LOCUS       sequence-325507-        6472 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses TPMV H glycoprotein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Argaw T, Marino MP, Timmons A, Eldridge L, Takeda K, Li P, Kwilas A,
            Ou W, Reiser J
  TITLE     In vivo targeting of lentiviral vectors pseudotyped with the Tupaia 
            paramyxovirus H glycoprotein bearing a cell-specific ligand.
  JOURNAL   Mol Ther Methods Clin Dev. 2021 Apr 24;21:670-680. doi: 
            10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11.
  PUBMED    34141822
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2021-04-24"; volume: "21:670-680. doi"; 
            pages: " 10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11"
COMMENT     Sequence Label: pCG-TPMV Hd32
FEATURES             Location/Qualifiers
     source          1..6472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          325..898
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     906..925
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        953..971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      991..1000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2923..2940
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3133..3152)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3198..3253
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3252..3271)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3607..3742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3848..3865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4019..4607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4099..4118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4778..5569,5570..5638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5401..5420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5639..5743)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5811..5829
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5867..5889)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5989..6008
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6127..6145
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6169..6472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg ggggatcgat
      301 ccccccgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
      361 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
      421 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
      481 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
      541 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
      601 aagtactttc tctaatcact ttttttttca aggcaatcag ggtatattat attgtacttc
      661 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
      721 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat
      781 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
      841 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
      901 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgtaatacga
      961 ctcactatag ggcgaattcg gatccctgcc gccaccatgg gacctccctc aactctgcag
     1021 cactactcaa atccccctcc atatagcgag gaagaccagg gaatcgatgg accacagcgg
     1081 agccagccac tgtctacccc tcaccagtac gatcgctact atggcgtgaa cattcagcac
     1141 acacgagtct ataatcatct ggggactatc tactccggac tgaagctggc tttccagatt
     1201 ctgggctggg tgtccgtcat cattacaatg atcattactg tgaccacact gaagaaaatg
     1261 agtgatggga acagccagga ctccgcaatg ctgaagtcac tggacgagaa tttcgatgct
     1321 atccaggaag tggcaaacct gctggacaat gaggtgcgcc ctaaactggg cgtcactatg
     1381 acccagacta cctttcagct gccaaaggag ctgtccgaga tcaagagata cctgctgcga
     1441 ctggagcgaa actgccccgt gtgcggaaca gaagcaactc cacagggatc taagggcaat
     1501 gccagtggag ataccgcttt ctgcccccct tgtctgacac gccagtgctc tgaggacagt
     1561 actcatgatc agggacccgg cgtggaagga acctctcgaa accacaaggg caaaatcaat
     1621 tttcctcata ttctgcagtc cgacgattgt gggcggtctg acaacctgat cgtgtatagt
     1681 attaatctgg tccctggact gagtttcatc cagctgccat caggcacaaa gcactgcatc
     1741 attgatgtga gttatacctt ttcagacaca ctggcaggct acctgattgt gggcggggtc
     1801 gatggatgtc agctgcataa caaagccatc atctacctgt ccctgggcta ctataagacc
     1861 aaaatgatct atccacccga ctacatcgca attgccacat acacttatga tctggtgcct
     1921 aacctgagag actgcagcat cgccgtcaat cagacatccc tggccgctat ttgtacttct
     1981 aagaaaacca aggagaacca ggatttctcc acttctggag tgcacccatt ctatatcttt
     2041 accctgaata cagatggcat ctttaccgtg acagtcattg aacagtctca gctgaagctg
     2101 gactaccagt atgcagccct gtacccagct accggaccag gaatcttcat tggcgaccat
     2161 ctggtgtttc tgatgtgggg aggcctgatg accaaagctg agggcgatgc atattgccag
     2221 gcaagcggat gtaacgacgc acaccgaaca tcatgcaata tcgcacagat gccaagcgcc
     2281 tacgggcatc ggcagctggt gaacggactg ctgatgctgc ccatcaagga gctgggaagc
     2341 cacctgattc agccctcact ggaaacaatc agccctaaga tcaactgggc cgggggacat
     2401 ggcaggctgt actataactg ggagatcaat acaacttaca tctatatcga aggaaagacc
     2461 tggaggtccc gccccaacct gggcatcatt agttggtcaa aacctctgag tatccggtgg
     2521 attgaccact cagtggctcg gagacctggc gcaagaccat gtgactctgc caatgattgc
     2581 cctgaggact gtctggtggg cgggtactat gatatgttcc caatgagctc cgactacaag
     2641 actgcaatca ccatcattcc cactcaccat cagtggcctt ctagtccagc cctgaaactg
     2701 tttaacacca atagagaagt gagggtggtc atgatcctga ggcctccaaa caatgtcaag
     2761 aaaaccacaa tttcctgcat ccgcattatg cagacaaact ggtgtctggg cttcatcatc
     2821 ttcaaggagg gcaacaatgc ttgggggcag atctactcct atatctacca ggtggaatcc
     2881 acttgcccaa atactaaaac ggccgcacgg ggtaccggga gccatcacca tcatcatcac
     2941 tgacgactag tgtgaaatag acatcagaat taagaaaaac gtagggtcca agtggttccc
     3001 cgttatggac tcgctatctg tcaaccagat cttataccct gaagttcacc tagatagccc
     3061 gatagttacc aataagatag tagccatcct ggagtatgct cgactgttta aacctgcagg
     3121 catgcaagct gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     3181 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3241 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     3301 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     3361 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     3421 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     3481 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     3541 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgaggagc tttttgcaaa
     3601 agcctaggcc tccaaaaaag cctcttcact acttctggaa tagctcagag gccgaggcgg
     3661 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     3721 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     3781 tgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5761 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5821 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     5881 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     5941 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     6001 agattgtact gagagtgcac cattcgacgc tctcccttat gcgactcctg cattaggaag
     6061 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tctggcttat
     6121 cgaaattaat acgactcact atagggagac cggaattcga gctcgccccg ttacataact
     6181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     6301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     6361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     6421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tg
//
