LOCUS       sequence-323936-        2300 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.296 L3S3P11
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             160..951
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1122..1710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1611..1630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1864..1881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2048..2066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2134..2238
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
       61 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
      121 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
      181 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
      241 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
      301 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
      361 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
      421 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
      481 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
      541 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
      601 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
      661 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
      721 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
      781 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
      841 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
      901 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
      961 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1021 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1261 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1501 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1741 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1801 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1861 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     1921 gcgcgttggc cgattcatta atcactctgt ggtctcagct tccaattatt gaacaccctt
     1981 cggggtgttt ttttgtttct ggtctccccg cttgagacca cgaagtggct cttcagtgga
     2041 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     2101 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2161 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2221 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     2281 ggtggtaacg gcgcagtggc
//
