LOCUS       sequence-32491-e         867 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-1188
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(468..492)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             518..559
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(599..618)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    643..691
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gctactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctg gctagttaag ctatcaacaa gtttgtacaa aaaagcaggc tcaccctagt
      121 tgaagccctg agctgggtgg gctgtctgtc cagaactcac atgggaaatt tcacaaagag
      181 gaaaagtctc atgatctcca ttggattggt gaagacacca tggcacagaa aagcaaagaa
      241 gtccaatact cacagtctcc cagctggtgt gaggttgggc caggatgaaa cccaaggctc
      301 tccgaggctc cccaccacac cctgctgctg aagactgcct agcaaggctg tgccgagtgg
      361 tgtgggtggg caaaggatgg attgactagt tataagcacc tcccagaagt ccttgagatc
      421 tcagctattt tgtggcccaa tccctttgaa gggcagggtc aaaattaacc cagctttctt
      481 gtacaaagtg gttgatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      541 cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacggggg aggctaactg
      601 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
      661 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
      721 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
      781 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
      841 gcaggccctg ccatagcaga tctgcgc
//
