LOCUS       sequence-324339-        9205 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses HPV33 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV33 L1L2
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..32)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        194..1500
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          521..1468
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3048..3169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3059..3078)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3113..3132
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3357..3945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3846..3865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4025..4328
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4443..5748
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4785..5716
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7186..7630
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7663..7709
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7701..7719
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7729..8523
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7729..8523
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7783..7802)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8393..8412
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8570..9142
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     enhancer        join(9194..9205,1..180)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
ORIGIN
        1 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
       61 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      181 actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc
      241 cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg
      301 aggagcatgc gctcagggct gagcgcgggg agagcagagc acacaagctc atagaccctg
      361 gtcgtggggg ggaggaccgg ggagctggcg cggggcaaac tgggaaagcg gtgtcgtgtg
      421 ctggctccgc cctcttcccg agggtggggg agaacggtat ataagtgcgg cagtcgcctt
      481 ggacgttctt tttcgcaacg ggtttgccgt cagaacgcag gtgaggggcg ggtgtggctt
      541 ccgcgggccg ccgagctgga ggtcctgctc cgagcgggcc gggccccgct gtcgtcggcg
      601 gggattagct gcgagcattc ccgcttcgag ttgcgggcgg cgcgggaggc agagtgcgag
      661 gcctagcggc aaccccgtag cctcgcctcg tgtccggctt gaggcctagc gtggtgtccg
      721 cgccgccgcc gcgtgctact ccggccgcac tctggtcttt tttttttttg ttgttgttgc
      781 cctgctgcct tcgattgccg ttcagcaata ggggctaaca aagggagggt gcggggcttg
      841 ctcgcccgga gcccggagag gtcatggttg gggaggaatg gagggacagg agtggcggct
      901 ggggcccgcc cgccttcgga gcacatgtcc gacgccacct ggatggggcg aggcctgggg
      961 tttttcccga agcaaccagg ctggggttag cgtgccgagg ccatgtggcc ccagcacccg
     1021 gcacgatctg gcttggcggc gccgcgttgc cctgcctccc taactagggt gaggccatcc
     1081 cgtccggcac cagttgcgtg cgtggaaaga tggccgctcc cgggccctgt tgcaaggagc
     1141 tcaaaatgga ggacgcggca gcccggtgga gcgggcgggt gagtcaccca cacaaaggaa
     1201 gagggcctgg tccctcaccg gctgctgctt cctgtgaccc cgtggtccta tcggccgcaa
     1261 tagtcacctc gggcttttga gcacggctag tcgcggcggg gggaggggat gtaatggcgt
     1321 tggagtttgt tcacatttgg tgggtggaga ctagtcaggc cagcctggcg ctggaagtca
     1381 tttttggaat ttgtcccctt gagttttgag cggagctaat tctcgggctt cttagcggtt
     1441 caaaggtatc ttttaaaccc ttttttaggt gttgtgaaaa ccaccgctaa ttcaaagcaa
     1501 ccggtgatat caaagatccg ccaccatgtc agtgtggaga ccttcagaag ctactgtgta
     1561 cttgccacct gtaccagtga gcaaggttgt gtcaacggac gagtatgttt cccggacgtc
     1621 catctattac tacgccggct cttccagact gctggcagtg ggacatccct attttagtat
     1681 caaaaaccct acaaatgcca aaaagcttct cgtcccaaaa gtgagcggcc tccagtatcg
     1741 agtgttcaga gtcagactcc cagaccccaa taagttcggt tttccggaca cctctttcta
     1801 caatcccgac acgcagcgac tggtatgggc atgtgttgga ctggagattg gcagaggcca
     1861 gcctcttggg gtgggcatca gcggacaccc gcttctgaac aagtttgatg acactgaaac
     1921 cggcaacaag taccctggtc agcctggcgc tgataatcgc gagtgtctgt ctatggacta
     1981 caagcagaca caactgtgtt tgctcggttg caaaccccct accggagagc actggggaaa
     2041 gggtgtcgct tgtacaaacg ccgcgccagc aaacgattgc ccaccccttg agctgatcaa
     2101 caccattatc gaagatgggg acatggtcga cactggcttc gggtgcatgg atttcaagac
     2161 actgcaggca aataaaagcg acgttcccat agatatttgc ggatcaacct gcaagtatcc
     2221 cgattatctt aaaatgacat ctgaaccgta cggcgacagt ctctttttct tcctgcggcg
     2281 cgagcaaatg ttcgtgcgcc attttttcaa tcgggccgga acactcgggg aggctgtgcc
     2341 tgatgatctg tacattaagg gctcaggcac caccgcctcc atccagtcca gtgcattctt
     2401 tcccactcca agcgggagta tggtgacatc tgaaagccag ttgtttaata aaccatattg
     2461 gctgcaaagg gcccagggcc acaataacgg gatatgctgg ggaaatcaag tatttgtgac
     2521 cgtcgtcgac actacacgga gtacaaacat gaccctgtgt acccaggtca ctagcgatag
     2581 tacctataag aacgaaaact ttaaggagta catccgccat gtggaggagt atgacttgca
     2641 gtttgttttc cagctgtgta aagtgacgtt gacagccgaa gtgatgactt acattcacgc
     2701 aatgaacccc gatatccttg aagattggca gtttggtctg acccctcccc caagcgcttc
     2761 cctccaagat acgtaccggt tcgttacatc acaagccatt acttgccaga agaccgtgcc
     2821 acccaaagag aaggaagacc ccttggggaa atacaccttc tgggaggtag acctcaagga
     2881 gaagttcagc gcggacctgg accagttccc cctgggcagg aagtttctgc tccaggccgg
     2941 gctgaaggcc aagcctaaat tgaaaagggc ggctccgacc tccactagga cttcttccgc
     3001 taaaaggaag aaagtcaaga aatgatagtc tagctggcca gacatgataa gatacattga
     3061 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3121 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3181 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3241 aaacctctac aaatgtggta tggaaatgtt aattaactag ccatgaccaa aatcccttaa
     3301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3421 gtggtttttt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt aattaacctg
     4021 caggcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     4081 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     4141 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     4201 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4261 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4321 ttaccatgat gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4381 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttga ctagtggagc
     4441 cgagagtaat tcatacaaaa ggagggatcg ccttcgcaag gggagagccc agggaccgtc
     4501 cctaaattct cacagaccca aatccctgta gccgccccac gacagcgcga ggagcatgcg
     4561 cccagggctg agcgcgggta gatcagagca cacaagctca cagtccccgg cggtgggggg
     4621 aggggcgcgc tgagcggggg ccagggagct ggcgcggggc aaactgggaa agtggtgtcg
     4681 tgtgctggct ccgccctctt cccgagggtg ggggagaacg gtatataagt gcggtagtcg
     4741 ccttggacgt tctttttcgc aacgggtttg ccgtcagaac gcaggtgagt ggcgggtgtg
     4801 gcttccgcgg gccccggagc tggagccctg ctctgagcgg gccgggctga tatgcgagtg
     4861 tcgtccgcag ggtttagctg tgagcattcc cacttcgagt ggcgggcggt gcgggggtga
     4921 gagtgcgagg cctagcggca accccgtagc ctcgcctcgt gtccggcttg aggcctagcg
     4981 tggtgtccgc cgccgcgtgc cactccggcc gcactatgcg ttttttgtcc ttgctgccct
     5041 cgattgcctt ccagcagcat gggctaacaa agggagggtg tggggctcac tcttaaggag
     5101 cccatgaagc ttacgttgga taggaatgga agggcaggag gggcgactgg ggcccgcccg
     5161 ccttcggagc acatgtccga cgccacctgg atggggcgag gcctgtggct ttccgaagca
     5221 atcgggcgtg agtttagcct acctgggcca tgtggcccta gcactgggca cggtctggcc
     5281 tggcggtgcc gcgttccctt gcctcccaac aagggtgagg ccgtcccgcc cggcaccagt
     5341 tgcttgcgcg gaaagatggc cgctcccggg gccctgttgc aaggagctca aaatggagga
     5401 cgcggcagcc cggtggagcg ggcgggtgag tcacccacac aaaggaagag ggccttgccc
     5461 ctcgccggcc gctgcttcct gtgaccccgt ggtctatcgg ccgcatagtc acctcgggct
     5521 tctcttgagc accgctcgtc gcggcggggg gaggggatct aatggcgttg gagtttgttc
     5581 acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt cttggaattt
     5641 gcccctttga gtttggagcg aggctaattc tcaagcctct tagcggttca aaggtatttt
     5701 ctaaacccgt ttccaggtgt tgtgaaagcc accgctaatt caaagcaatc cggagtatac
     5761 ggatccgcca ccatgcggca caagcggagc acccggcgga agagagccag cgccacccag
     5821 ctgtaccaga cctgcaaggc caccggcacc tgcccccctg acgtgatccc caaggtggag
     5881 ggcagcacaa tcgccgacca gatcctgaag tacggcagcc tgggcgtgtt cttcggcggc
     5941 ctgggcatcg gcacaggcag cggcagcggc ggcagaaccg gctacgtgcc catcggcacc
     6001 gaccctccca ccgccgccat ccccctgcag cccattagac cccctgtgac cgtggacacc
     6061 gtgggccccc tggacagcag catcgtgtcc ctgatcgagg aaaccagctt catcgaggca
     6121 ggggcaccag cccccagcat ccccaccccc agcggcttcg acgtgaccac ctccgccgac
     6181 accacccccg ccatcatcaa cgtgtccagc gtgggcgaga gcagcatcca gaccatcagc
     6241 acccacctga accccacctt caccgagccc agcgtgctgc accctcccgc ccctgccgag
     6301 gcctctggac acttcatctt cagcagcccc accgtgtcca cccagagcta cgagaacatc
     6361 cccatggaca ccttcgtggt gtccaccgac agctccaacg tgaccagcag cacccccatc
     6421 cccggcagca gacccgtggc ccggctgggc ctgtacagcc ggaacaccca gcaggtcaag
     6481 gtggtggacc ccgccttcct gaccagcccc cacaagctga tcacctacga caaccctgcc
     6541 ttcgagagct tcgaccccga ggacaccctg cagttccagc acagcgacat cagccctgcc
     6601 cccgaccccg atttcctgga catcattgcc ctgcacagac ccgccatcac cagcagacgg
     6661 cacaccgtgc ggttcagcag agtgggccag aaggccaccc tgaaaaccag aagcggcaag
     6721 cagatcggcg ccagaatcca ctactaccag gacctgagcc ccatcgtgcc cctggaccac
     6781 accgtgccca acgagcagta cgagctgcag cccctgcacg acaccagcac cagcagctac
     6841 agcatcaacg acggcctgta cgacgtgtac gccgacgacg tggacaacgt gcacaccccc
     6901 atgcagcaca gctactccac cttcgccacc acccggacca gcaatgtgtc catccctctg
     6961 aacaccggct tcgacacccc cgtgatgagc ggccctgaca tccccagccc cctgttcccc
     7021 acctcctccc ccttcgtccc tatcagccct ttcttccctt tcgataccat cgtggtggac
     7081 ggcgccgact ttgtgctgca ccccagctac ttcatcctgc ggcggaggcg gaagcggttc
     7141 ccctacttct tcaccgacgt gcgggtggcc gcctgatagt ctaggagcag gtttccccaa
     7201 tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc caggtctaga ggggtaacac
     7261 tttgtactgc gtttggctcc acgctcgatc cactggcgag tgttagtaac agcactgttg
     7321 cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg gtaacaagga cccacggggc
     7381 caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc cagcacggcg actttactgc
     7441 gaaacccact ttaaagtgac attgaaactg gtacccacac actggtgaca ggctaaggat
     7501 gcccttcagg taccccgagg taacacgcga cactcgggat ctgagaaggg gactggggct
     7561 tctataaaag cgctcggttt aaaaagcttc tatgcctgaa taggtgaccg gaggtcggca
     7621 cctttccttt gcaattactg accctatgaa tacactgact gtttgacaat taatcatcgg
     7681 catagtatat cggcatagta taatacgact cactatagga gggccaccat gattgaacaa
     7741 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7801 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7861 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     7921 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7981 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     8041 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     8101 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     8161 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     8221 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     8281 gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8341 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8401 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8461 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8521 tgagcgggac tctggggttc gaaatgaccg accaagcgaa ttcgctagga ttatccctaa
     8581 tacctgccac cccactctta atcagtggtg gaagaacggt ctcagaactg tttgtttcaa
     8641 ttggccattt aagtttagta gtaaaagact ggttaatgat aacaatgcat cgtaaaacct
     8701 tcagaaggaa aggagaatgt tttgtggacc actttggttt tcttttttgc gtgtggcagt
     8761 tttaagttat tagtttttaa aatcagtact ttttaatgga aacaacttga ccaaaaattt
     8821 gtcacagaat tttgagaccc attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt
     8881 caacaccgag acatttaggt gaaagacatc taattctggt tttacgaatc tggaaacttc
     8941 ttgaaaatgt aattcttgag ttaacacttc tgggtggaga atagggttgt tttcccccca
     9001 cataattgga aggggaagga atatcattta aagctatggg agggttgctt tgattacaac
     9061 actggagaga aatgcagcat gttgctgatt gcctgtcact aaaacaggcc aaaaactgag
     9121 tccttgggtt gcatagaaag ctgcctgcag ggcctgaaat aacctctgaa agaggaactt
     9181 ggttaggtac cttctgaggc ggaaa
//
