LOCUS       sequence-322516-        8776 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express HA- and Clover-tagged HIF-2alpha (with a site-directed 
            mutation Ser581Ala) plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8776)
  TITLE     See lab plasmids
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HA-Clover-HIF-2alpha S581A
FEATURES             Location/Qualifiers
     source          1..8776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             906..932
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             906..932
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             912..938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             join(945..947,948..950,951..1661)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(990..1011)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1598..1619
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4331..4349)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4332..4349)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4369..4386)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4375..4599
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4645..5073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4732..4751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4942..4963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5082..5102)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5087..5416
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5267..5402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5329..5348
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5483..6277
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5537..5556)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6147..6166
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6451..6572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6488..6507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6542..6561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6621..6637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6621..6637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6634..6656)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6645..6661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6669..6675,6676..6693,6694..6699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6714..6735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6852..6869)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7023..7611)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7103..7122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7782..8573,8574..8642))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8405..8424
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8643..8747)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttacca
      901 tggcctaccc ctacgacgtg cccgactacg cctccctcgg atccatggtg agcaagggcg
      961 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1021 acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     1081 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     1141 gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1201 agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag gacgacggta
     1261 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1321 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1381 tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc aaggctaact
     1441 tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1501 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agccatcagt
     1561 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1621 ccgccgccgg gattacacat ggcatggacg agctgtacaa gggatccatg acagctgaca
     1681 aggagaagaa aaggagtagc tcggagagga ggaaggagaa gtcccgggat gctgcgcggt
     1741 gccggcggag caaggagacg gaggtgttct atgagctggc ccatgagctg cctctgcccc
     1801 acagtgtgag ctcccatctg gacaaggcct ccatcatgcg actggcaatc agcttcctgc
     1861 gaacacacaa gctcctctcc tcagtttgct ctgaaaacga gtccgaagcc gaagctgacc
     1921 agcagatgga caacttgtac ctgaaagcct tggagggttt cattgccgtg gtgacccaag
     1981 atggcgacat gatctttctg tcagaaaaca tcagcaagtt catgggactt acacaggtgg
     2041 agctaacagg acatagtatc tttgacttca ctcatccctg cgaccatgag gagattcgtg
     2101 agaacctgag tctcaaaaat ggctctggtt ttgggaaaaa aagcaaagac atgtccacag
     2161 agcgggactt cttcatgagg atgaagtgca cggtcaccaa cagaggccgt actgtcaacc
     2221 tcaagtcagc cacctggaag gtcttgcact gcacgggcca ggtgaaagtc tacaacaact
     2281 gccctcctca caatagtctg tgtggctaca aggagcccct gctgtcctgc ctcatcatca
     2341 tgtgtgaacc aatccagcac ccatcccaca tggacatccc cctggatagc aagaccttcc
     2401 tgagccgcca cagcatggac atgaagttca cctactgtga tgacagaatc acagaactga
     2461 ttggttacca ccctgaggag ctgcttggcc gctcagccta tgaattctac catgcgctag
     2521 actccgagaa catgaccaag agtcaccaga acttgtgcac caagggtcag gtagtaagtg
     2581 gccagtaccg gatgctcgca aagcatgggg gctacgtgtg gctggagacc caggggacgg
     2641 tcatctacaa ccctcgcaac ctgcagcccc agtgcatcat gtgtgtcaac tacgtcctga
     2701 gtgagattga gaagaatgac gtggtgttct ccatggacca gactgaatcc ctgttcaagc
     2761 cccacctgat ggccatgaac agcatctttg atagcagtgg caagggggct gtgtctgaga
     2821 agagtaactt cctattcacc aagctaaagg aggagcccga ggagctggcc cagctggctc
     2881 ccaccccagg agacgccatc atctctctgg atttcgggaa tcagaacttc gaggagtcct
     2941 cagcctatgg caaggccatc ctgcccccga gccagccatg ggccacggag ttgaggagcc
     3001 acagcaccca gagcgaggct gggagcctgc ctgccttcac cgtgccccag gcagctgccc
     3061 cgggcagcac cacccccagt gccaccagca gcagcagcag ctgctccacg cccaatagcc
     3121 ctgaagacta ttacacatct ttggataacg acctgaagat tgaagtgatt gagaagctct
     3181 tcgccatgga cacagaggcc aaggaccaat gcagtaccca gacggatttc aatgagctgg
     3241 acttggagac actggcaccc tatatcccca tggacgggga agacttccag ctaagcccca
     3301 tctgccccga ggagcggctc ttggcggaga acccacagtc caccccccag cactgcttca
     3361 gtgccatgac aaacatcttc cagccactgg cccctgtagc cccgcacgct cccttcctcc
     3421 tggacaagtt tcagcagcag ctggagagca agaagacaga gcccgagcac cggcccatgt
     3481 cctccatctt ctttgatgcc ggaagcaaag catccctgcc accgtgctgt ggccaggcca
     3541 gcacccctct ctcttccatg gggggcagat ccaataccca gtggccccca gatccaccat
     3601 tacattttgg gcccacaaag tgggccgtcg gggatcagcg cacagagttc ttgggagcag
     3661 cgccgttggg gccccctgtc tctccacccc atgtctccac cttcaagaca aggtctgcaa
     3721 agggttttgg ggctcgaggc ccagacgtgc tgagtccggc catggtagcc ctctccaaca
     3781 agctgaagct gaagcgacag ctggagtatg aagagcaagc cttccaggac ctgagcgggg
     3841 gggacccacc tggtggcagc acctcacatt tgatgtggaa acggatgaag aacctcaggg
     3901 gtgggagctg ccctttgatg ccggacaagc cactgagcgc aaatgtaccc aatgataagt
     3961 tcacccaaaa ccccatgagg ggcctgggcc atcccctgag acatctgccg ctgccacagc
     4021 ctccatctgc catcagtccc ggggagaaca gcaagagcag gttcccccca cagtgctacg
     4081 ccacccagta ccaggactac agcctgtcgt cagcccacaa ggtgtcaggc atggcaagcc
     4141 ggctgctcgg gccctcattt gagtcctacc tgctgcccga actgaccaga tatgactgtg
     4201 aggtgaacgt gcccgtgctg ggaagctcca cgctcctgca aggaggggac ctcctcagag
     4261 ccctggacca ggccacctga gatatccatc acactggcgg ccgctcgagc atgcatctag
     4321 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     4381 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4441 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4501 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4561 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4621 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4681 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4741 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4801 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4861 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4921 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4981 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5041 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5161 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5221 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5281 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5341 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5401 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5461 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5521 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5581 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5641 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5701 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5761 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5821 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5881 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5941 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6001 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6061 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6121 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6181 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6241 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6301 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6361 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6421 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6541 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6601 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6661 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6721 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6781 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7681 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7741 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7801 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7861 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7921 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7981 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8041 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8101 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8161 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8221 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8281 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8341 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8401 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8461 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8521 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8581 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8641 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8701 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8761 aaagtgccac ctgacg
//
