11401 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11461 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11521 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11581 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11641 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    11701 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
    11761 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
    11821 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
    11881 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
    11941 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
    12001 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
    12061 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
    12121 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
    12181 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
    12241 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
    12301 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
    12361 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
    12421 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
    12481 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
    12541 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    12601 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    12661 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    12721 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    12781 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    12841 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    12901 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    12961 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
    13021 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
    13081 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
    13141 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
    13201 gaaactcgag cttgtcgatc gacagatccc ggtcggcatc tactctattt ctttgccctc
    13261 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
    13321 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
    13381 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
    13441 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg tggcgatcct
    13501 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
    13561 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
    13621 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
    13681 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
    13741 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
    13801 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
    13861 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatagcc
    13921 tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
    13981 ccctgtgaac ggcgggagat gcaataggtc aggctctcgc taaactcccc aatgtcaagc
    14041 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
    14101 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
    14161 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
    14221 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc
    14281 cccccggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
    14341 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
    14401 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
    14461 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    14521 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    14581 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    14641 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    14701 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
    14761 ttcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
    14821 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
    14881 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
    14941 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
    15001 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
    15061 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
    15121 ctccccgcgc gttggccgat tcattaatgc agctggcacg
//
