LOCUS       sequence-322399-       11232 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Booster-PKA for the generation of mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Watabe T, Terai K, Sumiyama K, Matsuda M
  TITLE     Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy 
            Transfer (FRET) Biosensor Compatible with Cyan Fluorescent 
            Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue 
            Light-Responsive Optogenetic Tools.
  JOURNAL   ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.
  PUBMED    32101394
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2020 Feb 26. doi: 10.1021/acssensors.9b01941."
COMMENT     Sequence Label: pT2ADW-lox-mCherryNLS-pA-lox-4493NES
FEATURES             Location/Qualifiers
     source          1..11232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        3819..4198
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4200..4477
                     /label=chicken beta-actin promoter
     intron          4478..5494
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5418..5440
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5502..5521
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(5548..5581)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5738..5756)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6006..6025
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6301..6321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6334..6353)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6399..6454
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6453..6472)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(6803..6836)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6848..6857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6854..7549
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             8723..9376
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             9422..9448
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    9472..10060
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9525..9545)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9943..9954)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10125..10259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10149..10168)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10203..10222
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(10585..10604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10586..10604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10614..10631)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10614..10630)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10623..10645)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10772..11199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10858..10877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11068..11089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg atcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcggatctg ctgggcttgc
     2221 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     2281 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
     2341 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
     2401 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggccctct agatgcatgc
     2461 cggctggccc aggccatcgg cattccggag cccagggtcc agatttggtt tcagaatgag
     2521 aggtcacgcc agctgaggca gcaccggcgg gaatctcggc cctggcccgg gagacgcggc
     2581 ccgccagaag gccggcgaaa gcggaccgcc gtcaccggat cccagaccgc cctgctcctc
     2641 cgagcctttg agaaggatcg ctttccaggc atcgccgccc gggaggagct ggccagagag
     2701 acgggcctcc cggagtccag gattcagatc tggtttcaga atcgaagggc caggcacccg
     2761 ggacagggtg gcagggcgcc cgcgcaggca ggcggcctgt gcagcgcggc ccccggcggg
     2821 ggtcaccctg ctccctcgtg ggtcgccttc gcccacaccg gcgcgtgggg aacggggctt
     2881 cccgcacccc acgtgccctg cgcgcctggg gctctcccac agggggcttt cgtgagccag
     2941 gcagcgaggg ccgcccccgc gctgcagccc agccaggccg cgccggcaga ggggatctcc
     3001 caacctgccc cggcgcgcgg ggatttcgcc tacgccgccc cggctcctcc ggacggggcg
     3061 ctctcccacc ctcaggctcc tcgctggcct ccgcacccgg gcaaaagccg ggaggaccgg
     3121 gacccgcagc gcgacggcct gccgggcccc tgcgcggtgg cacagcctgg gcccgctcaa
     3181 gcggggccgc agggccaagg ggtgcttgcg ccacccacgt cccaggggag tccgtggtgg
     3241 ggctggggcc ggggtcccca ggtcgccggg gcggcgtggg aaccccaagc cggggcagct
     3301 ccacctcccc agcccgcgcc cccggacgcc tccgcctccg cgcggcaggg gcagatgcaa
     3361 ggcatcccgg cgccctccca ggcgctccag gagccggcgc cctggtctgc actcccctgc
     3421 ggcctgctgc tggatgagct cctggcgagc ccggagtttc tgcagcaggc gcaacctctc
     3481 ctagaaacgg aggccccggg ggagctggag gcctcggaag aggccgcctc gctggaagca
     3541 cccctcagcg aggaagaata ccgggctctg ctggaggagc tttaggacgc ggggttggga
     3601 cggggtcggg tggttcgggg cagggccgtg gcctctcttt cgcggggaac acctggctgg
     3661 ctacggaggg gcgtgtctcc gccccgcccc ctccaccggg ctgaccggcc tgggattcct
     3721 gccttctagg tctaggcccg gtgagagact ccacaccgcg gagaactgcc attctttcct
     3781 gggcatcccg gggatcccag agccggccca gcatgctcga cattgattat tgactagtta
     3841 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3901 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3961 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4021 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4081 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4141 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4201 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4261 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     4321 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
     4381 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     4441 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
     4501 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     4561 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     4621 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     4681 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     4741 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     4801 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     4861 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     4921 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     4981 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     5041 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     5101 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     5161 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     5221 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     5281 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     5341 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     5401 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     5461 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     5521 gctgtctcat cattttggca aagaattata acttcgtata gcatacatta tacgaagtta
     5581 ttggctagca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5641 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5701 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5761 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5821 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5881 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5941 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     6001 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     6061 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     6121 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     6181 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6241 gaacagtacg aacgcgccga gggccgccac tccactctcg gcatggacga gctgtctaga
     6301 cctaagaaga agcgtaaggt gtaagtcgac ggatcttttt ccctctgcca aaaattatgg
     6361 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     6421 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     6481 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     6541 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     6601 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     6661 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     6721 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     6781 cctcgacctg cagcccaagc ttataacttc gtatagcata cattatacga agttattgga
     6841 attcgccgcc accatggtga gcgagctgat taaggagaac atgcacatga agctgtacat
     6901 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
     6961 cgagggcacc cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt
     7021 cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg
     7081 catccccgac ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac
     7141 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     7201 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
     7261 gaaaacactc ggctgggagg cctccaccga gaccctgtac cccgctgacg gcggcctgga
     7321 aggcagagcc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa
     7381 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt
     7441 ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
     7501 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacagac tcgagaagtt
     7561 ttctcaagaa cagatcggcg aaaacattgt gtgcagggtc atttgtacca cgggtcaaat
     7621 tcccatccga gatttgtcag ctgatatttc acaagtgctt aaggaaaaac gatccataaa
     7681 gaaagtttgg acatttggta gaaacccagc ctgtgactat catttaggaa acatttcaag
     7741 actgtcaaat aagcatttcc aaatactact aggagaagac ggtaaccttt tattgaatga
     7801 catttccact aatgggacct ggttaaatgg gcaaaaagtc gagaagaaca gcaatcagtt
     7861 actgtcccaa ggtgatgaaa taaccgttgg tgtaggcgtg gaatcagata ttttatctct
     7921 ggtcattttc ataaacgaca aatttaagca gtgcctggag cagaacaaag ttgatcgcgg
     7981 taccagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     8041 tggttccggc agtgctggtg gtagtgctgg tggtagtacc agtgctggtg gtagtgctgg
     8101 tggtagtgct ggtggtagtg ctggtggtag tgctggtggt tccggcagtg ctggtggtag
     8161 tgctggtggt agtaccagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     8221 tggtagtgct ggtggttgcg gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg
     8281 tggtagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gttgtggcag
     8341 tgctggtggt agtgctggtg gtagtaccag tgctggtggt agtgctggtg gtagtgctgg
     8401 tggtagtgct ggtggtagtg ctggtggttg tggcagtgct ggtggtagtg ctggtggtag
     8461 taccagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     8521 tggttccggc agtgctggtg gtagtgctgg tggtagtacc agtgctggtg gtagtgctgg
     8581 tggtagtgct ggtggtagtg ctggtggtag tgctggtggt tccggcagtg ctggtggtag
     8641 tgctggtggt agtaccagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     8701 tggtagtgct ggtggttccg gaatggtgag tgtgattaaa ccagagatga agatgaggta
     8761 ctacatggac ggctccgtca atgggcatga gttcacaatt gaaggtgaag gcacaggcag
     8821 accttacgag ggacatcaag agatgacact acgcgtcaca atggccgagg gcgggccaat
     8881 gcctttcgcg tttgacttag tgtcacacgt gttctgttac ggccacagag tatttactaa
     8941 atatccagaa gagataccag actatttcaa acaagcattt cctgaaggcc tgtcatggga
     9001 aaggtcgttg gagttcgaag atggtgggtc cgcttcagtc agtgcgcata taagccttag
     9061 aggaaacacc ttctaccaca aatccaaatt tactggggtt aactttcctg ccgatggtcc
     9121 tatcatgcaa aaccaaagtg ttgattggga gccatcaacc gagaaaatta ctgccagcga
     9181 cggagttctg aagggtgatg ttacgatgta cctaaaactt gaaggaggcg gcaatcacaa
     9241 atgccaattc aagactactt acaaggcggc aaaagagatt cttgaaatgc caggagacca
     9301 ttacatcggc catcgcctcg tcaggaaaac cgaaggcaac attactgagc aggtagaaga
     9361 tgcagtagct cattccgcgg ccgcattgag gcgcgcgacg ctggttgact ctagactgca
     9421 gctgcctcct ctggaacgcc tgactctgga ttaagtcgac ggatcctcga taatcaacct
     9481 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     9541 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     9601 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     9661 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     9721 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9781 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9841 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     9901 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9961 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    10021 cctcagacga gtcggatctc cctttgggcc gcctccccgc atctcgacaa aggatccaat
    10081 ggaggccgcc accgcggtgg agctcgaatt aattcatcga tgatgatcca gacatgataa
    10141 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
    10201 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
    10261 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
    10321 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatct
    10381 ttcttgcttt tacttttact tccttaatac tcaagtacaa ttttaatgga gtactttttt
    10441 acttttactc aagtaagatt ctagccagat acttttactt ttaattgagt aaaattttcc
    10501 ctaagtactt gtactttcac ttgagtaaaa tttttgagta ctttttacac ctctgtcaag
    10561 aaccatatgc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
    10621 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    10681 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    10741 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
    10801 gcgggtgtgg tggttacgcg cagcgtgacc accacacccg ccgcgcccta gcgcccgctc
    10861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    10921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    10981 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    11041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    11101 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    11161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    11221 caatttaggt gg
//
