LOCUS       sequence-324304-        9475 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Serves as control to pLV-PGK-dt/CBA-EGFP-106bT..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Choi SH, Reeves RE, Romano Ibarra GS, Lynch TJ, Shahin WS, Feng Z, 
            Gasser GN, Winter MC, Evans TIA, Liu X, Luo M, Zhang Y, Stoltz DA, 
            Devor EJ, Yan Z, Engelhardt JF
  TITLE     Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal 
            Cells Using miR-106b.
  JOURNAL   Genes (Basel). 2020 Oct 6;11(10). pii: genes11101169. doi: 
            10.3390/genes11101169.
  PUBMED    33036232
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2020-10-6"; volume: "11(10). pii: genes11101169. 
            doi"; pages: " 10.3390/genes11101169"
COMMENT     Sequence Label: pLV-PGK-dt/CBA-EGFP-R106bT
FEATURES             Location/Qualifiers
     source          1..9475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(710..733)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1107..1126
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1198..1899
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(1331..1349)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1599..1618
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1793..1816
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    1910..2498
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1963..1983)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2381..2392)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2571..2804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2876..2997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2913..2932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2967..2986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3037..3172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3192..3211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3193..3211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3221..3238)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3221..3237)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3230..3252)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3379..3834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3466..3485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3676..3697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3860..3964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3965..4033,4034..4825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4183..4202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4996..5584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5485..5504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5738..5755
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5872..5893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5908..5913,5914..5931,5932..5938)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5946..5962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5951..5973
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5970..5986
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5970..5986
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6005..6025
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6007..6025
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6053..6279
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6280..6460
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6507..6632
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7125..7358
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7543..7587
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7874..7991
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    8073..8297
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8286..8303
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8464..8484)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8488..8508)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8512..8532)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(8545..9255,9256..9258,9259..9261))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8545..9255,9256..9258,9259..9261))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8587..8608)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8936..8955
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9195..9216
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9258..9267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(9342..9475,1..141))
                     /label=chicken beta-actin promoter
ORIGIN
        1 gccccgcccc gcctggcgcg cgnccccccc cccccgcccc catcgctgca caaaataatt
       61 aaaaaataaa taaatacaaa attgggggtg gggagggggg ggagatgggg agagtgaagc
      121 agaacgtggg gctcacctcg accatggtaa tagcgatgac taatacgtag atgtactgcc
      181 aagtaggaaa gtcccgtaag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      241 cattgacgtc aatagggggc ggacttggca tatgatacac ttgatgtact gccaagtggg
      301 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      361 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      421 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
      481 ccgtaattga ttactattaa taactagtca ataatcaatg ccaacatggc ggtcatattg
      541 gacatgagcc aatataaatg tacatattat gatatagata caacgtatgc aatggccaat
      601 agccaatatt gatttatgct atataaccaa tgaataatat ggctaatggc caatattgaa
      661 gatcccccgg gctgcaggaa ttccagtact tttcccaagg cagtctggag catgcgcttt
      721 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
      781 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
      841 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
      901 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
      961 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     1021 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     1081 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     1141 gctgttctcc tcttcctcat ctccgggcct ttcgacctga agcttgccgc cggcaccatg
     1201 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     1261 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1321 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     1381 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     1441 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1501 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     1561 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     1621 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     1681 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     1741 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     1801 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     1861 caccacctgt tcctgtacgg catggacgag ctgtacaagt aactcgagta atcaacctct
     1921 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     1981 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2041 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2101 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2161 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2221 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2281 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     2341 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2401 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2461 tcagacgagt cggatctccc tttgggccgc ctccccgcag gtacctttaa gaccaatgac
     2521 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     2581 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     2641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     2821 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     2881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3001 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3061 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3121 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     3181 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     3241 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3301 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3361 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3421 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3481 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3541 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3601 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3661 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3721 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3781 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5761 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5821 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5881 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5941 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6001 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     6061 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     6121 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     6181 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     6241 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     6301 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6361 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6421 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     6481 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     6541 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     6601 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6661 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6721 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6781 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6841 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6901 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6961 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     7021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     7081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     7141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     7201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     7261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     7501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7801 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata caaatggcag
     7861 tattcattca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     7921 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     7981 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggtct agtggatcct
     8041 agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg
     8101 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
     8161 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
     8221 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
     8281 gcaactagaa ggcacagtcg aggctgatca gcttatcgac aacggcatcg ctactctaga
     8341 gagatctgca ctgtcagcac tttacgatat ctgcactgtc agcactttaa ccggtatctg
     8401 cactgtcagc actttatcac atctgcactg tcagcacttt attataagga tcctctagga
     8461 tcctaccttt ctcttctttt ttggatctac ctttctcttc ttttttggat ctacctttct
     8521 cttctttttt ggatcagctc gagtcttgta cagctcgtcc atgccgagag tgatcccggc
     8581 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     8641 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     8701 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     8761 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     8821 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     8881 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     8941 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     9001 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     9061 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     9121 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     9181 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     9241 ctcctcgccc ttgctcacca tggtggcggc cgctaccggg gcgggcggcg cgaggcggcg
     9301 gcggagcggg gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc
     9361 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
     9421 ctctgattgg ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gcccc
//
