LOCUS       sequence-321899-       12922 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses human CFTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Choi SH, Reeves RE, Romano Ibarra GS, Lynch TJ, Shahin WS, Feng Z, 
            Gasser GN, Winter MC, Evans TIA, Liu X, Luo M, Zhang Y, Stoltz DA, 
            Devor EJ, Yan Z, Engelhardt JF
  TITLE     Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal 
            Cells Using miR-106b.
  JOURNAL   Genes (Basel). 2020 Oct 6;11(10). pii: genes11101169. doi: 
            10.3390/genes11101169.
  PUBMED    33036232
REFERENCE   2  (bases 1 to 12922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2020-10-6"; volume: "11(10). pii: genes11101169. 
            doi"; pages: " 10.3390/genes11101169"
COMMENT     Sequence Label: pLV-CBA-dt/PGK-CFTR-⌀
FEATURES             Location/Qualifiers
     source          1..12922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1824..1941
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2023..2247
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2236..2253
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(6910..6929)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7303..7326
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        7387..7766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7768..8045
                     /label=chicken beta-actin promoter
     CDS             8070..8771
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(8203..8221)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8471..8490
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8665..8688
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    8782..9370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8835..8855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9253..9264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9443..9676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9748..9869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9785..9804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9839..9858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9909..10044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10064..10083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10065..10083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10093..10110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10093..10109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10102..10124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10251..10706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10338..10357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10548..10569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10732..10836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10837..10905,10906..11697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11055..11074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11868..12456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12357..12376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12610..12627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12744..12765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12780..12785,12786..12803,12804..12810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12818..12834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12823..12845
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12842..12858
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12842..12858
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12877..12897
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12879..12897
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 caaatggcag tattcattca caattttaaa agaaaagggg ggattggggg gtacagtgca
     1861 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     1921 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggtct
     1981 agtggatcct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
     2041 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     2101 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     2161 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     2221 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcttatcgac aacggcatcg
     2281 ctactctagc ctgccaattc catgagcaaa tgtcccatgt caacatttat gctgctctct
     2341 aaagccttgt atcttgcacc tcttcttctg tctcctcttt cagagcagca atctggggct
     2401 tagacttgca cttgcttgag ttccggtggg gaaagagctt caccctgtcg gaggggctga
     2461 tggcttgccg gaagaggctc ctctcgttca gcagtttctg gatggaatcg tactgccgca
     2521 ctttgttctc ttctatgacc aaaaattgtt ggcattccag cattgcttct atcctgtgtt
     2581 cacagagaat tactgtgcaa tcagcaaatg cttgttttag agttcttcta attatttggt
     2641 atgttactgg atccaaatga gcactgggtt catcaagcag caagatcttc gccttactga
     2701 gaacagatct agccaagcac atcaactgct tgtggccatg gcttaggaca cagcccccat
     2761 ccacaaggac aaagtcaagc ttcccaggaa actgttctat cacagatctg agcccaacct
     2821 catctgcaac tttccatatt tcttgatcac tccactgttc atagggatcc aagttttttc
     2881 taaatgttcc agaaaaaata aatactttct gtggtatcac tccaaaggct ttcctccact
     2941 gttgcaaagt tattgaatcc caagacacac catcgatctg gatttctcct tcagtgttca
     3001 gtagtctcaa aaaagctgat aacaaagtac tcttccctga tccagttctt cccaagaggc
     3061 ccaccctctg gccaggactt attgagaagg aaatgttctc taatatggca tttccacctt
     3121 ctgtgtattt tgctgtgaga tctttgacag tcatttggcc ccctgagggc cagatgtcat
     3181 ctttcttcac gtgtgaattc tcaataatca taactttcga gagttggcca ttcttgtatg
     3241 gtttggttga cttggtaggt ttaccttctg ttggcatgtc aatgaactta aagactcggc
     3301 tcacagatcg catcaagcta tccacatcta tgctggagtt tacagcccac tgcaatgtac
     3361 tcatgatatt catggctaaa gtcaggataa taccaactct tccttctcct tctcctgttg
     3421 ttaaaatgga aatgaaggta acagcaatga agaagatgac aaaaatcatt tctattctca
     3481 tttggaacca gcgcagtgtt gacaggtaca agaaccagtt ggcagtatgt aaattcagag
     3541 ctttgtggaa cagagtttca aagtaaggct gccgtccgaa ggcacgaagt gtccatagtc
     3601 cttttaagct tgtaacaaga tgagtgaaaa ttggactcct gccttcagat tccagttgtt
     3661 tgagttgctg tgaggtttgg aggaaatatg ctctcaacat aataaaagcc actatcactg
     3721 gcactgttgc aacaaagatg tagggttgta aaactgcgac aactgctata gctccaatca
     3781 caattaataa caactggatg aagtcaaata tggtaagagg cagaaggtca tccaaaattg
     3841 ctatatcttt ggagaatcta ttaagaatcc cacctgcttt caacgtgttg agggttgaca
     3901 taggtgcttg aagaacagaa tgtaacattt tgtggtgtaa aattttcgac actgtgatta
     3961 gagtatgcac cagtggtaga cctctgaaga atcccatagc aagcaaagtg tcggctactc
     4021 ccacgtaaat gtaaaacaca taatacgaac tggtgctggt gataatcact gcatagctgt
     4081 tatttctact atgagtacta ttccctttgt cttgaagagg agtgtttcca aggagccaca
     4141 gcacaaccaa agaagcagcc acctctgcca gaaaaattac taagcaccaa attagcacaa
     4201 aaattaagct cttgtggaca gtaatatatc gaaggtatgt gttccatgta gtcactgctg
     4261 gtatgctctc catatcatca aaaaggcact cctttaagtc ttcttcgtta atttcttcac
     4321 ttatttccaa gccagtttct tgagataacc ttcttgaata tatatccagt tcagtcaagt
     4381 ttgcctgagg ggccagtgac acttttcgtg tggatgctgt tgtctttcgg tgaatgttct
     4441 gaccttggtt aactgagtgt gtcatcaggt tcaggacaga ctgcctcctt cgtgcctgaa
     4501 gcgtggggcc agtgctgatc acgctgatgc gaggcagtat cgcctctccc tgctcagaat
     4561 ctggtactaa ggacagcctt ctctctaaag gctcatcaga atcctcttcg atgccattca
     4621 tttgtaaggg agtcttttgc acaatggaaa attttcgtat agagttgatt ggattgagaa
     4681 tagaattctt ccttttttcc ccaaactctc cagtctgttt aaaagattgt ttttttgttt
     4741 ctgtccagga gacaggagca tctccttcta atgagaaacg gtgtaaggtc tcagttagga
     4801 ttgaatttct tctttctgca ctaaattggt cgaaagaatc acatcccatg agttttgagc
     4861 taaagtctgg ctgtagattt tggagttctg aaaatgtccc ataaaaatag ctgctacctt
     4921 catgcaaaat taatattttg tcagctttct ttaaatgttc cattttagaa gtgaccaaaa
     4981 tcctagtttt gttagccatc agtttacaga cacagctttc aaatatttct ttttctgtta
     5041 aaacatctag gtatccaaaa ggagagtcta ataaatacaa atcagcatct ttgtatactg
     5101 ctcttgctaa agaaattctt gctcgttgac ctccactcag tgtgattcca ccttctccaa
     5161 gaactatatt gtctttctct gcaaacttgg agatgtcctc ttctagttgg catgctttga
     5221 tgacgcttct gtatctatat tcatcatagg aaacaccaaa gatgatattt tctttaatgg
     5281 tgccaggcat aatccaggaa aactgagaac agaatgaaat tcttccactg tgcttaattt
     5341 taccctctga aggctccagt tctcccataa tcatcattag aagtgaagtc ttgcctgctc
     5401 cagtggatcc agcaaccgcc aacaactgtc ctctttctat cttgaaatta atatctttca
     5461 ggacaggagt accaagaagt gagaaattac tgaagaagag gctgtcatca ccattagaag
     5521 tttttctatt gttattgttt tgttttgctt tctcaaataa ttccccaaat ccctcctccc
     5581 agaaggctgt tacattctcc atcactactt ctgtagtcgt taagttatat tccaatgtct
     5641 tatattcttg cttttgtaag aaatcctgta ttttgtttat tgctccaaga gagtcatacc
     5701 atgtttgtac agcccaggga aattgccgag tgaccgccat gcgcagaaca atgcagaatg
     5761 agatggtggt gaatattttc cggaggatga ttcctttgat tagtgcatag ggaagcacag
     5821 ataaaaacac cacaaagaac cctgagaaga agaaggctga gctattgaag tatctcacat
     5881 aggctgcctt ccgagtcagt ttcagttctg tttgtcttaa gttttcaatc attttttcca
     5941 ttgcttcttc ccagcagtat gccttaacag attggatgtt ctcgatcatt tctgaggtaa
     6001 tcacaagtct ttcactgatc ttcccagctc tctgatctct gtacttcatc atcattctcc
     6061 ctagcccagc ctgaaaaagg gcaaggacta tcaggaaacc aagtccacag aaggcagacg
     6121 cctgtaacaa ctcccagatt agccccatga ggagtgccac ttgcaaagga gcgatccaca
     6181 cgaaatgtgc caatgcaagt ccttcatcaa atttgttcag gttgttggaa aggagactaa
     6241 caagttgtcc aatacttatt ttatctagaa cacggcttga cagctttaaa gtcttcttat
     6301 aaatcaaact aaacatagct attctcatct gcattccaat gtgatgaagg ccaaaaatgg
     6361 ctgggtgtag gagcagtgtc ctcacaataa agagaaggca taagcctatg cctagataaa
     6421 tcgcgataga gcgttcctcc ttgttatccg ggtcatagga agctatgatt cttcccagta
     6481 agagaggctg tactgctttg gtgacttccc ctaaatataa aaagattcca tagaacataa
     6541 atctccagaa aaaacatcgc cgaagggcat taatgagttt aggatttttc tttgaagcca
     6601 gctctctatc ccattctctt tccaattttt cagatagatt gtcagcagaa tcaacagaag
     6661 ggatttggta tatgtctgac aattccaggc gctgtctgta tcctttcctc aaaattggtc
     6721 tggtccagct gaaaaaaagt ttggagacaa cgctggcctt ttccagaggc gacctctgca
     6781 tggtggcggc gccgtctggg ctcaagctcc taatgcggag cacgcgtcag ctgactagag
     6841 gatccccggg taccgacagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc
     6901 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
     6961 cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc
     7021 ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca
     7081 ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca
     7141 cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga
     7201 aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga
     7261 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
     7321 gcatgctcca gactgccttg ggaaaagtac tggacggaat gcatggctgc caatataatt
     7381 catgttggca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     7441 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     7501 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     7561 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     7621 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
     7681 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
     7741 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     7801 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     7861 cagcgatggg ggcggggggg gggggggggc gcgcgccagg sggggcgggg cggggcgagg
     7921 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     7981 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     8041 gggcgggagt caagcttgcc gccggcacca tggtgagcaa gggcgaggag gtcatcaaag
     8101 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     8161 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     8221 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     8281 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     8341 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     8401 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     8461 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     8521 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     8581 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     8641 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     8701 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg
     8761 agctgtacaa gtaactcgag taatcaacct ctggattaca aaatttgtga aagattgact
     8821 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8881 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8941 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9001 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9061 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9121 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     9181 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9241 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9301 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9361 gcctccccgc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9421 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     9481 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9661 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     9721 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     9781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9841 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     9901 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9961 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10021 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
    10081 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    10141 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    10201 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
    10261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    10321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    10381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    10441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    10501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    10561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    10621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
    10681 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
    10741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10801 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    10861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10921 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    11041 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    11101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    11161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    11221 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    11281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    11341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    11401 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    11461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    11521 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    11581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    11641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    11701 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    11821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    11881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    12001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    12061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    12121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    12181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    12241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    12301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    12361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    12421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    12481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    12541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    12601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    12661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    12721 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    12781 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    12841 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    12901 caaaagctgg agctgcaagc tt
//
