LOCUS       sequence-324499-        9349 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Contains bidirectional reporter genes dtomato and nuclear-targeting 
            EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Choi SH, Reeves RE, Romano Ibarra GS, Lynch TJ, Shahin WS, Feng Z, 
            Gasser GN, Winter MC, Evans TIA, Liu X, Luo M, Zhang Y, Stoltz DA, 
            Devor EJ, Yan Z, Engelhardt JF
  TITLE     Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal 
            Cells Using miR-106b.
  JOURNAL   Genes (Basel). 2020 Oct 6;11(10). pii: genes11101169. doi: 
            10.3390/genes11101169.
  PUBMED    33036232
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2020-10-6"; volume: "11(10). pii: genes11101169. 
            doi"; pages: " 10.3390/genes11101169"
COMMENT     Sequence Label: pLV-PGK-dt/CBA-EGFP-NLS
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        69..344
                     /label=chicken beta-actin promoter
     regulatory      419..428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(425..427,428..430,431..1141)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(425..427,428..430,431..1141)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(470..491)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(731..750)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1078..1099
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1154..1174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1178..1198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1202..1222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1256..1273)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1262..1486
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1568..1685
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1972..2016)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2201..2434
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2927..3052
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3099..3279
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3280..3506)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3534..3554)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3534..3552)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3573..3589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3573..3589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3586..3608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3597..3613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3621..3627,3628..3645,3646..3651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3666..3687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3804..3821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3975..4563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4055..4074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4734..5525,5526..5594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5357..5376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5595..5699)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5725..6180)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5862..5883)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6074..6093
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6307..6329
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6321..6338
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6322..6338
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6348..6367
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6348..6366
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6387..6522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6562..6683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6573..6592)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6627..6646
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6755..6988
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7061..7649
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7167..7178
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7576..7596
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7660..8361)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(7743..7766)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7941..7960)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8210..8228
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8433..8452)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8826..8849
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        join(9037..9349,1..67)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
       61 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
      121 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
      181 ggggggngcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      241 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      301 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      361 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg tagcggccgc
      421 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      481 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      541 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      601 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      661 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      721 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      781 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      841 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      901 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      961 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1021 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1081 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1141 gactcgagct gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga
     1201 tccaaaaaag aagagaaagg taggatccta gaggatcctt aattaagctg atcagcctcg
     1261 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     1321 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     1381 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     1441 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     1501 agaaccagct ggggctctag gatccactag accaaagtgg atctctgctg tccctgtaat
     1561 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
     1621 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
     1681 taaaattgtg aatgaatact gccatttgta tcgataccgt cgagatccgt tcactaatcg
     1741 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
     1801 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     1861 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     1921 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     1981 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     2041 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     2101 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     2161 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     2221 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     2281 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     2341 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     2401 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     2461 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     2521 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     2581 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     2641 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     2701 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     2761 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     2821 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     2881 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     2941 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     3001 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     3061 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     3121 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     3181 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     3241 gagctcccag gctcagatct ggtctaacca gagagacccg tttatgtatc gagctaggca
     3301 cttaaataca atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc
     3361 cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc
     3421 acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga
     3481 ctacaagagt attgcataag actacattaa gcttgcagct ccagcttttg ttccctttag
     3541 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3721 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4381 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4621 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5701 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     5761 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5821 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5881 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5941 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6001 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6061 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6121 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6181 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6241 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6301 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     6361 tatagggcga attgggtacg tccctaggcc tccaaaaaag cctcctcact acttctggaa
     6421 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     6481 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     6541 gatagctaga gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     6601 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6661 ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat
     6721 aaatactgaa taataagatg acatgaacta ctactgctag agattttcca cactgactaa
     6781 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     6841 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     6901 tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt
     6961 cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga
     7021 tctacagctg ccttgtaagt cattggtctt aaaggtacct gcggggaggc ggcccaaagg
     7081 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     7141 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     7201 ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt cagcttcccc
     7261 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     7321 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     7381 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     7441 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     7501 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     7561 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     7621 tcacaaattt tgtaatccag aggttgatta ctcgagttac ttgtacagct cgtccatgcc
     7681 gtacaggaac aggtggtggc ggccctcgga gcgctcgtac tgttccacga tggtgtagtc
     7741 ctcgttgtgg gaggtgatgt ccagcttggt gtccacgtag tagtagccgg gcagttgcac
     7801 gggcttcttg gccatgtaga tggtcttgaa ctccaccagg tagtggccgc cgtccttcag
     7861 cttcagggcc tggtggatct cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt
     7921 ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggggg ggaagttggt
     7981 gccgcgcatc ttcaccttgt agatcagcgt gccgtcctgc agggaggagt cctgggtcac
     8041 ggtcaccaga ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa
     8101 ggacagcttc ttgtaatcgg ggatgtcggc ggggtgcttc acgtacgcct tggagccgta
     8161 catgaactgg ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt
     8221 cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa
     8281 ctcgtggccg ttcatggagc cctccatgcg caccttgaag cgcatgaact ctttgatgac
     8341 ctcctcgccc ttgctcacca tggtgccggc ggcaagcttc aggtcgaaag gcccggagat
     8401 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
     8461 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
     8521 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
     8581 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
     8641 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
     8701 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
     8761 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
     8821 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag tactggaatt
     8881 cctgcagccc gggggatctt caatattggc cattagccat attattcatt ggttatatag
     8941 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     9001 tttatattgg ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt
     9061 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     9121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     9181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     9241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
     9301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgccca
//
