LOCUS       sequence-325475-        6127 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses mouse Six1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Menendez L, Trecek T, Gopalakrishnan S, Tao L, Markowitz AL, Yu HV, 
            Wang X, Llamas J, Huang C, Lee J, Kalluri R, Ichida J, Segil N
  TITLE     Generation of inner ear hair cells by direct lineage conversion of 
            primary somatic cells.
  JOURNAL   Elife. 2020 Jun 30;9. pii: 55249. doi: 10.7554/eLife.55249.
  PUBMED    32602462
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-30"; volume: "9. pii: 55249. doi"; pages: " 
            10.7554/eLife.55249"
COMMENT     Sequence Label: pMXs-Six1
FEATURES             Location/Qualifiers
     source          1..6127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..358
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             417..833
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     763..785
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     801..817
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    843..1217
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1155..1171
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1260..1284
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2146..2170)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2187..2775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2240..2260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2658..2669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2955..3424
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3554..3571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3725..4313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3805..3824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4477..5268,5269..5337))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5100..5119
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5338..5442)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5472..6065
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat
       61 gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
      121 tgacgagttc ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg
      181 tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc
      241 cctaatagga gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
      301 cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca
      361 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
      421 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      481 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
      541 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
      601 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
      661 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
      721 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
      781 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
      841 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
      901 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
      961 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1021 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1081 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1141 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1201 gaccatcctc tagactgccg gatctagcta gttaatcgac ggtatcgata agctatcaaa
     1261 caagtttgta caaaaaagca ggctgccacc atgtcgatgc tgccgtcgtt tggttttacg
     1321 caagagcaag tggcgtgcgt gtgcgaagtt ctgcagcaag gagggaacct ggaacgcctg
     1381 ggcaggttct tgtggtcgtt gcccgcctgc gatcacctgc acaagaacga gagcgtgctc
     1441 aaggccaagg cggtggtcgc cttccaccgc ggcaacttcc gcgagctcta caagatactg
     1501 gagagccacc agttctcgcc tcacaatcac cccaaactgc agcagctgtg gctgaaagcg
     1561 cactacgtgg aggccgagaa acttcgcggc cgacccctgg gtgccgtggg caaatatcgg
     1621 gtgcgccgaa aattcccgtt gccgcggacc atctgggacg gcgaggagac cagctactgc
     1681 tttaaggaga agtctcgggg cgtgctgcgg gagtggtacg cgcacaaccc ctacccctca
     1741 ccgagggaga aacgggagct ggccgaggcc accggcctca ccaccaccca ggtcagcaac
     1801 tggtttaaga accggaggca aagagaccgg gccgccgagg ccaaggaaag ggagaacacc
     1861 gaaaacaata actcctcctc caacaagcag aatcaactct ctcctctgga agggggcaag
     1921 ccgctcatgt ccagctcaga agaggagttc tcaccccccc aaagtccaga ccagaactcg
     1981 gtccttctgc tccagagcaa tatgggccac gccaggagct caaactattc tcttccaggc
     2041 ctcacagcct cccagcccag ccacggtctg caagcccatc agcaccagct ccaggactct
     2101 ctgctgggcc cactcacctc cagtttggtg gacttgggtt cctaaaccca gctttcttgt
     2161 acaaagtggt tcgatagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2221 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2281 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2341 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2401 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2461 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2521 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2581 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2641 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2701 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2761 gggccgcctc cccgcatcga taccgtcgac gataaaataa aagattttat ttagtctcca
     2821 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     2881 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     2941 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     3001 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     3061 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     3121 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     3181 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     3241 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     3301 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     3361 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     3421 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     3481 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3541 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     4021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     4081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4141 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc
     4441 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4501 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4561 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4621 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4681 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4741 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4801 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4861 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4921 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4981 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5041 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5101 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5161 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5221 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5281 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5341 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5401 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5461 gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     5521 ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc aaggtcagga
     5581 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     5641 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     5701 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     5761 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     5821 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     5881 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgattgac
     5941 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     6001 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     6061 tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc
     6121 gggaggt
//
