LOCUS       sequence-325942-       10422 bp    DNA     circular SYN 15-APR-2025
DEFINITION  CMV-dcas13d-NLS-ADARdd- mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Liu Y, Mao S, Huang S, Li Y, Chen Y, Di M, Huang X, Lv J, Wang X, Ge
            J, Shen S, Zhang X, Liu D, Huang X, Chi T
  TITLE     REPAIRx, a specific yet highly efficient programmable A > I RNA base
            editor.
  JOURNAL   EMBO J. 2020 Nov 16;39(22):e104748. doi: 10.15252/embj.2020104748. 
            Epub 2020 Oct 15.
  PUBMED    33058207
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2020-11-16"; volume: "39(22):e104748. doi"; pages: " 
            10.15252/embj.2020104748. Epub 2020 Oct 15"
COMMENT     Sequence Label: TC1092
FEATURES             Location/Qualifiers
     source          1..10422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             959..979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3923..3943
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5111..5176
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5201..5257
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5264..5974
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     5677..5696
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6013..6030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6019..6243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6289..6717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6376..6395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6586..6607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6726..6746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6731..7060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6911..7046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6973..6992
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7127..7921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7181..7200)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7791..7810
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8095..8216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8132..8151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8186..8205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8265..8281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8265..8281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8278..8300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8289..8305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8313..8319,8320..8337,8338..8343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8358..8379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8496..8513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8667..9255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8747..8766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9426..10217,10218..10286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10049..10068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10287..10391)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgaaacg gactgctgac ggcagcgaat ttgagagccc
      961 caagaagaag aggaaagtcg gatccatcga gaagaagaag agcttcgcca agggcatggg
     1021 agtgaagagc accctggtgt ccggctctaa ggtgtacatg accacatttg ctgagggaag
     1081 cgacgccagg ctggagaaga tcgtggaggg cgatagcatc agatccgtga acgagggaga
     1141 ggctttcagc gccgagatgg ctgacaagaa cgctggctac aagatcggaa acgccaagtt
     1201 ttcccaccca aagggctacg ccgtggtggc taacaaccca ctgtacaccg gaccagtgca
     1261 gcaggacatg ctgggactga aggagacact ggagaagagg tacttcggcg agtccgccga
     1321 cggaaacgat aacatctgca tccaggtcat ccacaacatc ctggatatcg agaagatcct
     1381 ggctgagtac atcacaaacg ccgcttacgc cgtgaacaac atctccggcc tggacaagga
     1441 tatcatcggc ttcggaaagt tttctaccgt gtacacatac gacgagttca aggatccaga
     1501 gcaccaccgg gccgctttta acaacaacga caagctgatc aacgccatca aggctcagta
     1561 cgacgagttc gataactttc tggataaccc caggctgggc tacttcggac aggctttctt
     1621 ttctaaggag ggcagaaact acatcatcaa ctacggaaac gagtgttacg acatcctggc
     1681 cctgctgagc ggactggccc actgggtggt ggccaacaac gaggaggagt ctcggatcag
     1741 ccgcacctgg ctgtacaacc tggacaagaa cctggataac gagtacatct ccacactgaa
     1801 ctacctgtac gacaggatca ccaacgagct gacaaacagc ttctccaaga actctgccgc
     1861 taacgtgaac tacatcgctg agaccctggg catcaaccca gctgagttcg ctgagcagta
     1921 cttcagattt tccatcatga aggagcagaa gaacctgggc ttcaacatca caaagctgag
     1981 agaagtgatg ctggacagaa aggatatgtc cgagatcagg aagaaccaca aggtgttcga
     2041 ttctatcaga accaaggtgt acacaatgat ggactttgtg atctacaggt actacatcga
     2101 ggaggatgcc aaggtggccg ctgccaacaa gagcctgccc gacaacgaga agtctctgag
     2161 cgagaaggat atcttcgtga tcaacctgag aggctccttt aacgacgatc agaaggacgc
     2221 tctgtactac gatgaggcca acaggatctg gagaaagctg gagaacatca tgcacaacat
     2281 caaggagttc cggggaaaca agacccgcga gtacaagaag aaggacgctc caaggctgcc
     2341 taggatcctg cctgctggaa gggacgtgag cgccttcagc aagctgatgt acgccctgac
     2401 aatgtttctg gacggaaagg agatcaacga tctgctgacc acactgatca acaagttcga
     2461 caacatccag tcttttctga aagtgatgcc tctgatcggc gtgaacgcta agttcgtgga
     2521 ggagtacgcc ttctttaagg acagcgccaa gatcgctgat gagctgcggc tgatcaagtc
     2581 ctttgccagg atgggagagc caatcgctga cgctaggaga gctatgtaca tcgatgccat
     2641 ccggatcctg ggaaccaacc tgtcttacga cgagctgaag gctctggccg acaccttcag
     2701 cctggatgag aacggcaaca agctgaagaa gggcaagcac ggaatgcgca acttcatcat
     2761 caacaacgtg atcagcaaca agcggtttca ctacctgatc agatacggcg acccagctca
     2821 cctgcacgag atcgctaaga acgaggccgt ggtgaagttc gtgctgggac ggatcgccga
     2881 tatccagaag aagcagggcc agaacggaaa gaaccagatc gaccgctact acgagacctg
     2941 catcggcaag gataagggaa agtccgtgtc tgagaaggtg gacgctctga ccaagatcat
     3001 cacaggcatg aactacgacc agttcgataa gaagagatct gtgatcgagg acaccggaag
     3061 ggagaacgcc gagagagaga agtttaagaa gatcatcagc ctgtacctga cagtgatcta
     3121 ccacatcctg aagaacatcg tgaacatcaa cgctagatac gtgatcggct tccactgcgt
     3181 ggagcgcgat gcccagctgt acaaggagaa gggatacgac atcaacctga agaagctgga
     3241 ggagaagggc tttagctccg tgaccaagct gtgcgctgga atcgacgaga cagcccccga
     3301 caagaggaag gatgtggaga aggagatggc cgagagagct aaggagagca tcgactccct
     3361 ggagtctgct aaccctaagc tgtacgccaa ctacatcaag tactccgatg agaagaaggc
     3421 cgaggagttc accaggcaga tcaacagaga gaaggccaag accgctctga acgcctacct
     3481 gaggaacaca aagtggaacg tgatcatccg ggaggacctg ctgcgcatcg ataacaagac
     3541 ctgtacactg ttcgctaaca aggctgtggc cctggaggtg gctcgctacg tgcacgccta
     3601 catcaacgac atcgccgagg tgaactccta ctttcagctg taccactaca tcatgcagag
     3661 gatcatcatg aacgagagat acgagaagtc tagcggcaag gtgtctgagt acttcgacgc
     3721 cgtgaacgat gagaagaagt acaacgatag actgctgaag ctgctgtgcg tgcctttcgg
     3781 atactgtatc ccacggttta agaacctgag catcgaggcc ctgttcgacc gcaacgaggc
     3841 tgccaagttt gataaggaga agaagaaggt gagcggcaac tccggttcta agcgcaccgc
     3901 cgatggatcc gagttcgaat cccctaaaaa gaaaagaaag gtgggatccc agctgcattt
     3961 accgcaggtt ttagctgacg ctgtctcacg cctggtcctg ggtaagtttg gtgacctgac
     4021 cgacaacttc tcctcccctc acgctcgcag aaaagtgctg gctggagtcg tcatgacaac
     4081 aggcacagat gttaaagatg ccaaggtgat aagtgtttct acaggaacaa aatgtattaa
     4141 tggtgaatac atgagtgatc gtggccttgc attaaatgac tgccatgcag aaataatatc
     4201 tcggagatcc ttgctcagat ttctttatac acaacttgag ctttacttaa ataacaaaga
     4261 tgatcaaaaa agatccatct ttcagaaatc agagcgaggg gggtttaggc tgaaggagaa
     4321 tgtccagttt catctgtaca tcagcacctc tccctgtgga gatgccagaa tcttctcacc
     4381 acatgagcca atcctggaag aaccagcaga tagacaccca aatcgtaaag caagaggaca
     4441 gctacggacc aaaatagagt ctggtcaggg gacgattcca gtgcgctcca atgcgagcat
     4501 ccaaacgtgg gacggggtgc tgcaagggga gcggctgctc accatgtcct gcagtgacaa
     4561 gattgcacgc tggaacgtgg tgggcatcca gggatcactg ctcagcattt tcgtggagcc
     4621 catttacttc tcgagcatca tcctgggcag cctttaccac ggggaccacc tttccagggc
     4681 catgtaccag cggatctcca acatagagga cctgccacct ctctacaccc tcaacaagcc
     4741 tttgctcagt ggcatcagca atgcagaagc acggcagcca gggaaggccc ccaacttcag
     4801 tgtcaactgg acggtaggcg actccgctat tgaggtcatc aacgccacga ctgggaagga
     4861 tgagctgggc cgcgcgtccc gcctgtgtaa gcacgcgttg tactgtcgct ggatgcgtgt
     4921 gcacggcaag gttccctccc acttactacg ctccaagatt accaagccca acgtgtacca
     4981 tgagtccaag ctggcggcaa aggagtacca ggccgccaag gcgcgtctgt tcacagcctt
     5041 catcaaggcg gggctggggg cctgggtgga gaagcccacc gagcaggacc agttctcact
     5101 cacgggatcc gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa
     5161 ggatgacgat gacaagggat ccaaaagaac tggaagcgga gctactaact tcagcctgct
     5221 gaagcaggct ggagacgtgg aggagaaccc tggacctgtt aacatggtga gcaagggcga
     5281 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     5341 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5401 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     5461 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     5521 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     5581 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     5641 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     5701 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     5761 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     5821 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     5881 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     5941 ctccaccggc ggcatggacg agctgtacaa gtaagctcga gcctagaggg cccgtttaaa
     6001 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6061 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6121 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6181 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6241 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6301 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6361 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6421 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6481 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6541 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6601 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6661 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6721 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6781 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6901 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6961 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7021 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     7081 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7141 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7201 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7261 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7321 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7381 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7441 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7501 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7561 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7621 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7681 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7741 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7801 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7861 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7921 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8221 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
