LOCUS       sequence-325470-       11121 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Destination vector expressing plant-codon-optimized Cas9 under 35S 
            promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat 
            specific red fluorescence for screening trangene free.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, 
            Schwab R, Weigel D
  TITLE     An efficient CRISPR vector toolbox for engineering large deletions 
            in Arabidopsis thaliana.
  JOURNAL   Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. 
            eCollection 2018.
  PUBMED    30083222
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-08-2"; volume: "14:65. doi"; pages: " 
            10.1186/s13007-018-0330-7. eCollection 2018"
COMMENT     Sequence Label: pRWJB006
FEATURES             Location/Qualifiers
     source          1..11121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..23
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        559..904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     847..867
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             933..980
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             987..1007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1032..2468,2658..5321)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2469..2657
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5322..5369
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5375..6016
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(6239..6562)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     6472..6488
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6558..6574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6558..6574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6571..6593)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6582..6598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6606..6612,6613..6630,6631..6636))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6651..6672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6789..6806)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7185..7437)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(7721..7740)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7990..8008
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8607..8631
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8722..9310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8802..8821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9481..10269)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10560..10995
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tggcaggata tattgtggtg taacgttctc gaactaggct cggacgaagt aagcggccgc
       61 attcggtccc cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag
      121 agaggcttac gcagcacgtc tcatcaagac gatctacccg agcaataatc tccaggaaat
      181 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa
      241 gaacacagag aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca
      301 aggcttgctt cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc
      361 cactgaatca aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt
      421 aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt
      481 cgtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc
      541 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg
      601 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
      661 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
      721 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      781 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      841 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
      901 aacacggggg acgagctaac agcggatcca tggattacaa ggatgatgat gataaggatt
      961 acaaggatga tgatgataag atggctccaa agaagaagag aaaggttgga atccacggag
     1021 ttccagctgc tgataagaag tactctatcg gacttgacat cggaaccaac tctgttggat
     1081 gggctgttat caccgatgag tacaaggttc catctaagaa gttcaaggtt cttggaaaca
     1141 ccgatagaca ctctatcaag aagaacctta tcggtgctct tcttttcgat tctggagaaa
     1201 ccgctgaggc taccagattg aagagaaccg ctagaagaag atacaccaga agaaagaaca
     1261 gaatctgcta ccttcaggaa atcttctcta acgagatggc taaggttgat gattctttct
     1321 tccacagact tgaggagtct ttccttgttg aggaggataa gaagcacgag agacacccaa
     1381 tcttcggaaa catcgttgat gaggttgctt accacgagaa gtacccaacc atctaccacc
     1441 ttagaaagaa gttggttgat tctaccgata aggctgatct tagacttatc taccttgctc
     1501 ttgctcacat gatcaagttc agaggacact tccttatcga gggtgacctt aacccagata
     1561 actctgatgt tgataagttg ttcatccagc ttgttcagac ctacaaccag cttttcgagg
     1621 agaacccaat caacgcttct ggagttgatg ctaaggctat cctttctgct agactttcta
     1681 agtctcgtag acttgagaac cttatcgctc agcttccagg agagaagaag aacggacttt
     1741 tcggaaacct tatcgctctt tctcttggac ttaccccaaa cttcaagtct aacttcgatc
     1801 ttgctgagga tgctaagttg cagctttcta aggataccta cgatgatgat cttgataacc
     1861 ttcttgctca gatcggagat cagtacgctg atcttttcct tgctgctaag aacctttctg
     1921 atgctatcct tctttctgac atccttagag ttaacaccga gatcaccaag gctccacttt
     1981 ctgcttctat gatcaagaga tacgatgagc accaccagga tcttaccctt ttgaaggctc
     2041 ttgttagaca gcagcttcca gagaagtaca aggaaatctt cttcgatcag tctaagaacg
     2101 gatacgctgg atacatcgat ggaggagctt ctcaggagga gttctacaag ttcatcaagc
     2161 caatccttga gaagatggat ggaaccgagg agcttcttgt taagttgaac agagaggatc
     2221 ttcttagaaa gcagagaacc ttcgataacg gatctatccc acaccagatc caccttggag
     2281 agcttcacgc tatccttcgt agacaggagg atttctaccc attcttgaag gataacagag
     2341 agaagatcga gaagatcctt accttcagaa tcccatacta cgttggacca cttgctagag
     2401 gaaactctcg tttcgcttgg atgaccagaa agtctgagga aaccatcacc ccttggaact
     2461 tcgaggaggt aagtttctgc ttctaccttt gatatatata taataattat cattaattag
     2521 tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg
     2581 cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa
     2641 aatttgttga tgtgcaggtt gttgataagg gagcttctgc tcagtctttc atcgagagaa
     2701 tgaccaactt cgataagaac cttccaaacg agaaggttct tccaaagcac tctcttcttt
     2761 acgagtactt caccgtttac aacgagctta ccaaggttaa gtacgttacc gagggaatga
     2821 gaaagccagc tttcctttct ggagagcaga agaaggctat cgttgatctt cttttcaaga
     2881 ccaacagaaa ggttaccgtt aagcagttga aggaggatta cttcaagaag atcgagtgct
     2941 tcgattctgt tgaaatctct ggagttgagg atagattcaa cgcttctctt ggaacctacc
     3001 acgatctttt gaagatcatc aaggataagg atttccttga taacgaggag aacgaggaca
     3061 tccttgagga catcgttctt acccttaccc ttttcgagga tagagagatg atcgaggaga
     3121 gactcaagac ctacgctcac cttttcgatg ataaggttat gaagcagttg aagagaagaa
     3181 gatacaccgg atggggtaga ctttctcgta agttgatcaa cggaatcaga gataagcagt
     3241 ctggaaagac catccttgat ttcttgaagt ctgatggatt cgctaacaga aacttcatgc
     3301 agcttatcca cgatgattct cttaccttca aggaggacat ccagaaggct caggtttctg
     3361 gacagggaga ttctcttcac gagcacatcg ctaaccttgc tggatctcca gctatcaaga
     3421 agggaatcct tcagaccgtt aaggttgttg atgagcttgt taaggttatg ggtagacaca
     3481 agccagagaa catcgttatc gagatggcta gagagaacca gaccacccag aagggacaga
     3541 agaactctcg tgagagaatg aagagaatcg aggagggaat caaggagctt ggatctcaaa
     3601 tcttgaagga gcacccagtt gagaacaccc agcttcagaa cgagaagttg tacctttact
     3661 accttcagaa cggaagagat atgtacgttg atcaggagct tgacatcaac agactttctg
     3721 attacgatgt tgatcacatc gttccacagt ctttcttgaa ggatgattct atcgataaca
     3781 aggttcttac ccgttctgat aagaacagag gaaagtctga taacgttcca tctgaggagg
     3841 ttgttaagaa gatgaagaac tactggagac agcttcttaa cgctaagttg atcacccaga
     3901 gaaagttcga taaccttacc aaggctgaga gaggaggact ttctgagctt gataaggctg
     3961 gattcatcaa gagacagctt gttgaaacca gacagatcac caagcacgtt gctcagatcc
     4021 ttgattctcg tatgaacacc aagtacgatg agaacgataa gttgatcaga gaggttaagg
     4081 ttatcacctt gaagtctaag ttggtttctg atttcagaaa ggatttccag ttctacaagg
     4141 ttagagagat caacaactac caccacgctc acgatgctta ccttaacgct gttgttggaa
     4201 ccgctcttat caagaagtac ccaaagttgg agtctgagtt cgtttacgga gattacaagg
     4261 tttacgatgt tagaaagatg atcgctaagt ctgagcagga gatcggaaag gctaccgcta
     4321 agtacttctt ctactctaac atcatgaact tcttcaagac cgagatcacc cttgctaacg
     4381 gagagatcag aaagaggcca cttatcgaaa ccaacggaga aaccggagag atcgtttggg
     4441 ataagggaag agatttcgct accgttagaa aggttctttc tatgccacag gttaacatcg
     4501 ttaagaaaac cgaggttcag accggaggat tctctaagga gtctatcctt ccaaagagaa
     4561 actctgataa gttgatcgct agaaagaagg attgggaccc aaagaagtac ggaggattcg
     4621 attctccaac cgttgcttac tctgttcttg ttgttgctaa ggttgagaag ggaaagtcta
     4681 agaagttgaa gtctgttaag gagcttcttg gaatcaccat catggagcgt tcttctttcg
     4741 agaagaaccc aatcgatttc cttgaggcta agggatacaa ggaggttaag aaggatctta
     4801 tcatcaagtt gccaaagtac tctcttttcg agcttgagaa cggaagaaag agaatgcttg
     4861 cttctgctgg agagcttcag aagggaaacg agcttgctct tccatctaag tacgttaact
     4921 tcctttacct tgcttctcac tacgagaagt tgaagggatc tccagaggat aacgagcaga
     4981 agcagctttt cgttgagcag cacaagcact accttgatga gatcatcgag caaatctctg
     5041 agttctctaa gagagttatc cttgctgatg ctaaccttga taaggttctt tctgcttaca
     5101 acaagcacag agataagcca atcagagagc aggctgagaa catcatccac cttttcaccc
     5161 ttaccaacct tggtgctcca gctgctttca agtacttcga taccaccatc gatagaaaaa
     5221 gatacacctc taccaaggag gttcttgatg ctacccttat ccaccagtct atcaccggac
     5281 tttacgagac cagaatcgat ctttctcagc ttggaggaga taagagacca gctgctacca
     5341 agaaggctgg acaggctaag aagaagaagt gaaaagcttt cgttcgtatc atcggtttcg
     5401 acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa tcctactgag
     5461 tttgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt
     5521 tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga
     5581 gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct
     5641 tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa
     5701 aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacacttgta
     5761 gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta gaaaagctgc
     5821 aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa ctttccttta
     5881 tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat agttatactc
     5941 atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt gccaattgat
     6001 tgacaacatg catcaatcgg taccacctag tagaagagct cagtgagacc gaattcgctt
     6061 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     6121 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctaagcttcg gatgccggga
     6181 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     6241 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     6301 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     6361 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     6421 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     6481 cggccagtga attcgagctc ggtacacagg gatcttcaag agtcgacctg caggcatgca
     6541 ggcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6601 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6661 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6721 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6841 ctagtaggag gtaatcaatg ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6901 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6961 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     7021 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     7081 tcagtacaat ctgctctgat gccgcatgat catctggcgg acctcttcgg aggtccgctt
     7141 ttttttctcg agggtctcaa ctagctcttc atgggtattc tagagataat ttatttgaaa
     7201 attcataaga aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc
     7261 acgcacattt aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg
     7321 acaggagcat gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat
     7381 gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaagagc
     7441 tctcacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
     7501 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
     7561 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
     7621 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
     7681 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
     7741 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc
     7801 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
     7861 cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc
     7921 acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg
     7981 gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
     8041 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
     8101 cgcatgaact ccttgatgat ggccatgtta tccattgttc gtaccgtttt gctatttgtg
     8161 tatgttttct tgttttggtt tgagtgaaga gtgtagtgaa gggttggttt gtatttatag
     8221 gtgagaaagg agatttgcat ggcaatcacg tgtaagaatg catgcatgca tgagtgtgtg
     8281 tggagagaga gagatgctct tacgttgttg tcgggattat gtgttggaaa agagtgacaa
     8341 gtgacgaaca aaacaatacc actcatcata gctccgctta tgcatgctat gtgtgatttg
     8401 tagctcttgt tctgacatga cactatgaac aattttttaa aaagaaggtt aaacttttat
     8461 ttaaataaaa accctatgtt aatttggttt caatcgagta agatgagaag tcaatctttt
     8521 gatggtagct agtgatgttg gaaatatagt tgtgccagct tatctagacc aagcatgcac
     8581 agctggcgtt ctcagccaga tccccttgac aggatatatt ggcgggtaaa ctaagtcgct
     8641 gtatgtgttt gtttgagatc tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8881 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9121 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9181 cgctctgctg aagccagtta ccttcggaag aagagttggt agctcttgat ccggcaaaca
     9241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9421 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gtgtaacatt ggtctagtga
     9481 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tgaacaaatt cttccaactg
     9541 atctgcgcgc gaggccaagc gatcttcttg tccaagataa gcctgcctag cttcaagtat
     9601 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     9661 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     9721 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     9781 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     9841 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     9901 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     9961 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
    10021 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
    10081 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
    10141 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
    10201 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
    10261 ttccctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
    10321 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
    10381 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
    10441 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
    10501 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcgatccc
    10561 catccaacag cccgccgtcg agcgggcttt tttatccccg gaagcctgtg gatagagggt
    10621 agttatccac gtgaaaccgc taatgccccg caaagccttg attcacgggg ctttccggcc
    10681 cgctccaaaa actatccacg tgaaatcgct aatcagggta cgtgaaatcg ctaatcggag
    10741 tacgtgaaat cgctaataag gtcacgtgaa atcgctaatc aaaaaggcac gtgagaacgc
    10801 taatagccct ttcagatcaa cagcttgcaa acacccctcg ctccggcaag tagttacagc
    10861 aagtagtatg ttcaattagc ttttcaatta tgaatatata tatcaattat tggtcgccct
    10921 tggcttgtgg acaatgcgct acgcgcaccg gctccgcccg tggacaaccg caagcggttg
    10981 cccaccgtcg agcgcctttg cccacaaccc ggcggccggc cgcaacagat cgttttataa
    11041 attttttttt ttgaaaaaga aaaagcccga aaggcggcaa cctctcgggc ttctggattt
    11101 ccgatccccg gaattagatc t
//
