LOCUS       sequence-324898-        9126 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_APOBEC1-UdgX-NG-nCas9
FEATURES             Location/Qualifiers
     source          1..9126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             715..1398
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2218..6318
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6361..6381
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6390..6407
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6430..6447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6436..6660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6731..6747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6731..6747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6744..6766)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6755..6771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6779..6785,6786..6803,6804..6809))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6824..6845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6962..6979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7133..7721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7213..7232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7892..8683,8684..8752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8515..8534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8753..8857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8936..8955)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtctcctca
      721 gagactgggc ctgtcgccgt cgatccaacc ctgcgccgcc ggattgaacc tcacgagttt
      781 gaagtgttct ttgacccccg ggagctgaga aaggagacat gcctgctgta cgagatcaac
      841 tggggaggca ggcactccat ctggaggcac acctctcaga acacaaataa gcacgtggag
      901 gtgaacttca tcgagaagtt taccacagag cggtacttct gccccaatac cagatgtagc
      961 atcacatggt ttctgagctg gtccccttgc ggagagtgta gcagggccat caccgagttc
     1021 ctgtccagat atccacacgt gacactgttt atctacatcg ccaggctgta tcaccacgca
     1081 gacccaagga ataggcaggg cctgcgcgat ctgatcagct ccggcgtgac catccagatc
     1141 atgacagagc aggagtccgg ctactgctgg cggaacttcg tgaattattc tcctagcaac
     1201 gaggcccact ggcctaggta cccacacctg tgggtgcgcc tgtacgtgct ggagctgtat
     1261 tgcatcatcc tgggcctgcc cccttgtctg aatatcctgc ggagaaagca gccccagctg
     1321 accttcttta caatcgccct gcagtcttgt cactatcaga ggctgccacc ccacatcctg
     1381 tgggccacag gcctgaagtc tggaggatct agcggaggat cctctggcag cgagacacca
     1441 ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg cagcatggcc
     1501 ggagcacagg attttgtccc acatactgcc gatctggctg agctggctgc cgccgctgga
     1561 gagtgtagag gatgcgggct gtaccgggat gccacacagg ccgtgttcgg agcaggcggc
     1621 aggagcgccc gcatcatgat gatcggcgag cagccaggcg ataaggagga cctggccggc
     1681 ctgccatttg tgggaccagc aggccggctg ctggacagag ccctggaggc cgccgacatc
     1741 gatagggacg ccctgtacgt gaccaacgcc gtgaagcact tcaagtttac acgcgccgca
     1801 ggaggcaaga ggagaatcca caagaccccc tctcggacag aggtggtggc ctgcagacct
     1861 tggctgatcg ccgagatgac cagcgtggag ccagatgtgg tggtgctgct gggagcaaca
     1921 gcagcaaagg ccctgctggg caatgacttc agggtgaccc agcacagggg agaggtgctg
     1981 cacgtggacg atgtgccagg cgatcctgcc ctggtggcaa ccgtgcaccc tagctccctg
     2041 ctgagaggcc caaaggagga gcgggaatcc gccttcgctg ggctggtgga cgacctgaga
     2101 gtggctgccg atgtgagacc tagcggtggt agttctggcg gtagctcagg atctgaaact
     2161 cccggcacat ctgaaagcgc gactcccgaa tcatctggtg gatcttcagg tggaagtgac
     2221 aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc
     2281 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
     2341 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
     2401 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
     2461 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
     2521 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
     2581 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
     2641 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
     2701 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
     2761 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
     2821 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
     2881 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
     2941 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
     3001 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
     3061 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
     3121 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
     3181 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
     3241 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
     3301 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
     3361 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
     3421 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
     3481 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
     3541 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
     3601 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
     3661 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
     3721 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
     3781 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
     3841 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
     3901 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
     3961 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
     4021 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
     4081 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
     4141 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
     4201 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
     4261 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
     4321 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
     4381 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
     4441 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
     4501 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
     4561 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
     4621 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
     4681 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg
     4741 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
     4801 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
     4861 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
     4921 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
     4981 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
     5041 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
     5101 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
     5161 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
     5221 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
     5281 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
     5341 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
     5401 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
     5461 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
     5521 ggcggcttca gcaaagagtc tatcaggccc aagaggaaca gcgataagct gatcgccaga
     5581 aagaaggact gggaccctaa gaagtacggc ggcttcgtca gccccaccgt ggcctattct
     5641 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
     5701 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
     5761 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
     5821 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccagatt cctgcagaag
     5881 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
     5941 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
     6001 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
     6061 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
     6121 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctagg
     6181 gccttcaagt actttgacac caccatcgac cggaaggtgt acaggagcac caaagaggtg
     6241 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
     6301 tctcagctgg gaggtgactc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     6361 cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc
     6421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6661 cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg
     6721 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6781 acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca
     6841 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6901 attaatgaat cggccaacgc gcgggaagag gcggtttgcg tattgggcgc tcttccgctt
     6961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7561 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7741 ctacggggtc tgacactcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8881 ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa
     8941 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     9001 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     9061 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     9121 cgcgtt
//
