LOCUS       sequence-322398-        5592 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Luciferase reporter for mouse Cxcl1 with Chantico promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pCxcl1-Firefly-Chantico
FEATURES             Location/Qualifiers
     source          1..5592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             283..1935
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1979..2100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1990..2009)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2044..2063
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3471..3488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3642..4230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3722..3741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4430..5221,5222..5290))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5395..5443
                     /note="synthetic polyadenylation signal"
     misc_feature    5457..5548
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5497..5516
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ctgaagtctc actactcctc ccccccccaa ccccccagcc gtcacttgtc cagcgaagca
       61 cgtcactccc tcccctttgg gactttgggg caaaaagcaa aaatcccaga gtctaatcct
      121 tgggagtgga gcaaggggga ggagcgatgt cctttccggt tgcagggaaa caccctgtac
      181 tccgggaatt tccctggccc ggagctctgg agtttcgagc ataaaagggg ctcgcgggag
      241 ccctagagct cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac
      301 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
      361 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
      421 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
      481 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
      541 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
      601 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
      661 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
      721 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
      781 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
      841 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
      901 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
      961 ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc
     1021 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     1081 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     1141 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     1201 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     1261 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     1321 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     1381 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     1441 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     1501 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
     1561 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     1621 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     1681 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     1741 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     1801 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     1861 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     1921 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     1981 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2041 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2101 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2161 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccggt cattcagtaa
     2221 atataaatgc agatagccta cgttctgaaa gtggggttct agggtccctt ggttagtttt
     2281 aaaaattcaa agtcactttg agttttgttt tgttttgttt tgttttaaag cttgagagtg
     2341 gccgtgcaga gcaaagttgt ccaattaagt ttatatcagt gacagatggg cttatcagac
     2401 cttcgctctc agacagtagc catggaacac ttagctgctg cctcccgaaa gtgcaagcag
     2461 accagttcag gatctgaagc tttgttttgg tttgacttaa atgtaagtgc aaacagtcgc
     2521 tgtggggtgt gtgtgtgtgt gtgtgtgtga tactggccga tgcaggctga gagtaggggt
     2581 gtctcactcc tgactgcgta ctccccctct gttttacaca ggcttattta ctgttacacg
     2641 gagacttttc ttcgaactgt taaactcttg gctgagtttg tgtggagtat gaggcaaatc
     2701 tctaaccgca agcatgcagt ggaaagatta ggcatacttt tgaaacgagt ttgtgtacca
     2761 gatcatattc ttggctcaag aaagaaaaag aaagggggct ggggaaaggc ttgtggttgc
     2821 tactctggga actaaagaat tcaaaaggct tgttctccat gtttcgtaga cacagacatc
     2881 tggaattgtt gttttaaaat tacaagcaat tgtgtatgct ttgttactgc aacacttatt
     2941 cagaggcaag gacataggtg ccactgttgt cagaaagcca gatgtccttt ccttaaattg
     3001 gcctgcctgg ctgctggcgc ctgggtgtca gcagacactt tgggaatctc agttagcggt
     3061 actgcatgga tatagtgcat ctgcttctca cacttctgcc aaccttgtgt attggtttgc
     3121 ttctctccat ggtgttaaaa caatgactga aagcgtcttg ggtagggcaa gggtatattt
     3181 agcttatagt tgcacatgac agtccatcgt caagggagag catggcggga actcaaggac
     3241 tttgaggagg aactgaagca caggcataga ggctgttcct cactggcttg ctccccaggg
     3301 cttgctcagc tagctgtctt acagagccca gaaccccgat gcccttgaga gccttcaacc
     3361 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     3421 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact
     3481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4321 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4381 gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt taccagtgct
     4441 tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata gtggcctgac
     4501 tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc agcgcagcaa
     4561 tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac cagccagcag
     4621 ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag tctatgagct
     4681 gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga gttgtggcca
     4741 ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc aactctggtt
     4801 cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca gtcagctcct
     4861 tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc atggtaatgg
     4921 cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc gtgaccggcg
     4981 agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc tcttgcccgg
     5041 cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc atcatcggga
     5101 atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc agttcgatat
     5161 agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc gtttcggggt
     5221 gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt
     5281 ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg
     5341 tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa
     5401 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     5461 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     5521 gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtacctga gctcgctagc
     5581 ctcgaggata tc
//
