LOCUS       sequence-325538-        9636 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses human CPSF6 fused to iRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, 
            Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z
  TITLE     Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection 
            in a Cyclophilin A-Dependent Manner.
  JOURNAL   mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 
            10.1128/mBio.03142-20.
  PUBMED    33758083
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-23"; volume: "12(2). pii: mBio.03142-20. doi"; pages:
            " 10.1128/mBio.03142-20"
COMMENT     Sequence Label: pSICO-CPSF6-iRFP670
FEATURES             Location/Qualifiers
     source          1..9636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2854..2874
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(2892..2925)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        3025..3328
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3329..3532
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5386..5395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5392..6327
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     protein_bind    complement(6374..6407)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     6434..6450
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6525..7113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6578..6598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6996..7007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7116..7132)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7642..7822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7884..8472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7964..7983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8643..9434,9435..9503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9266..9285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9504..9608)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg ccgccatctc
     2641 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc
     2701 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac
     2761 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa
     2821 gagatacaaa tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact
     2881 gtaaagtaat tataacttcg tatagtataa attatacgaa gttataagcc ttggtttttt
     2941 gaattccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     3001 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3061 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3121 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3181 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3241 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3301 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3361 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3421 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3481 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3541 gaaccgtcgg atcccagtgt ggtggtacgg gaattcagga agatggcgga cggcgtggac
     3601 cacatagaca tttacgcgga tgtcggcgaa gagttcaacc aggaagctga atatggtggg
     3661 catgatcaga tagatttgta tgacgatgtc atatctccat ctgcaaataa tggagatgcc
     3721 ccagaagacc gagattacat ggatactctc ccaccaactg ttggtgatga tgtgggtaaa
     3781 ggagcagcac caaatgttgt ctatacatat actggaaaga gaattgcatt atatattgga
     3841 aatctaacat ggtggacaac agatgaagac ttaactgaag cagttcattc tttgggagta
     3901 aatgatattt tggagataaa attttttgaa aatcgggcaa atggccagtc aaaggggttt
     3961 gcccttgttg gtgttggatc tgaagcatct tcaaaaaagt taatggatct gttacctaaa
     4021 agagaacttc atggtcagaa tcctgttgta actccatgca ataaacagtt cctgagtcaa
     4081 tttgaaatgc agtccaggaa aactacacaa tcaggacaaa tgtctgggga aggtaaagct
     4141 ggtcctccag gaggcagttc ccgtgcagca tttccacaag gtggtagagg acggggccgt
     4201 tttccagggg ctgttcctgg tggggacaga tttcctgggc cagcaggacc aggagggcca
     4261 cccccacctt ttccaggaaa tttgatcaag catcttgtta aaggaactcg gcctttgttc
     4321 ctggaaacta ggattccatg gcatatgggg cacagcatag aggaaatacc catttttggc
     4381 ctaaaagctg gacagactcc accacgtcca cccttaggtc ctccaggccc acctggtcca
     4441 ccaggtcctc cacctcctgg tcaggttctg cctcctcctc tagctgggcc tcctaatcga
     4501 ggagatcgcc ctccaccacc agttcttttt cctggacaac cttttgggca gcctccattg
     4561 ggtccacttc ctcctggccc tccacctcca gttccaggct acggcccccc tcctggccca
     4621 ccacctccac aacagggacc acctccacct ccaggcccct ttccacctcg tccacccggt
     4681 ccacttgggc caccccttac actagctcct cctccgcatc ttcctggacc acctccaggt
     4741 gccccaccgc cagctccgca tgtgaaccca gctttctttc ctccaccaac taacagtggc
     4801 atgcctacat cagatagccg aggtccacca ccaacagatc catatgggcg acctccacca
     4861 tatgataggg gtgactatgg cccccctgga agggaaatgg atactgcaag aacgccattg
     4921 agtgaagctg aatttgaaga aatcatgaat agaaataggg caatctcaag cagtgctatt
     4981 tcgagagctg tgtctgatgc cagtgctggt gattatggga gtgctattga gacactggta
     5041 actgcaattt ctttaattaa acaatccaaa gtatctgctg atgatcgttg caaagttctt
     5101 attagttctt tgcaagattg ccttcatgga attgagtcca agtcttatgg ttctggatca
     5161 agacgtgaac gatcaagaga gagggaccat agtagatcac gagaaaagag tcgacgtcat
     5221 aaatcccgta gtagagaccg tcatgacgat tattacagag agagaagcag agaacgagag
     5281 aggcaccggg atcgtgaccg agaccgtgac cgagagcgtg accgagagcg cgaatatcgt
     5341 catcgtgagg taccgcgggc ccgggatccg ctagcgctac cggtcgccac catggcgcgt
     5401 aaggtcgatc tcacctcctg cgatcgcgag ccgatccaca tccccggcag cattcagccg
     5461 tgcggctgcc tgctagcctg cgacgcgcag gcggtgcgga tcacgcgcat tacggaaaat
     5521 gccggcgcgt tctttggacg cgaaactccg cgggtcggtg agctactcgc cgattacttc
     5581 ggcgagaccg aagcccatgc gctgcgcaac gcactggcgc agtcctccga tccaaagcga
     5641 ccggcgctga tcttcggttg gcgcgacggc ctgaccggcc gcaccttcga catctcactg
     5701 catcgccatg acggtacatc gatcatcgag ttcgagcctg cggcggccga acaggccgac
     5761 aatccgctgc ggctgacgcg gcagatcatc gcgcgcacca aagaactgaa gtcgctcgaa
     5821 gagatggccg cacgggtgcc gcgctatctg caggcgatgc tcggctatca ccgcgtgatg
     5881 ttgtaccgct tcgcggacga cggctccggg atggtgatcg gcgaggcgaa gcgcagcgac
     5941 ctcgagagct ttctcggtca gcactttccg gcgtcgctgg tcccgcagca ggcgcggcta
     6001 ctgtacttga agaacgcgat ccgcgtggtc tcggattcgc gcggcatcag cagccggatc
     6061 gtgcccgagc acgacgcctc cggcgccgcg ctcgatctgt cgttcgcgca cctgcgcagc
     6121 atctcgccct gccatctcga atttctgcgg aacatgggcg tcagcgcctc gatgtcgctg
     6181 tcgatcatca ttgacggcac gctatgggga ttgatcatct gtcatcatta cgagccgcgt
     6241 gccgtgccga tggcgcagcg cgtcgcggcc gaaatgttcg ccgacttctt atcgctgcac
     6301 ttcaccgccg cccaccacca acgctaatat cgctgcactt caccgccgcc caccaccaac
     6361 gctaagcggc cgcataactt cgtatagtat aaattatacg aagttataag ccttgttaac
     6421 gcgcggtgac cctcgaggtc gacggtatcg ataagctcgc ttcacgagat catgtttaag
     6481 ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt
     6541 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6601 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6661 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6721 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6781 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6841 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6901 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6961 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7021 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7081 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac
     7141 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7201 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7261 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7321 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7381 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     7441 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     7501 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7561 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     7621 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9601 gttccgcgca catttccccg aaaagtgcca cctgac
//
