LOCUS       sequence-325311-        8816 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Tet-inducible shRNA targeting human METTL3 #1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Cotter KA, Gallon J, Uebersax N, Rubin P, Meyer KD, Piscuoglio S, 
            Jaffrey SR, Rubin MA
  TITLE     Mapping of m6A and Its Regulatory Targets in Prostate Cancer Reveals
            a METTL3-low Induction of Therapy Resistance.
  JOURNAL   Mol Cancer Res. 2021 Jun 4. pii: 1541-7786.MCR-21-0014. doi: 
            10.1158/1541-7786.MCR-21-0014.
  PUBMED    34088870
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2021 Jun 4. pii: 1541-7786.MCR-21-0014. doi: 
            10.1158/1541-7786.MCR-21-0014."
COMMENT     Sequence Label: METTL3 shRNA 1 tet pLKO puro
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1597..1691
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     1608..1627
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    1645..1663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1673..1691
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1792..1909
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1958..2468
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2387..2405
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2477..3049
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3057..3076
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3104..3122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3133..3753
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3206..3225)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    3788..4361
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3955..3972)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4182..4201
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4381..4980
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4381..4400)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4877..4897
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5108..5341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5413..5534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5450..5469)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5504..5523
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5574..5709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5730..5748)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5758..5775)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5758..5774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5767..5789)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5916..6371
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6003..6022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6213..6234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6397..6501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6502..6570,6571..7362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6720..6739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7533..8121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8022..8041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8275..8292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8409..8430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8445..8450,8451..8468,8469..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8483..8499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8488..8510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8507..8523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8507..8523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8542..8562
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8544..8562
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8590..8816
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat cacgagacta gcctcgagcg gccgcaatat ttgcatgtcg ctatgtgttc
     1621 tgggaaatca ccataaacgt gaaatcccta tcagtgatag agacttataa gttccctatc
     1681 agtgatagag acaccgggca agtatgttca ctatgaaact cgagtttcat agtgaacata
     1741 cttgcttttt gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
     1801 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     1861 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     1921 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc
     1981 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
     2041 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     2101 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     2161 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     2221 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     2281 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     2341 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     2401 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     2461 ctccccaggg ggatctgtga gtttggggac ccttgattgt tctttctttt tcgctattgt
     2521 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
     2581 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg
     2641 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag
     2701 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt
     2761 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt
     2821 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg
     2881 ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca tcctgccttt
     2941 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa
     3001 accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     3061 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgtaatacg actcactata
     3121 gggcgaattg atatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg
     3181 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
     3241 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
     3301 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
     3361 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
     3421 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
     3481 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
     3541 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
     3601 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
     3661 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
     3721 ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gcggctcccg ggagttctag
     3781 ggatctgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     3841 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     3901 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3961 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     4021 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     4081 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     4141 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     4201 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     4261 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     4321 accacgggga cgtggttttc ctttgaaaaa cacgatgata aggatccacc ggagcttacc
     4381 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4441 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4501 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4561 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4621 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4681 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4741 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4801 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4861 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4921 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     4981 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcggta
     5041 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5101 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     5161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5341 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     5401 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5521 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     5581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5641 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5701 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5761 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5821 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5881 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     5941 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6001 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6061 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6121 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6181 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6241 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6301 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     6361 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6421 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6721 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6781 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6841 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6901 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6961 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7021 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7081 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7141 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7201 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7261 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7321 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7381 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7441 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7501 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7561 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7621 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7681 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7741 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7801 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7861 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7921 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7981 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8041 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8101 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8161 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8221 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8281 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8341 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8521 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     8581 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     8641 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     8701 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     8761 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaac
//
