LOCUS       sequence-322084-        3945 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Streptomyces phage PhiC31 integrase, complete CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 PhiC31 integrase (GB1496)
FEATURES             Location/Qualifiers
     source          1..3945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..1816
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             1817..1837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1882..1939
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1963..1980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2134..2722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2214..2233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2905..2999)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3020..3679)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3594..3613
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3841..3859
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3863..3906)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3909..3930
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggacacg tatgccggcg cttatgatag acaatcaaga gaaagagaga actcttctgc
       61 cgcctctcca gctacacaga gatctgccaa cgaggataaa gctgctgatt tacaaagaga
      121 ggtagagaga gatgggggta ggtttagatt tgtgggtcat ttctctgagg ctcctggtac
      181 atcagccttt ggtactgctg aacggcctga atttgaaaga atattaaatg agtgtcgggc
      241 cggtcgacta aatatgataa tagtttatga tgtatctagg ttcagtagac tcaaagttat
      301 ggatgcaatt ccgatcgtga gcgagctact tgcactcggt gttactatcg taagtacaca
      361 agaaggtgta ttcaggcagg gtaatgtgat ggaccttata caccttatta tgcgactcga
      421 cgctagccat aaagagtcat cactgaagag tgctaaaatc ttggacacca agaacctgca
      481 aagggagctt ggaggctatg taggaggaaa agctccgtat ggtttcgaac tcgtgagtga
      541 gacaaaagag attaccagaa atggtaggat ggtgaacgtg gttattaata agctagctca
      601 tagcacaacc cctctcacag ggccttttga gttcgaacct gacgttatta gatggtggtg
      661 gagagagatc aaaactcaca aacatcttcc atttaagcca ggtagccagg ctgctatcca
      721 ccctggatct atcactgggc tttgtaaaag gatggatgct gacgcagtgc caactcgagg
      781 ggaaacaatc ggtaagaaga ctgcatcttc cgcttgggat cccgctacag ttatgagaat
      841 ccttcgggat ccgcgtattg ctggattcgc cgcagaagtc atttataaga aaaaacctga
      901 tggtactcct actacaaaaa tcgaaggtta taggatccaa agggatccta ttactttgcg
      961 tccagttgaa ttagactgtg gaccgatcat cgaacctgcc gagtggtacg agttgcaggc
     1021 ctggcttgat ggtagagggc gtggaaaggg tttatctcgg ggacaggcaa ttttgtcagc
     1081 aatggataag ctttattgtg agtgtggggc tgttatgaca tcaaaacgtg gagaagagtc
     1141 aatcaaggat tcttacaggt gcagacgtcg taaagtggtg gacccttctg ctccaggaca
     1201 gcatgagggt acatgcaacg tttctatggc tgctcttgat aaatttgtgg ccgaacgtat
     1261 tttcaacaag attagacacg ctgaagggga tgaggaaact ttagctcttc tttgggaggc
     1321 tgcccggcgt tttggtaagc taacagaggc accagaaaaa agtggcgaaa gagctaattt
     1381 ggtagccgaa agagcagacg ccttgaatgc attggaagaa ctttacgaag atagggctgc
     1441 cggagcatat gatggaccag ttggtcggaa gcatttcagg aagcaacaag ctgcactaac
     1501 tttgagacag caaggcgcag aggagcgact cgcagagctt gaggccgccg aggccccaaa
     1561 gcttccactc gatcaatggt ttcctgagga tgccgacgct gatcctacag gtccaaagag
     1621 ctggtgggga agagctagcg tagacgataa gagagtgttt gtgggtctat ttgttgacaa
     1681 gattgtagtt actaagtcca ctacgggtcg aggacagggc actcccatcg aaaagagagc
     1741 cagtattacc tgggcaaagc ctccaaccga tgatgatgaa gacgatgcac aagatggtac
     1801 cgaggatgtg gcagcaccaa aaaaaaagag gaaggtgtaa gctttgagac cactcatcgc
     1861 gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     1921 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     1981 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2101 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     2161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2761 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2821 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2881 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     2941 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     3001 aagcgagctc gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt
     3061 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     3121 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     3181 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     3241 ctgatacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     3301 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     3361 tccagagtga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     3421 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     3481 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     3541 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     3601 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     3661 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     3721 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     3781 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3841 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     3901 gatttctgga attcgcggcc gcttctagag cgatgagtgg tctcg
//
