LOCUS       sequence-322223-       12422 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentivirus delivery for stable expression of AsCas12a-VPR, Zeocin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12422)
  TITLE     Karreth lab plasmids
REFERENCE   2  (bases 1 to 12422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMB22.SV40.Zeo
FEATURES             Location/Qualifiers
     source          1..12422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2678..2889
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2932..2941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2944..2964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2989..6906
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      6626..6635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6907..6954
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6988..7137
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7162..7182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7612..7968
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7987..8556
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     primer_bind     complement(8576..8596)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8581..8910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8761..8896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8920..9294
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9310..9898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9363..9383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9781..9792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9901..9917)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9902..9918)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10427..10607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10669..11257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10749..10768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11428..12219,12220..12288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12051..12070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12289..12393)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gggtcgacta gtgatcagtg tgagggagtg
     2641 taaagctggt tttcgagtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2701 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2761 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2821 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2881 agaacacagg tgtcgtgacg cggctaactc gaacgctagc tgtgcgatcg tgccaccatg
     2941 gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccac acagttcgag
     3001 ggctttacca acctgtatca ggtgagcaag acactgcggt ttgagctgat cccacagggc
     3061 aagaccctga agcacatcca ggagcagggc ttcatcgagg aggacaaggc ccgcaatgat
     3121 cactacaagg agctgaagcc catcatcgat cggatctaca agacctatgc cgaccagtgc
     3181 ctgcagctgg tgcagctgga ttgggagaac ctgagcgccg ccatcgactc ctatagaaag
     3241 gagaaaaccg aggagacaag gaacgccctg atcgaggagc aggccacata tcgcaatgcc
     3301 atccacgact acttcatcgg ccggacagac aacctgaccg atgccatcaa taagagacac
     3361 gccgagatct acaagggcct gttcaaggcc gagctgttta atggcaaggt gctgaagcag
     3421 ctgggcaccg tgaccacaac cgagcacgag aacgccctgc tgcggagctt cgacaagttt
     3481 acaacctact tctccggctt ttatgagaac aggaagaacg tgttcagcgc cgaggatatc
     3541 agcacagcca tcccacaccg catcgtgcag gacaacttcc ccaagtttaa ggagaattgt
     3601 cacatcttca cacgcctgat caccgccgtg cccagcctgc gggagcactt tgagaacgtg
     3661 aagaaggcca tcggcatctt cgtgagcacc tccatcgagg aggtgttttc cttccctttt
     3721 tataaccagc tgctgacaca gacccagatc gacctgtata accagctgct gggaggaatc
     3781 tctcgggagg caggcaccga gaagatcaag ggcctgaacg aggtgctgaa tctggccatc
     3841 cagaagaatg atgagacagc ccacatcatc gcctccctgc cacacagatt catccccctg
     3901 tttaagcaga tcctgtccga taggaacacc ctgtctttca tcctggagga gtttaagagc
     3961 gacgaggaag tgatccagtc cttctgcaag tacaagacac tgctgagaaa cgagaacgtg
     4021 ctggagacag ccgaggccct gtttaacgag ctgaacagca tcgacctgac acacatcttc
     4081 atcagccaca agaagctgga gacaatcagc agcgccctgt gcgaccactg ggatacactg
     4141 aggaatgccc tgtatgagcg gagaatctcc gagctgacag gcaagatcac caagtctgcc
     4201 aaggagaagg tgcagcgcag cctgaagcac gaggatatca acctgcagga gatcatctct
     4261 gccgcaggca aggagctgag cgaggccttc aagcagaaaa ccagcgagat cctgtcccac
     4321 gcacacgccg ccctggatca gccactgcct acaaccctga agaagcagga ggagaaggag
     4381 atcctgaagt ctcagctgga cagcctgctg ggcctgtacc acctgctgga ctggtttgcc
     4441 gtggatgagt ccaacgaggt ggaccccgag ttctctgccc ggctgaccgg catcaagctg
     4501 gagatggagc cttctctgag cttctacaac aaggccagaa attatgccac caagaagccc
     4561 tactccgtgg agaagttcaa gctgaacttt cagatgccta cactggcctc tggctgggac
     4621 gtgaataagg agaagaacaa tggcgccatc ctgtttgtga agaacggcct gtactatctg
     4681 ggcatcatgc caaagcagaa gggcaggtat aaggccctga gcttcgagcc cacagagaaa
     4741 accagcgagg gctttgataa gatgtactat gactacttcc ctgatgccgc caagatgatc
     4801 ccaaagtgca gcacccagct gaaggccgtg acagcccact ttcagaccca cacaaccccc
     4861 atcctgctgt ccaacaattt catcgagcct ctggagatca caaaggagat ctacgacctg
     4921 aacaatcctg agaaggagcc aaagaagttt cagacagcct acgccaagaa aaccggcgac
     4981 cagaagggct acagagaggc cctgtgcaag tggatcgact tcacaaggga ttttctgtcc
     5041 aagtatacca agacaacctc tatcgatctg tctagcctgc ggccatcctc tcagtataag
     5101 gacctgggcg agtactatgc cgagctgaat cccctgctgt accacatcag cttccagaga
     5161 atcgccgaga aggagatcat ggatgccgtg gagacaggca agctgtacct gttccagatc
     5221 tataacaagg actttgccaa gggccaccac ggcaagccta atctgcacac actgtattgg
     5281 accggcctgt tttctccaga gaacctggcc aagacaagca tcaagctgaa tggccaggcc
     5341 gagctgttct accgccctaa gtccaggatg aagaggatgg cacaccggct gggagagaag
     5401 atgctgaaca agaagctgaa ggatcagaaa accccaatcc ccgacaccct gtaccaggag
     5461 ctgtacgact atgtgaatca cagactgtcc cacgacctgt ctgatgaggc cagggccctg
     5521 ctgcccaacg tgatcaccaa ggaggtgtct cacgagatca tcaaggatag gcgctttacc
     5581 agcgacaagt tctttttcca cgtgcctatc acactgaact atcaggccgc caattcccca
     5641 tctaagttca accagagggt gaatgcctac ctgaaggagc accccgagac acctatcatc
     5701 ggcatcgatc ggggcgagag aaacctgatc tatatcacag tgatcgactc caccggcaag
     5761 atcctggagc agcggagcct gaacaccatc cagcagtttg attaccagaa gaagctggac
     5821 aacagggaga aggagagggt ggcagcaagg caggcctggt ctgtggtggg cacaatcaag
     5881 gatctgaagc agggctatct gagccaggtc atccacgaga tcgtggacct gatgatccac
     5941 taccaggccg tggtggtgct ggagaacctg aatttcggct ttaagagcaa gaggaccggc
     6001 atcgccgaga aggccgtgta ccagcagttc gagaagatgc tgatcgataa gctgaattgc
     6061 ctggtgctga aggactatcc agcagagaaa gtgggaggcg tgctgaaccc ataccagctg
     6121 acagaccagt tcacctcctt tgccaagatg ggcacccagt ctggcttcct gttttacgtg
     6181 cctgccccat atacatctaa gatcgatccc ctgaccggct tcgtggaccc cttcgtgtgg
     6241 aaaaccatca agaatcacga gagccgcaag cacttcctgg agggcttcga ctttctgcac
     6301 tacgacgtga aaaccggcga cttcatcctg cactttaaga tgaacagaaa tctgtccttc
     6361 cagaggggcc tgcccggctt tatgcctgca tgggatatcg tgttcgagaa gaacgagaca
     6421 cagtttgacg ccaagggcac ccctttcatc gccggcaaga gaatcgtgcc agtgatcgag
     6481 aatcacagat tcaccggcag ataccgggac ctgtatcctg ccaacgagct gatcgccctg
     6541 ctggaggaga agggcatcgt gttcagggat ggctccaaca tcctgccaaa gctgctggag
     6601 aatgacgatt ctcacgccat cgacaccatg gtggccctga tccgcagcgt gctgcagatg
     6661 cggaactcca atgccgccac aggcgaggac tatatcaaca gccccgtgcg cgatctgaat
     6721 ggcgtgtgct tcgactcccg gtttcagaac ccagagtggc ccatggacgc cgatgccaat
     6781 ggcgcctacc acatcgccct gaagggccag ctgctgctga atcacctgaa ggagagcaag
     6841 gatctgaagc tgcagaacgg catctccaat caggactggc tggcctacat ccaggagctg
     6901 cgcaacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc
     6961 aaagaggcca gcggttccgg acgggctgac gcattggacg attttgatct ggatatgctg
     7021 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
     7081 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
     7141 aactctagaa gttccggatc tccgaaaaag aaacgcaaag ttggtagcca gtacctgccc
     7201 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
     7261 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
     7321 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
     7381 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
     7441 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
     7501 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
     7561 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
     7621 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
     7681 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
     7741 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
     7801 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
     7861 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
     7921 gacgaggact tcagctctat cgccgatatg gatttctcag ccttgctggg ctctggcagc
     7981 ggcagccggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     8041 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     8101 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     8161 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     8221 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agagacgagc
     8281 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     8341 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     8401 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     8461 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     8521 agcacaggac tgtccatctt cgacacatct ctgttttgac ctaggtcgac cctgtggaat
     8581 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8641 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8701 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8881 ggcttttttg gaggcctagg cttttgcaaa aagcttacca tggccaagtt gaccagtgcc
     8941 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     9001 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     9061 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     9121 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     9181 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     9241 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgaatcggc
     9301 gcgcccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     9361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     9421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     9721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9841 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     9901 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     9961 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
    10021 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
    10081 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
    10141 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
    10201 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
    10261 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
    10321 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
    10381 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
    10441 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10501 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10561 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
    10621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    12421 cg
//
