LOCUS       sequence-32626-e         874 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Held M, Schmitz MH, Fischer B, Walter T, Neumann B, Olma MH, Peter 
            M, Ellenberg J, Gerlich DW
  TITLE     CellCognition: time-resolved phenotype annotation in high-throughput
            live cell imaging.
  JOURNAL   Nat Methods. 2010 Sep . 7(9):747-54.
  PUBMED    20693996
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Sep . 7(9):747-54."
COMMENT     Sequence Label: pIBB-mEGFP-IRES-puro2b
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             213..224
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(294..497)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(323..343)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        498..801
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgcccttgc tgttcatggg agaagtagca tcatcaggaa atgagcttac atttctcctc
       61 ttcagcatct ggtcatcctt cttagctttc ctcagctcca cattgacctc tattctgtga
      121 cgcctcattt ctgtactgtc ttttcccttg ttcttgaatc tgtgaagacg ggcagctggt
      181 gtattagcat tctcgttcgt gcgcatgctt aatttctcct ctttaatgaa ttcgaagctt
      241 gagctcgaga tctgagtccg gtagcgctag cggatctgac ggttcactaa accagctctg
      301 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      361 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      421 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      481 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      541 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
      601 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
      661 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
      721 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
      781 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaaacta atgaccccgt
      841 aattgattac tattaataac tagtcaataa tcaa
//
