LOCUS       sequence-324194-       10177 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses JIP3 with a GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, 
            Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, Li Y, 
            Cookson MR
  TITLE     LRRK2 mediates tubulation and vesicle sorting from lysosomes.
  JOURNAL   Sci Adv. 2020 Nov 11;6(46). pii: 6/46/eabb2454. doi: 
            10.1126/sciadv.abb2454. Print 2020 Nov.
  PUBMED    33177079
REFERENCE   2  (bases 1 to 10177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-11-11"; volume: "6(46). pii: 6/46/eabb2454. doi"; pages:
            " 10.1126/sciadv.abb2454. Print 2020 Nov"
COMMENT     Sequence Label: GFP-JIP3
FEATURES             Location/Qualifiers
     source          1..10177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(905..907,908..910,911..1621)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(938..966)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1561..1581
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    1643..1667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5712..5736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5768..5785)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5774..5998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6044..6472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6131..6150)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6341..6362
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6481..6501)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6486..6815
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6666..6801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6728..6747
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6882..7676
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6936..6955)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7546..7565
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7850..7971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7887..7906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7941..7960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8020..8036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8020..8036)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8033..8055)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8044..8060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8068..8074,8075..8092,8093..8098))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8113..8134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8251..8268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8422..9010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8502..8521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9181..9972,9973..10041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9804..9823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10042..10146)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac
     1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
     1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat
     1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     1621 aagcggttcc ggaccggatc aaacaagttt gtacaaaaaa gcaggcttga aggaattcgg
     1681 taccatgatg gagatccaga tggacgaggg cggcggcgtg gtggtgtacc aggacgacta
     1741 ctgctccggc tcggtgatgt cggagcgggt gtcgggcctg gcgggctcca tctaccgcga
     1801 gttcgagcgc ctcatccact gctacgacga ggaggtggtc aaggagctca tgccgctggt
     1861 ggtgaacgtg ctggagaacc tagactcggt gctcagcgag aaccaggagc acgaggtgga
     1921 gctggagctg ctgcgcgagg acaacgagca gctgctcacc cagtacgagc gtgagaaggc
     1981 gctgcgcagg caggcggagg agaaattcat tgagtttgaa gatgctctgg aacaagagaa
     2041 gaaagagctg caaatccagg tggagcacta cgagttccag acgcgccagc tggagctgaa
     2101 ggccaagaac tatgccgatc agatttcccg gttggaggag cgggagtcgg agatgaagaa
     2161 ggagtacaat gccctgcacc agcggcacac agagatgata cagacctacg tggagcacat
     2221 tgagaggtcc aagatgcagc aggtcggagg aaacagccag accgagagca gcctgccggg
     2281 gcggaggaag gagcgcccca cctccctgaa cgtgttcccc ctggctgacg gcacggtacg
     2341 tgcacagatc gggggcaagc tcgtgcctgc gggggaccac tggcacctga gtgacctcgg
     2401 ccagctgcag tccagctcca gctaccagtg tccacaggat gaaatgtccg agtcaggcca
     2461 gtcctcggcg gccgccacac ccagcaccac aggcaccaag tccaacacgc ccacatcctc
     2521 cgtgccctcg gccgccgtca cacccctcaa cgagagcctg cagcccctgg gggactatgg
     2581 cgtgggctcc aagaacagca agcgtgcccg ggagaagcgc gacagccgca acatggaagt
     2641 acaggtcacc caggagatgc gcaacgtcag tataggcatg ggcagcagtg acgagtggtc
     2701 tgatgttcaa gacattattg actccacgcc agagctggac atgtgtccag agacccgcct
     2761 ggaccgcaca ggaagcagcc caacccaggg catcgtgaac aaagctttcg gcatcaacac
     2821 cgactccctg taccatgagc tgtcgacggc agggtctgag gtcatcgggg atgtggacga
     2881 aggggccgac ctcctagggg agttctcagt gcgcgatgat ttctttggaa tgggcaaaga
     2941 agtggggaat ctgctactgg aaaactcaca gcttctggaa accaaaaacg ccttgaatgt
     3001 ggtgaagaat gacctgattg ccaaggtcga ccagctgtcc ggggagcagg aggtgctgag
     3061 gggcgagttg gaggctgcta agcaggccaa agtcaagctg gaaaaccgta tcaaggagct
     3121 ggaagaggaa ctgaaaagag tgaagtccga ggccatcatc gcccgccgtg aacccaaaga
     3181 agaggcggag gatgtaagca gctatctctg tacagaatcg gacaaaatcc ccatggccca
     3241 gcgccgccgc ttcacgcggg tggagatggc ccgtgtgctc atggagcgga accagtacaa
     3301 ggagcggctg atggagctgc aggaggctgt gcggtggact gagatgatca gagcgtcccg
     3361 agagcaccca tccgtccagg agaagaagaa gtcgaccatc tggcagttct tcagccgcct
     3421 cttcagctct tcctccagcc cccctccggc caagcgcccc tatccctcgg tgaacatcca
     3481 ctacaagtca cccaccactg ccggcttcag ccagcgccgc aaccatgcca tgtgcccgat
     3541 ctcggcaggc agccggcccc tggaattctt ccctgacgac gactgcacgt cctccgcccg
     3601 tcgagagcag aagcgcgagc agtaccgcca ggtgcgtgag cacgtgcgta acgacgacgg
     3661 ccgtctgcag gcctgcggct ggagcctgcc cgccaagtac aagcagctga gtcccaacgg
     3721 gggccaggag gacacgcgga tgaagaacgt gccggtgccg gtgtactgcc gccctctggt
     3781 ggagaaggac cccaccatga agctgtggtg tgccgcgggc gtcaacctga gcgggtggag
     3841 gcccaatgag gacgacgctg ggaatggagt caagccagcg ccaggccgcg atcccctgac
     3901 ctgcgaccga gaaggagacg gcgagcccaa gagcgcccac acgtctcccg agaagaagaa
     3961 ggccaaggag ctccctgaaa tggacgccac ctccagccgg gtgtggatcc tgaccagcac
     4021 cctgaccacc agcaaggtgg tgatcatcga cgccaaccag ccgggcacgg tggtggacca
     4081 gttcaccgtc tgcaacgcgc acgtgctgtg catctccagc atccccgcgg ccagcgacag
     4141 cgactaccct cccggggaga tgttcctgga cagcgacgtg aacccagagg acccgggcgc
     4201 agatggcgtg ctggccggta tcaccctggt gggctgtgcc acccgctgca acgtgccgcg
     4261 gagcaactgc tcctcccgag gggacacccc agtgctagac aaggggcagg gggaggtggc
     4321 caccatcgcc aacgggaagg tcaacccgtc ccagtccaca gaggaggcca cagaggccac
     4381 ggaggtgcca gaccctgggc ccagcgagcc agagacagcc acattgcggc ccgggcctct
     4441 cacagagcac gtcttcactg acccagcccc gaccccgtcc tctggccccc agcctggcag
     4501 cgagaacggg ccagagcctg acagcagcag cacacggcca gagccagagc ccagcgggga
     4561 ccccacggga gcaggcagca gtgctgcacc caccatgtgg ctgggagccc agaacggctg
     4621 gctctatgtg cactcggctg tggccaactg gaagaagtgc ctgcactcca tcaagctgaa
     4681 ggattctgtg ctgagcctgg tgcatgtcaa aggccgtgtg ctggtggctc tggcggacgg
     4741 gaccctggcc atcttccacc gtggtgaaga tggccagtgg gatctgagca actatcacct
     4801 aatggacctg ggccacccgc accactccat ccgctgcatg gctgttgtgt acgaccgcgt
     4861 gtggtgtggc tacaagaaca aggtgcacgt catccagccc aagaccatgc agatagagaa
     4921 gtcatttgac gcccacccgc ggcgggagag ccaggtgcgg cagctggcgt ggatcggcga
     4981 tggcgtatgg gtgtccatcc gcctggactc caccctgagg ctctaccatg cacacacgca
     5041 ccagcatcta caggacgtgg acattgagcc ctacgtcagc aagatgctag gcactggcaa
     5101 gctgggtttc tccttcgtac gcatcacggc cctgcttgtc gcgggcagcc ggctctgggt
     5161 gggcaccggc aacggagtgg tcatctccat ccccctgaca gagactgtgg tcctgcaccg
     5221 aggccagctc ctggggctcc gagccaataa gacatccccc acctctgggg agggcgcccg
     5281 tcccgggggc atcatccacg tgtatggcga tgacagcagt gacagggcgg ccagcagctt
     5341 catcccctac tgctccatgg cccaggccca gctatgcttc catgggcacc gcgatgccgt
     5401 gaagttcttt gtctcggtgc cagggaacgt gctggccacc ctgaatggca gtgtgctgga
     5461 cagcccagcc gagggccctg ggccagctgc ccctgcctcg gaggtcgagg gccagaagct
     5521 gcggaacgtg ctggtgctga gcggcgggga gggctacatc gacttccgca ttggagacgg
     5581 agaggacgac gagacggagg agggcgcagg ggacatgagc caggtgaagc ccgtgctgtc
     5641 caaggcagag cgcagtcaca tcatcgtgtg gcaggtgtcc tacacccccg agtagctcga
     5701 gtgcggccgc aacccagctt tcttgtacaa agtggtgata attaattaag ataaacccgc
     5761 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5821 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5881 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5941 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6001 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6061 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6241 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6361 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6421 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     6481 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6541 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6601 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6661 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6721 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6781 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6841 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6901 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6961 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7021 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     7081 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7141 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7201 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7261 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7321 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7381 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7441 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7501 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7561 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7621 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7681 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7741 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7801 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7981 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8041 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8101 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8221 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8821 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10141 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
