LOCUS       sequence-324195-       14572 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Second CCM operon cloned from H. neapolitanus; pFA expressing the 
            remaining 11 genes in the H. neapolitanus CCM cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 14572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCCM
FEATURES             Location/Qualifiers
     source          1..14572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..44)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(161..706)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(241..260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(820..914)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(935..1594)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1509..1528
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1595..1697)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1763..1781
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1802..1820,1821..1826,1827..1843,1844..1849,
                     1850..1875)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1802..1820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1827..1845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1885..1896
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      13566..13652
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(13825..14448)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     14356..14375
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(14449..14553)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctagacctag ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
       61 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
      121 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
      181 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
      241 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
      301 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
      361 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
      421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
      481 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
      541 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
      601 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
      661 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
      721 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
      781 agtcagcccc atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc
      841 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      901 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
      961 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1021 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1081 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1141 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1201 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1261 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1321 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt
     1381 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1441 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1501 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1561 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1621 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1681 ctcttacgtg ccgatcaacg tctcattttc gccagatatc gacgtctaag aaaccattat
     1741 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga
     1801 gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg agcacatcag
     1861 caggacgcac tgaccgaatt cattaaagag gagaaaggta ccatggcgat agaaaagcta
     1921 aatcagattg aggttgatgg ttgggaatgc cgtaaattac cacctcaatt aactcgccgc
     1981 ttcgattttg gaagttattc tcaaacgcgt tcatttttgg acgatttagc tgatttatct
     2041 gaggccattg gcaactatcc caacttgcat tttgccaaag aattcgtgac cgtcaccata
     2101 tctgctgcgg acgacggtcc attgcaagag tctgaggttg aatttgctac ccaagtcaat
     2161 gacttggccg ccaagtatag ctaacgccat ggccagcaac aaagccttta cattggctgt
     2221 cgccaacctc aaaggtggct gtggcaagac gaccatcagc acaaacatca gtgcgggctt
     2281 gacgcagcgt ggtcgtgttg gtctggtcga tgccgacccc caaggcgcac tcaagcattg
     2341 ggttgactgg ggaagcaaag aggcggacgc tcaagtcccc gtcttatact cagatcacac
     2401 cgatcccgtt caaaatctta aattggcgca accaaaccat gactttgtcg tcgtggattg
     2461 cccgccttcc ctagacatgg caattacctg tcaattgatg attgagtgcg attttatatt
     2521 aattcctgta ctgccctccc ctttagatct ttgggcgagc actcaaacaa tcgagatgat
     2581 tgaaagcgca cgcaaaacca accccaagct aaaagctgcg ttggtactca atcaaacaga
     2641 gccgcgcagt gcaatgacac gagcaatgca aacaaccatt gagcgactag gggttcccgt
     2701 actcactacc tctgtgcgac gacgcgctgt gtaccgaaat gccgtagtgg aaggcgttag
     2761 cgttttccag ctcggagcaa gaggccgctc agcggctgat gaaatcaatc aaatccttaa
     2821 cgaggttatc ccgtcatgac taatttagaa gataaactga gtgcaagcat caagaccgag
     2881 aacaaggaca cgatagcgcc tgcgtcaacg ccagccaaac cagccgcgcg ctcggctgca
     2941 cccgaaacca ccaagcctgc atcgacaggg gccaaacctg ccacacggac ggctgcgaaa
     3001 aagcctgtag cgcgtgcgac gacaacaaaa cctgctgtat caaaatccag caagccatcg
     3061 gctgatgata ttcaggttcc ggcacacgac acatcgacgc atcctcgccg tgtatggcca
     3121 gattgatttt gtccgaacat cgcgaaacta acgtcaaact atgaacccag cgactgaaga
     3181 atatcaggct ctttttgaag acttgggcga accgatcgct gaagtccttg agacccattg
     3241 gattgagctt tcgcgattgt tcagcccgat gggtttcaag gcttttctag aaagcgtttc
     3301 caatcttaag gctttgggac gcggtgatga gttagttatc tcgtttattg aatcggcagg
     3361 tcctgttaca aggtcaattg gtgaaacagc agttttcgaa ctgctgtctt ccgttattaa
     3421 aatgttctcc aaggccagtg ctgaagtttt agcaatggtt ttcaaatcgg cacctgttgc
     3481 tgtcagtcgg ctcggtgagg ctgaactgtt cgttgcctat ctttctttac tggattacat
     3541 agcggccaaa gccccccgag ccttacgtcc acttcttggt caaatagagc aactgcttgg
     3601 tgtgctgacg cttgggggat tgcggcgttg gatcacttgg gggattcaag cgcaccgaac
     3661 cgattttgcc gcccaagagg cctactttag tcttgcaacg cctgaatccc gtgctgtgtt
     3721 acagcgcgag cgaaggggtg tattgtttgt tgatgtacaa cgccgcttgg tgatgtattt
     3781 acgcgcactg tgggggcgtg atttttttct acgccccact tcgggtgatt tcgaaagccg
     3841 tgcgggatac cgtccgttca ttgatgaact atttatcaat ttacccgacg cgtatgatga
     3901 ttacgccgtt gaaggcgaac aaacagttga tggtctgact gtctatcgtg cggcagcggc
     3961 tcatgccgca gcacatcaaa tttattcgcg tctcgactcc gatgcgaaga acgcatcccc
     4021 actggaccgc gcgaccattg gtttatttga agacgcgcgt gtcgaaaaac tcgcaatggc
     4081 cgatttccct ggattggttc ccctgtggag gaatctcaac caactgacgg ttgctgccct
     4141 ccccgctgac gcacgaacag gagagaatcc aactcttggc gcacttttcg accggatagc
     4201 gctggcacta tgcgatgaga attcatcgga tacccacccg cttgtcgaat ggtcgcgtga
     4261 ggcgtttaaa gcagcacttg atcgtctgga agatcaggag atggcgcgag aactgggcat
     4321 cgctctagcc gaaaagatcc agcagcaaaa cctggtattt caaagccgta ccgatatccc
     4381 aagtagcccg taccgtgatg acaataccta tttgtggcaa acaccggaaa caggcaagag
     4441 caatgcacat accgcggcca gcattcagga aaagcgcaag tacgttagcg tgatgcagat
     4501 gctgcacgcc ttggatgtgg agtacgccac ggcggaaagc gcggatgaaa tatgggtgct
     4561 tgccaccgaa ctttttgatg acgatggcac gacgttcaat gagctttatg gcacgccacc
     4621 gactgcttcg cctcgatatt atcccgaatg ggattaccaa tcacagcttg agcgaccatt
     4681 gtggacgacc ctgatggaga agaaacccaa attgggcgat cctgcggtgg ttgataatat
     4741 tttgttcgag cacgccccaa tggtacggcg tttgaagtac ctgatcgaag gcttacagcc
     4801 acaaggtgtc gtgcgacaga aaaaaatcga ggatggtgat gaagtcgatc tcaatgcagc
     4861 cattggcgca atgatcgata ttcgaatgaa tcgtcaaccg gacccgcgca ttggcattcg
     4921 tcgacgtatc catgtgcgtg atctatccgt gattttgctc attgatctct cagaatcaac
     4981 caatgatctc gtgcaaaccg gtgaagaaga aaaaaccgta cttgatatgg ctagagaggc
     5041 agcagcatta ctcggtgatg cattgaatcg cattggtgat ccctacgcta ttcatggttt
     5101 tgattccaat gggcgcagcg atgttgagta tttccgcttc aaagactttg atgacgcctt
     5161 cgatgacctc gccaaggctc gtctggccgg aatgacgggg caactctcca cacgcatggg
     5221 cactgccttg cgacacgcgg gccatttgtt ggattcacgg cccagccaga agaaacttat
     5281 tctgttactt accgatggcg agccttccga taatgatgtg cgcgaccccc aatatctacg
     5341 ccaagacacc aagcgcgttg ttgaggaatt agggaaacgg ggcatcaata cgttctgcgt
     5401 caccctcgat ccaagagccg atgattatgt cgaacgaatt tttggcccga aacactacat
     5461 gatcctcgac catgtagctc gactgcctga gaaactaccg gcactttatt tgtcgatgac
     5521 gcgatagaac tttccgtccg agcttcagac aagaaacaaa tctcatgaag ctgttcaaat
     5581 tgctgctcgg gatacgagga caatgcgcct gaaacccgat taattttaat ttgcgtatac
     5641 gcaagataac tacccggcca aggccggtct gtgatggaga aagcgcatga tgaacctgca
     5701 atggttaatt ccattgttac ccttactgtc cgccctactg gtgcaattgt ttgccgctcg
     5761 actcggtaaa cgtgttgccc acctgagcgt ggcgttggga acactgaccg tcattgttgc
     5821 cgcatatcag ctcgtagcgt atatcggtac cgatgcgagc ccaagctggc attctctggg
     5881 cacgatgtgg ggcagtctgt acgtcgatcc gctgagcagc atcatgtcgc tggttgtagc
     5941 gggcatcagc ctgattgttc atgtctattc aatccgctac atgaccgaag aaccgggcta
     6001 tccccggttc ttcttgctgc tggatttgat gaccgcttcc attttgctta tggtcgccgc
     6061 cggtgacttg gtcacattgc tgatcgcctg gcacatgatc ggcatcatgc tctactttct
     6121 tttgggtcag aatacagaat catggccgag ccagcgctac gccttttgga cgtttatcac
     6181 gtaccggttg ggggacttgc cattggtgct cgccgccgtg ctgctttatc aaacctatgg
     6241 tgcgattgac ttccccacct tattttcccg tatcgcggcc gatccaaacg ccaccatcat
     6301 ggggctgccc acggcgataa cggctgcttt tttggtggcg ctctctgcct ttgcaaaatc
     6361 cgcccaattc ccgctacata cctggctccc ctataccatg gagggcccga cgccggtttc
     6421 agctttgatg catgcgggga ttgtcaacgc cggtggtttt atcatcaacc gcttcgcgcc
     6481 tgtttttgtt cacagtgatg gcgtactgca catgctgttt gtcgtgggtc tgattaccgc
     6541 gctggtggga tcggtgctga tgttgacgca aaacgacatc aaaaaatcac tgggttattc
     6601 caccatgggt caaatgggct tcatggtcat ggagtgtggt cttggcgctt tttctctggc
     6661 cgtgttccat ctcattgcgc atggtctgtt caaaggcacc atgttcctcg gttcgggcag
     6721 catgattcat gaagcacgca agcacgacgg cgtgccacat aaccctctgc acaccttctt
     6781 ggttgaacga aagtctgcat cattaaaatt gccttggtta tttatcggct tggctacgct
     6841 ggtcgtacct ctgttcattc tggttattgc acactggttc gtggcaccgg attttttcga
     6901 aaaacaaggt gccatcgtgc tgctgttctt cggctggatt acgggggttc aggttctttt
     6961 tgccacacat catctggatg ccaataaccc aattcgcatg atgatgatga ttttattgtc
     7021 gtttaccttg attgtggtgg gctacacctt cattggtcat gctttcgaaa acttccttta
     7081 ccccgaagaa gccttccgta atgcgctgta ccatgcagca ggcatcgata aactgacgtt
     7141 cgacggactt gttttcttgc tcgctttgat tgtggtggca ggctggttta gcagctattt
     7201 ggccagtcgt gagaaatcgg ttttcggtga ccggttcgga gcggttcgcc tcactctcta
     7261 ctcactgatc tcgcgtgaat tttatgtagc cgatctctac gaccggatgg cgcactggct
     7321 gcttgatgca tccaaacgct tcaatgtctg gatgaggtgg tattgatatg tcctttttct
     7381 tgatgttact ggccgggttc tttctgccct tgttcccctt gagcatggta ttcaatgccc
     7441 tgctcggccg aatcaaaaac cccatcgcgc gggtactgct gatttttatc tggcctcaaa
     7501 tcggcgtttt cttgcttgca tgggctggtt tgccgctgga ttccggattc ttcgttggct
     7561 gggcagtgct tacggcagtc ttctatgcct ggcgattgct gacggtgcgt gtgctctccc
     7621 agtgggccgg gatgctcgcc acgtccgcct gggcgctggc ttggattatc gccgccgatc
     7681 ttccagccag tagcgaactg cacagtttcg ccctgtggat gtccgttgcg ccggctttgc
     7741 tgatcttcat tgccattgca ctgcaagaac gttttggcgc tgcttacagt ggtttagttg
     7801 tggggttggc gcgggcttta ccgcgcttat ccagcgtcat tgtcatcgtg gtgttggccg
     7861 caatggcact ccctattttc cccagttttt ttgcaatgtt ggcattgctg gcgcatagct
     7921 ccatcttatt cgctctgccg attttgctta cctggttgct ttggagttgg gcaggcacgc
     7981 ggttattgaa tgggtttgtt ttcgggccac gcgccgagac agatgccgcc gatctctctt
     8041 caaccacaac atggggcttt gttggcctga tagcaacgat tgttgtgatt aatctgattt
     8101 ggacaggagc ttgatcccat gagcaaactc cctttgggta aacgcctgaa aattcgttcc
     8161 atggtgcaca tggctgccga gccaatcccc aatttctggc ctatgcggac gttcattcac
     8221 cataacccgc tccatggtct ggagcatctg cccttcgaac aggcggttcg ccagggtgaa
     8281 aaactctttc acgcacgggg atttttgccg cgtgaggatt accagcgcta tcacaaggaa
     8341 ggccgagttg atcaaaacag cataaagcgt gacatagccg attttatttc aaaacaagaa
     8401 acgctcaacg gtttggattt ggcatcgtta cttagcgact tgatgtgttc ggttaagaac
     8461 aaagtaacta gaacgcgcgc gctcgccgat catgatgatg tgttccaagc cttgcacggg
     8521 aaacaactgg aaaatgcaga ggcgctcgat cttaaagcgc taactcaacg cttatgtgcg
     8581 cagtttgcac cagagcgccc cctgtacgaa gccatcgatt tgctgttcgg cacgcaaatg
     8641 ggcaccacac tcgatgaatt ggtaatcaaa agctgtctcg actttttcga tgaaggccaa
     8701 tcaaccatcc aaatgcccgg ccgccaccaa ggattgttcg cagcttggac ggccctggca
     8761 aaacgtaatt tacgcctgtt tttacgtggc atgcatatca aacagatcct cgatcaggac
     8821 gatacgccag agggcatcat cgcctacatt ctcgacgaac tgggcattga ggaggctcac
     8881 tgggatgggc tgattacccg cgaactgact cgtttgcatg gctgggcagg ttttattcgc
     8941 tggcgctcct cttccaagca ctactattgg gccgagcagt acccggggga tctcatcgat
     9001 ttcctggcca tccggctcgt tttaggcttg gccttgatcc gtgaacatag ccgtcaaaag
     9061 cgcacaccga tgacagtcaa agtgctgcaa gaatatatcg aagggcacac cgccgaatgc
     9121 tatctgcgtc aagcctatta cggtggctgc atattgcccg cgtttgctca tgatgttgat
     9181 gatgcgctgt cgcataaaaa gcctcaaagg atcaacaaca ttcttccggg ctacctgcgc
     9241 caacaacgcc aattcgaggc aacacgacaa gctgatgcgc ttcgtgattt agccagcaaa
     9301 gcggggcaaa ccgatgccct tatggcgctg aatgcgcctc aaatcaagca actcatgaca
     9361 cttatcgagg cgtttgaaaa cgaagaaggc atgatctggc ttcgtgcgat ggaatcggtc
     9421 tatcgacggg aaatcatcaa ccagattcaa ctgtatgcac cgcataaaaa agaaaaacgg
     9481 ccctttgccc aggcattgtt ctgtatcgat gtgcgctccg agccgatacg ccgtaatctg
     9541 gaaacggtag gcgagtatca aacctatggt atcgccgggt tttttggtgt tccggtaagc
     9601 tatattggcc ttggcaaggg cagtgaagtt aatctttgcc cggtggtcat tacccctaaa
     9661 aatctggtgc ttgaagtgcc cgtgggtgcc acaagcattg aaacagactt ttattcttcc
     9721 gccgaccatg tgctacatga gatgaaaagc tcgatccttt caccctactt cacggttgaa
     9781 gcggccggtt tgctgtttgg tttcgacatg atcggcaaaa ccattgcccc gcgacgctac
     9841 acccaaatac gcaatcatat cgaaccaaaa gcacaggcaa ctcgtttgct ggtggataaa
     9901 ctcacccgcg aacaagccga ctcaatcgtc cgttcgctgc aacgcgccat gattgtgcgc
     9961 gccattcatc aggaatttgg catcgaacgc gaagcagtca ccgatgccat gatccgcgaa
    10021 ctgcgcgaag cggccatgga taactatcac gaacagaccg aattcgcgcg ccgtttcgcc
    10081 ttgagcccaa cggccgaaac tcagtttatc gcagggctga aaaaggacta taaaatcaat
    10141 cgctcatttg tttccatgca aatggaacgc ttggccagga ttggttttag cctcgatgaa
    10201 caggtgtttt atgttgacaa ggcactcaca tccatcggat tgaccgaaaa cttttcacgc
    10261 tttgttttat tggccggtca cggcagcact tccgacaata atccctacga atccgcgctt
    10321 gactgcggtg catgtggtgg tagtcatggg ctggtttctg cccgggtgct tgcccacatg
    10381 gccaataagc ctgaagtacg tcgcagactg gccaagcaag gcatccagat acctgaagat
    10441 acttggtttg tgtccgtcat gcacaacacc acaaccgatc aattgtcact gcaagacctt
    10501 gatttgcttc caaacagcca tcttgtttac ctcgaacgct tgcgtaacgg cttacgtgcg
    10561 gccacccgtt tgtcagcggc agaacgcttg cctgctctgc ttgatcatcc ttcgcccaac
    10621 atcgacacat tatcggcaca aaaacaaatt gagcggaatg ccagcgactg gacccaagtt
    10681 cggccagagt ggggcttggc gagaaatgcg agcgtcgtcg ccggcggccg acatttgacc
    10741 gagggtgcga acttaagcgg tcgaacgttt ttgcagtctt acgattatcg actcgatccc
    10801 aaaggccgcc accttgaaaa cattctcagc aacccgctaa ttatcggcca gtggatcaat
    10861 cttgagcatt atttctcagc ggtagataac gaacactttg gcagtggcag caaggcctat
    10921 cacaacgtcg taggtcgttt tggtgtggtt acgggtaatt taagtgactt gcgaacaggg
    10981 ttaccagcac agtcggtgct taaagatgga cgcccatacc acgagcccat ccgtctcttg
    11041 gcgattatcg aagcacccgc agcattcacc ctcgaagtag cgggtcgatt gcccaaggtg
    11101 atgtccctga ttaccaacgg ctggatcact gttgttgtcg ttgatccgga aacgggcgat
    11161 cgtctttttt atgatcgcgg cgaatggtac aatctcaaca atgatccgca gtacacgccc
    11221 tcggtcaaac ccttgcttga agaagaactc agcgcatgaa aaacctcaac cttacccaat
    11281 tgaaaaaact ggaaatcatc ctcgaaggtg aacatcaatc attcgccacg gatttactcg
    11341 accgagcagg cgtcaagggc tacaccattg tcggcaacct ttcaggcaaa ggggctcaag
    11401 gctttcatga aggccatctc atgtttaacg aagatgaagt gctgaacatg atcatcaccg
    11461 ccgttccgga agagctcgtt tctcccttgc tggaaggctt cgcccccttt ttcagcaaac
    11521 acagcggagt agtatttgtt tctgacattc aggtcagtcg tccggtcaag ttcaaaacag
    11581 acgctaaata aatacgattt aaatcagaaa gacggctaca tcgaccagaa caaggcaatt
    11641 taaatgacac aaaatgcaga tcaatatcgc gtacgcaaag agccctatta tcgagctgta
    11701 caagacgaaa tcgagttata tcgcgccggt tacgaagcac gcattcccat gatgctcaaa
    11761 ggcccgaccg gctgtggtaa atcacgtttt gtcgaacata tggcttggaa gctcaaccgc
    11821 cccttaatca ccatcgcgtg taatgaagac atgacggcat ccgatcttgt cggccgctat
    11881 ttgctcgata aagatggcac ccgttggcaa gatggcccgc ttacggtcgc tgcgcgcatt
    11941 ggtgccatct gctacctcga tgaaattgtt gaggcccgtc aggataccat cgtcgtcatc
    12001 catccattga ccgatcaccg tcgagtactg ccacttgaga aaaagggcga attggttgaa
    12061 gcgcatcccg atttccaaat cgtgatttct tacaatccgg gctatcaaag cctgatgaaa
    12121 gacctcaagc aatccaccaa gcaacgtttt ggtgcgcttg attttgacta ccctaaacca
    12181 gacattgaag ccgaaattgt gagccacgag gccggggttg ataaagacac tgctgaaaaa
    12241 ctggttcaga tcgcacaaaa agcacgtaac ctcaagggtc atggcctgga tgaaggcctc
    12301 tccacgcgtc tgttagtcta tgcaggcaaa ttgattgcca aaggcgttga tgcccgcgct
    12361 gcctgcacca tgacgcttgt caacccgatc acagatgatg tcgacatgcg cgatgcactc
    12421 gacaccgtcg tcaaaacgtt cttttaagcc ttccgtacca acaggtaata cgcacaacat
    12481 gaaacacacc agctatgtcg gtcttgatct tgacgcacaa ggcggcatga cgcacatcgg
    12541 aaaaattatc cgagatggtt ggctgtttgg ctttattcca gaaacggaaa cgggtaccgg
    12601 ctggacccac acacagattc aaatgctgta tgaaaaggtg tttgctgcct gggaaccgta
    12661 tgcacacatc ccgagccgct tgccacccga gcttaaagaa aagtacctaa gaattcagga
    12721 agatgcacta aaacgcgccc gggaaaatgg atgggacccg gaactgtccg atgatgatta
    12781 aaactccaga ccaaactgta tccgcccaaa tcaagataca agtgaaaatc aatgaaatca
    12841 gtgtgcatct tcagggaaga tgcgcatgaa caacattgat ttgcgcgtct attcgtttat
    12901 cgattcgctt caaccgcagc ttgcatccta tcttgcgaca tcatcgcaag gctttcttcc
    12961 cgttccgggt gatgcctgct tgtggattga agtcgcgccg ggcatggccg tccatcgcct
    13021 cagtgatatt gcgctaaagg ccacgaacgt tcgtctcggc gaacaggtag tcgagcgtgc
    13081 ttttggctcg atggaaattc attaccgaaa ccaaagcgac gttctcgcat ccggtgaggc
    13141 cgttttaaga gaaatcaatc acgcgcaaga agatcgtctg ccttgtcgca tcgcatggaa
    13201 agagatcatt cgagcgatta cccccgatca tgccaccttg atcaatcgcc agttgcgtaa
    13261 aggctctatg ctgttgccgg gcaaaagcat gttcatcctt gaaacagaac cggcaggtta
    13321 tattgttcag gctgccaacg aggccgagaa agcagctcat gtgactctga tcgatgtacg
    13381 tgcttttggt aactttggtc gcctgaccat gatgggcagc gaagcggaaa ccgaagaagc
    13441 catgcgggcg gctgaggcca caatcgcaag catcaacgcg cgtgcgcgtc gcgctgaagg
    13501 gttctaaaat gtaactgtat tcagcgatga cgaaattctt agctattgta atactctaga
    13561 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
    13621 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc gctagcagtt cgtagacgga
    13681 aacaaacgca gaatccaagc aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13741 actcttcctt tttcaatatt attgaagcat ttatcagggt tatgtccact gggttcgtgc
    13801 cttcatccgg atatccagga actgttaaga cccactttca catttaagtt gtttttctaa
    13861 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa
    13921 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc
    13981 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac
    14041 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa
    14101 ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc
    14161 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc
    14221 ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat
    14281 ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc
    14341 tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag
    14401 cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatac tcttcctttt
    14461 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    14521 tatttagaaa aataaacaaa taggggttcc gcgtttccct cgacaattcg cg
//
