LOCUS       sequence-32361-e         916 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Hardin JW, Hu YX, McKay DB
  TITLE     Structure of the RNA Binding Domain of a DEAD-Box Helicase Bound to 
            Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an 
            RNA Recognition Motif.
  JOURNAL   J Mol Biol. 2010 Jul 29. ():.
  PUBMED    20673833
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2010 Jul 29. ():."
COMMENT     Sequence Label: 23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11..27
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(46..93)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(333..351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(358..375)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(358..374)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(367..389)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(583..602)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     702..724
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(762..780)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acatttcaca caggaaacag ctatgaccat gattacgcca agcttcaaaa aacccctcaa
       61 gacccgttta gaggccccaa ggggttatgc tagggatcct gcccagggct gctacccgaa
      121 ggtcctgacc cgatgcacag gaaaaaactg cccagggctg ctacctttca gtcctgaccc
      181 gatgcacagt ctagaggtcc cattcgccat gccgaagcat gttgcccacc ggccgccagc
      241 gaggaggctg ggaccatggc cggctgactc atcactttaa atggcgaaca gccataccct
      301 tgggaccgtt tccagcccca ggatgtaatg agcctatagt gagtcgtatt agaattcact
      361 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      421 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      481 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      541 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      601 cgcatagtta agccagcccc gacacccgcc aacacccact gacgcgccct gacgggcttg
      661 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      721 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
      781 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      841 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      901 catgagacaa taaccc
//
