LOCUS       sequence-32357-e         945 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Hardin JW, Hu YX, McKay DB
  TITLE     Structure of the RNA Binding Domain of a DEAD-Box Helicase Bound to 
            Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an 
            RNA Recognition Motif.
  JOURNAL   J Mol Biol. 2010 Jul 29. ():.
  PUBMED    20673833
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2010 Jul 29. ():."
COMMENT     Sequence Label: 23SrRNA.RAV12(wt).2508-2580.G2530AAAC
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(44..91)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(335..353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(360..377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(360..376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(369..391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(585..604)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     704..726
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(764..782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catttcacac aggaacagct atgaccatga ttacgccaag cttcaaaaaa cccctcaaga
       61 cccgtttaga ggccccaagg ggttatgcta gggatcctgc ccagggctgc tacccgaagg
      121 tcctgacccg atgcacagga aaaaactgcc cagggctgct acctttcagt cctgacccga
      181 tgcacagtct agaggtccca ttcgccatgc cgaagcatgt tgcccaccgg ccgccagcga
      241 ggaggctggg accatggccg gctagctcgc gtaccacttt aaatggcgaa cagccatacc
      301 cttgggaccg tttccagccc caggatgtga tgagcctata gtgagtcgta ttagaattca
      361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      481 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
      541 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
      601 gccgcatagt taagccagcc ccgacacccg ccaacaccca ctgacgcgcc ctgacgggct
      661 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
      721 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
      781 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaa
//
