LOCUS       sequence-326572-       29350 bp    DNA     circular UNA 14-APR-2025
DEFINITION  Episomal expression of reprogramming factors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 29350)
  AUTHORS   Yoshimatsu S, Qian E, Sato T, Yamamoto M, Ishikawa M, Okano H
  TITLE     Establishing an induced pluripotent stem cell line from neonatal 
            common marmoset fibroblasts by an all-in-one episomal vector 
            approach.
  JOURNAL   Stem Cell Res. 2021 May;53:102380. doi: 10.1016/j.scr.2021.102380. 
            Epub 2021 Apr 30.
  PUBMED    34088009
REFERENCE   2  (bases 1 to 29350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2021-05"; volume: "53:102380. doi"; pages: " 
            10.1016/j.scr.2021.102380. Epub 2021 Apr 30"
COMMENT     Sequence Label: pMasterE
FEATURES             Location/Qualifiers
     source          1..29350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    87..134
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1883..1939)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(1940..2536)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2020..2040)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2517..2536
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(2537..2590)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(2591..3301,3302..3304,3305..3307))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2591..3301,3302..3304,3305..3307))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2982..3001
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3304..3313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3325..4503)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3334..4272
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     polyA_signal    4524..4571
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4803..5606)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4914..4933)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5524..5543
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    join(5607..5615,5616..5618,5619..6193)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5780..5799)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6009..6026
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6209..7339)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     intron          7412..8428
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     enhancer        8707..9010
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      9136..9591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9223..9242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9433..9454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    9598..9719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9843..11162)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             complement(11163..11222)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(12183..12236)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(13656..13721)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     regulatory      14807..14816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(14824..16002)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          14833..15771
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     enhancer        16109..16412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          16693..17709
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     polyA_signal    18704..18825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      19304..21093
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     gap             22869..22888
                     /estimated_length=20
     primer_bind     complement(23767..23785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        23853..23957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(23958..24026,24027..24818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(24176..24195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      24989..25577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     25478..25497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(26694..26759)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     intron          27752..28768
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     enhancer        join(29046..29350,1..75)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
       61 tcaataatca atgtcgaggg cgcgccgaac aaacgaccca acaccgtgcg ttttattctg
      121 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
      181 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
      241 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
      301 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
      361 cccgcggccg cgtcgacatt taaatttatg ctcttttggc atgcaacatt tcaatgagaa
      421 ggttattata gggcacatcc ccgttcaggt gcttgtagta gaggtattct tcagcctgca
      481 tactgatggc ccggatttcg ggtagtcgaa gaagtagctg tccaaatttc tctgtctgct
      541 gcgggtagtt acacattgtg tagtccagca gggcggcatt gacttgttcc tggacacctt
      601 ctaccagctg gaagttttca aggtttttga catctaaact aaagagcacc aagaatttca
      661 gacatacgaa ctctcgttga tcaaactgga gagaacgaag ttttgccact aactcctgtg
      721 catgactcat gaggttgttg agggtggctc cggcttgtga tgctattatg gaatagtcca
      781 cttgttgccc agtaaccagg aagatggatc cttcctttcc atgtaccact tgtcggtaaa
      841 tgtggtcgag gattaagagc tcactccagc agttctgaag cagcttcatt tggtcatcaa
      901 ccttaagttc tctgaagaag atactactcc tggcccactc gacaatggag aagagagttt
      961 gatctgccat tttgcacata agcccaaagg tgctcagctt ttcgtgcttg cttcggttag
     1021 cctgctcttg ctgcaaatag gccatgattt tagcctggac ttgaggctca tctggctcac
     1081 acttcaaaag ttccagtatc agatgtggga tgcttgctgg agagctcgtc tggtaactat
     1141 ccatatatga atagcccatt atggactcgg gtgagctggt ataggggtct gggtactcag
     1201 acttgatggc ccggctagga aagtggccat atgtttggta accttgcagg ctgccgtgag
     1261 ggggcattgt catgctaatg ggggatgtta caaagggact tctgtcatag tctgtaggag
     1321 gcaaggcagc atggttcaga ggtaggcctt tggaggcaga gtggatgttt tgaattgcag
     1381 aggaaatggt caggtcagag ggcatagctt ggatcacctg agacatggct tctagcttaa
     1441 gtccattggc tcggatgagg gcttttttct gttgcttcag ggccctgtct ctcttgtaca
     1501 ttggcccaaa cttattcctt cctccacgca ttcggtcggc ccttacagct tctagcttca
     1561 ttccaacact tagacatttt tgaaaacgac agtaaggaca acgctttctc tgtgttttgt
     1621 caatttggca gttctggttt tctatacatg tgtacctttt attattttgg actgttcgct
     1681 taaaaaatcc cttgcagctt tcacaggtga ggagcccata atggtaccca gacactttat
     1741 ctccacacac gggacaaagc tcttccagat cttcatcata ggagtaattc accattttaa
     1801 attgagacac aataggtgta agtccgtgct ttaaagactc ttgtaaatcc ccagtatctg
     1861 aattagaaga catggtggcg ataggaccgg ggttttcttc cacgtctcct gcttgcttta
     1921 acagagagaa gttcgtggcg gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     1981 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     2041 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     2101 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     2161 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     2221 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     2281 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
     2341 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
     2401 cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
     2461 cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
     2521 gcttgtactc ggtcatgggg ccgggatttt cctccacgtc cccgcatgtt agaagacttc
     2581 ccctgccctc tttatatagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     2641 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     2701 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     2761 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     2821 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     2881 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     2941 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     3001 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     3061 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     3121 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     3181 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     3241 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     3301 tcaccatggt ggcgatcgct agcttcacga cacctgaaat ggaagaaaaa aactttgaac
     3361 cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga
     3421 gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa
     3481 ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa
     3541 ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga
     3601 ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg
     3661 cgtcctccat tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc
     3721 aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag
     3781 gccaggcacc agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg
     3841 cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc
     3901 cgcggcccca aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc
     3961 ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc
     4021 gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc
     4081 aggccaggcc tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact
     4141 ctcccaccca cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga
     4201 agtaattcaa ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca
     4261 cacggcactt acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac
     4321 tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac
     4381 atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc
     4441 tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga
     4501 gcctcacgat taatcgccgc gccgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     4561 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     4621 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     4681 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     4741 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     4801 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     4861 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     4921 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     4981 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     5041 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     5101 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     5161 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5221 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5281 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5341 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5401 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5461 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5521 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5581 ccatcttgtt caatggcgga tcccatggtt gtggccatat tatcatcgtg tttttcaaag
     5641 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca
     5701 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
     5761 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     5821 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     5881 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
     5941 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
     6001 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
     6061 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
     6121 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg
     6181 ggagggagag gggcggatcc gcggccgctc agttagcctc ccccatctcc cgggcaaacg
     6241 tgcgcgccag gtcgcatatc gtcggtatgg agccgggggt ggtgacgtgg gtctggacca
     6301 tcccggaggt aagttgcagc agggcgtccc ggcagccggc gggcgattgg tcgtaatcca
     6361 ggataaagac gtgcatggaa cggaggcgtt tggccaagac gtccaaggcc caggcaaaca
     6421 cgttatacag gtcgccgttg ggggccagca actcgggggc ccgaaacagg gtaaataacg
     6481 tgtccccgat atggggtcgt gggcccgcgt tgctctgggg ctcggcaccc tggggcggca
     6541 cggccgtccc cgaaagctgt ccccagtcct cccgccacga cccgccgcac tgcagatacc
     6601 gcaccgtatt ggcaagtagc ccgtaaacgc ggcgaatcgc agccagcata gccaggtcca
     6661 gccgctcgcc ggggcgctgg cgtttggcca ggcggtcgat gtgtctgtcc tccggaaggg
     6721 ccccaagcac gatgttggtg ccgggcaagg tcggcgggat gagggccacg aacgccagca
     6781 cggcctgggg ggtcatgctg cccataaggt accgcgcggc cgggtagcac aggagggcgg
     6841 cgatgggatg gcggtcgaag atgagggtga gggccggggg cggggcatgt gagctcccag
     6901 cctccccccc gatatgagga gccagaacgg cgtcggtcac ggcataaggc atgcccattg
     6961 ttatctgggc gcttgtcatt accaccgccg cgtccccggc cgatatctca ccctggtcga
     7021 ggcggtgttg tgtggtgtag atgttcgcga ttgtctcgga agcccccagc acccgccagt
     7081 aagtcatcgg ctcgggtacg tagacgatat cgtcgcgcga acccagggcc accagcagtt
     7141 gcgtggtggt ggttttcccc atcccgtggg gaccgtctat ataaacccgc agtagcgtgg
     7201 gcattttctg ctccgggcgg acttccgtgg cttcttgctg ccggcgaggg cgcaacgccg
     7261 tacgtcggtt gctatggccg cgagaacgcg cagcctggtc gaacgcagac gcgtgttgat
     7321 ggccggggta cgaagccata ttaccctgtt atccctagaa ttctttgcca aaatgatgag
     7381 acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat
     7441 ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc
     7501 gtcccccccg aaggcagccg tccccccgcg gacagccccg aggctggaga gggagaaggg
     7561 gacggcggcg cggcgacgca cgaaggccct ccccgcccat ttccttcctg ccggcgccgc
     7621 accgcttcgc cccgcgcccg ctagaggggg tgcggcggcg cctcccagat ttcggctccg
     7681 cacagatttg ggacaaagga agtccctgcg ccctctcgca cgattaccat aaaaggcaak
     7741 ggctgcggct cgccgcgcct cgacagccgc cggcgctccg ggggccgccg cgccccyccc
     7801 ccgrgccctc cccggcccgr ggcggccccg ccccgcccgg cacccccacc tgccgccacc
     7861 ccccgcccgg cacggcgagc cccgcgccac gccccgtacg gagccccgca cccgaagccg
     7921 ggccgtgctc agcaactcgg ggaggggggt gcaggggggg ttgcagcccg accgacgcgc
     7981 ccacaccccc tgctcacccc cccacgcaca caccccgcac gcagcctttg ttcccctcgc
     8041 agcccccccc gcaccgcggg gcaccgcccc cggccgcgct cccctcgcgc acactgcgga
     8101 gcgcacaaag ccccgcgccg cgcccgcagc gctcacagcc gccgggcagc gcggagccgc
     8161 acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc cccccgcaca
     8221 aagggccctc ccggagcccc tcaaggcttt cacgcagcca cagaaaagaa acaagccgtc
     8281 attaaaccaa gcgctaatta cagcccggag gagaagggcc gtcccgcccg ctcacctgtg
     8341 ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga ggcggcggcg gagcggggca
     8401 cggggcgaag gcagcgcgca gcgactcccg cccgccgcgc gcytcscttt ttatrgggcc
     8461 sccsccsccc cccccccvcc awaaaarrmm mmyyycggsg cscccccccc ygkkkgsccc
     8521 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc ccccccggcg
     8581 cscccccccc ccccccccng cccccatcgc tgcacaaaat aattaaaaaa taaataaata
     8641 caaaattggg ggtggggagg ggggggagat ggggagagtg aagcagaacg tggggctcac
     8701 ctcgaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     8761 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     8821 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     8881 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     8941 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     9001 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     9061 ttaataacta gcgctgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9121 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     9181 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     9241 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     9301 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     9361 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     9421 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     9481 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9541 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tacgccttaa
     9601 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     9661 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     9721 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     9781 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
     9841 tattacgcac aagagttccg tagctgttca agtttgtgtt tcaactgttc tcgtcgtttc
     9901 cgcaacaagt cctcttcaga aatgagcttt tgctcctctg cttggacgga caggatgtat
     9961 gctgtggctt ttttaaggat aactaccttg ggggcctttt cattgttttc caactccggg
    10021 atctggtcac gcagggcaaa aaagctccgt tttagctcgt tcctcctctg gcgctccaag
    10081 acgttgtgtg ttcgcctctt gacattctcc tcggtgtccg aggacctggg gctggtgcat
    10141 tttcggttgt tgctgatctg tctcaggact ctgacactgt ccaacttgac cctcttggca
    10201 gcaggatagt ccttccgagt ggagggaggc gctgcgtagt tgtgctgatg tgtggagacg
    10261 tggcacctct tgaggaccag tgggctgtga ggaggtttgc tgtggcctcc agcagaaggt
    10321 gatccagact ctgacctttt gccaggagcc tgcctctttt ccacagaaac aacatcgatt
    10381 tcttcctcat cttcttgttc ctcctcagag tcgctgctgg tggtgggcgg tgtctcctca
    10441 tggagcacca ggggctcggg gctgccctgc ggggaggact ccgtcgagga gagcagagaa
    10501 tccgaggacg gagagaaggc gctggagtct tgcgaggcgc aggacttggg cgagctgctg
    10561 tcgttgagag ggtaggggaa gaccaccgag gggtcgatgc actctgaggc ggcggcgctc
    10621 agatcctgca ggtacaagct ggaggtggag cagacgctgt ggccgcgggc ggggttcggg
    10681 ctgccgctgt ctttgcgcgc agcctggtag gaggccagct tctctgagac gagcttggcg
    10741 gcggccgaga agccgctcca catacagtcc tggatgatga tgtttttgat gaaggtctcg
    10801 tcgtccgggt cgcagatgaa actctggttc accatgtctc ctcccagcag ctcggtcacc
    10861 atctccagct ggtcggccgt ggagaagctc ccgccaccgc cgtcgttgtc tccccgaagg
    10921 gagaagggtg tgaccgcaac gtaggagggc gagcagagcc cggagcggcg gctaggggac
    10981 aggggcgggg tgggcagcag ctcgaatttc ttccagatat cctcgctggg cgccgggggc
    11041 tgcagctcgc tctgctgctg ctgctggtag aagttctcct cctcgtcgca gtagaaatac
    11101 ggctgcaccg agtcgtagtc gaggtcatag ttcctgttgg tgaagctaac gttgaggggc
    11161 ataggaccgg ggttactttc aacatcgcca gcgagtttca acaaagcgta gttagtacat
    11221 tgcccactac ccatgtgtga gaggggcagt gtgccgttaa tggccgtgcc gggcaccggg
    11281 ccgctctggt agtgctggga catgtgaagt ctgctggggg cggcgggttc cggcacctcg
    11341 gcgccgggga gatacatgct gatcatgtcc cggaggtccc cggcctggca gggcgccctg
    11401 gagtgggagg aagaggtaac cacagggggg ctggagctgg cctcggactt gaccaccgaa
    11461 cccatggagc caagagccat gccaggggtg ccctgctgcg agtaggacat gctgtaggtg
    11521 ggcgagccgt tcatgtaggt ctgcgagctg gtcatggagt tgtactgcag ggcgctcacg
    11581 tcgtagcggt gcatgggctg catctgcgct gcgccgtgcg cattgaggcc cgggtgctgc
    11641 gggtagccca gctggtcctg catcatgctg tagctgccgt tgctccagcc gttcatgtgc
    11701 gcgtaactgt ccatgcgctg gttcacgccc gcgcccaggc cggcgcccac cccgaccccg
    11761 ctcgccatgc tattgccgcc gggggccagc agcccgccgg gcagcgtgta cttatccttc
    11821 ttcatgagcg tcttggtttt ccgccggggc cggtatttat aatccgggtg ctccttcatg
    11881 tgcagcgctc gcagccgctt agcctcgtcg atgaacggcc gcttctccgt ctccgacaaa
    11941 agtttccact cggcgcccag gcgcttgctg atctccgagt tgtgcatctt ggggttctcc
    12001 tgggccatct tgcgccgctg cccgcgggac cacaccatga aggcattcat gggccgcttg
    12061 acgcggtccg ggctgttttt ctggttgccg ccggccgccg ccgcggtgga gttgccgccg
    12121 ccgccccccg aagtttgctg cgggcccggc ggcttcagct ccgtctccat catgttgtac
    12181 atggggccgg ggttctcctc cacgtcgccg caggtcagca ggctgcccct gccctcgccg
    12241 ctgccaaaat gcctcttcat gtgtaaggcg aggtggtccg acctggaaaa tgctcggtcg
    12301 catttttggc actggaacgg gcggtgcccc gtgtgtttac ggtagtgcct ggtcagttca
    12361 tctgagcggg cgaatttcca tccacagccg tcccagtcac agtggtaagg tttctcacct
    12421 gtgtgggttc gcaggtgtgc cttgagatgg gaactctttg tgtaggtttt gccgcagccc
    12481 gcgtaatcac aagtgtgggt ggcggtcctt ttccggggcc acgatcgtct tcccctcttt
    12541 ggcttgggct cctctggcat gcaggaaccg ggtggcatga gctcttggta atggagcggc
    12601 gggacttgcg gctgcatctg atcgggcagg aaggatgggt aattgggccc cgggtgggga
    12661 tggaagccgg gaggaagcgg cagggcaggg tgacagtccc tgctgctcag cacttcctca
    12721 agacccaggg tcggggtagt cctgctgggg agctgccgcc ccagggggaa gtcgtgtgca
    12781 gccggccggt ggccattgct gagagggggt ccagcgccca agtgggtgca cgaagagacc
    12841 gcctcctgct tgatcttggg gcacgtgcgc ggcggcccgc cgttgtaggg cgccaccacc
    12901 accgggtggc tgccgtcagg gctgcctttg ctgacgctga tgaccgacgg gctgccgtac
    12961 tcgctgccag gggcgctcag cgacgccttc agcacgaact tgcccatcag cccgccacct
    13021 ggcggctgcg gctgctgcgg cggaatgtac accgggtcca attctggccg caggagctcg
    13081 gccacgaagc cgcccgaggg gctcacgtcg ttgatgtccg ccaggttgaa gggagccgtc
    13141 ggagggggag cggactccct gccatagagg aggcctccgc ccgtgccgcc cggcgccacg
    13201 cccgggtcgt tcccggcccg gatcggatag gtgaagctgc aggtggaggg cgcgctggca
    13261 gggccgctgc tcgacggcga cgacgaagag gaggctgacg ctgacgagga cacggtggcg
    13321 gccactgact ccggaggatg ggtcagcgaa ttggagagaa taaagtccag gtccaggaga
    13381 tcgttgaact cctcggtctc tctccgaggt aggggcgcca ggttgctacc gccgcaagcc
    13441 gcaccggctc cgccgctctc caggtctgtg gccacggtcg ccgccgccag gtcatagggg
    13501 cggccgggaa gcactggggg aagtcgcttc atgtgggaga gctcctcccg ccagcggtta
    13561 ttcggggcac ctgcttgacg cagtgtcttc tcccttcccg ccgggccaga cgcgaacgtg
    13621 gagaaagatg ggagcagcgc gtcgctgaca gccatgggcc ctgggttgga ctccacgtct
    13681 cccgccaact tgagaaggtc aaaattcaaa gtctgtttca cgccacttcc gtttgaatgc
    13741 atgggagagc ccagagtggt gacggagaca gggggaaagg cttccccctc agggaaaggg
    13801 accgaggagt acagtgcagt gaagtgaggg ctcccatagc ctggggtacc aaaatggggc
    13861 cctggggcca gaggaaagga cactggtccc cctgagaaag gagacccagc agcctcaaaa
    13921 tcctctcgtt gtgcatagtc gctgcttgat cgcttgccct tctggcgccg gttacagaac
    13981 cacactcgga ccacatcctt ctcgagccca agctgctggg cgatgtggct gatctgctgc
    14041 agtgtgggtt tcgggcactg caggaacaaa ttctccaggt tgcctctcac tcggttctcg
    14101 atactggttc gctttctctt tcgggcctgc acgagggttt ctgctttgca tatctcctga
    14161 agattttcat tgttgtcagc ttcctccacc cacttctgca gcaagggccg cagcttacac
    14221 atgttcttga agctaagctg cagagcctca aagcggcaga tggtcgtttg gctgaatacc
    14281 ttcccaaata gaacccccag ggtgagcccc acatcggcct gtgtatatcc cagggtgatc
    14341 ctcttctgct tcaggagctt ggcaaattgc tcgagttctt tctgcagagc tttgatgtcc
    14401 tgggactcct ccgggttttg ctccagcttc tccttctcca gcttcacggc accaggggtg
    14461 acggtgcagg gctccgggga ggccccatcg gagttgctct ccaccccgac tcctgcttcg
    14521 ccctcaggct gagaggtctc caagccgcct tggggcacta gccccactcc aacctggggc
    14581 ccacagtacg ccatcccccc acagaactca tacggcgggg ggcatggggg aatcccccac
    14641 acctcagagc ctggcccaac ccccggcccg attcctggcc ctccaggagg gccttggaag
    14701 cttagccagg tccgaggatc aacccagccc ggctccggcc cccctggccc atcacctcca
    14761 ccacctggag ggggcgagaa ggcgaaatcc gaagccaggt gtcccgccat ggtggcgcta
    14821 gcttcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat
    14881 gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc
    14941 tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc
    15001 cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg
    15061 cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct
    15121 ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc
    15181 tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag
    15241 ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg
    15301 gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc
    15361 cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat
    15421 accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc
    15481 agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg
    15541 aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc
    15601 acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg
    15661 aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg
    15721 gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta cctgtgttct
    15781 ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc
    15841 tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc
    15901 gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac
    15961 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctcacgatt aattaatagg
    16021 gataacaggg taatttaaat agatctacta gttattaata gtaatcaatt acggggtcat
    16081 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
    16141 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
    16201 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
    16261 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
    16321 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
    16381 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
    16441 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
    16501 tttgtgcrgs grkgggggsg gggggggggg gggggnncgc gcncaggsgg ggcggggcgg
    16561 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
    16621 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
    16681 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
    16741 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
    16801 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
    16861 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
    16921 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
    16981 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
    17041 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
    17101 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
    17161 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
    17221 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
    17281 ccgggcgggg cggggccgcc ycgggccggg gggggcycgg gggrggggcg cggcggcccc
    17341 cggrgcgccg gcggctgtcg rggcgcggcg rgccgcagcc attgcctttt atggtaatcg
    17401 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
    17461 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
    17521 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
    17581 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
    17641 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
    17701 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
    17761 ttcgctagtc gacgagcggc cgcagctcta aatactctga agtccaaaga agttgtatgt
    17821 tgggtgggct cccttcaact ttaacatgga agtgctttct gtgactttaa aagtaagtgc
    17881 ttccatgttt tagtaggagt gaatccaatt tacttctcca aaatagaaca cgctaacctc
    17941 atttgaaggg atcccctttg ctttaacatg ggggtacctg ctgtgtgaaa caaaagtaag
    18001 tgcttccatg tttcagtgga ggtgtctcca agccagcaca ccttttgtta caaaattttt
    18061 ttgttattgt gttttaaggt tactaagctt gttacaggtt aaaggattct aactttttcc
    18121 aagactgggc tccccaccac ttaaacgtgg atgtacttgc tttgaaacta aagaagtaag
    18181 tgcttccatg ttttggtgat ggtaagtctt ccttttacat ttttattatt tttttagaaa
    18241 ataactttat tgtattgacc gcagctcata tatttaagct ttattttgta tttttacatc
    18301 tgttaagggg ccccctctac tttaacatgg aggcacttgc tgtgacatga caaaaataag
    18361 tgcttccatg tttgagtgtg gtggttccta cctaatcagc aattgcgtta acgcccacac
    18421 tgtgtgcagt tcttggctac aggccattac tgttgctaat atgcaactct gttgaatata
    18481 aattggaatt gcactttagc aatggtgatg gattgttaag ccaatgacag aatttaaacc
    18541 acagacttac tttgatagca ctcttaatgg tataacttct tctcccaggc gcgtctagat
    18601 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    18661 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    18721 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    18781 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagac gtctttaatt
    18841 aatcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
    18901 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
    18961 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
    19021 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
    19081 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
    19141 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
    19201 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
    19261 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
    19321 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
    19381 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
    19441 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
    19501 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
    19561 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
    19621 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
    19681 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
    19741 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
    19801 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
    19861 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
    19921 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
    19981 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
    20041 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
    20101 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
    20161 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
    20221 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
    20281 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
    20341 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
    20401 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
    20461 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    20521 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
    20581 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
    20641 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
    20701 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
    20761 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
    20821 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
    20881 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
    20941 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
    21001 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
    21061 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
    21121 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
    21181 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
    21241 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
    21301 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
    21361 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
    21421 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
    21481 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
    21541 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
    21601 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
    21661 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
    21721 taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct
    21781 tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta
    21841 cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg
    21901 aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg cctccatcac
    21961 cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg ccctgctcta
    22021 tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg tattcaaaat
    22081 aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc ctgcgcggtg
    22141 gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct ccacgtccac
    22201 gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt cctctacccc
    22261 ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc cggcctccac
    22321 tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct scccctcctc
    22381 ctcctcctcc ccctcccccy ccyccycccc ccccycctcc cccccctccc cctccccccc
    22441 ccccyccccc cccccccccc ccycccccyc cccccccccc ycctcctccs ccccctccyc
    22501 ccccyccycc tccyccycct cctccccctc ctcctcctcc ccctcctccc cctcctcctc
    22561 ctccccctcc tccccctcct cctcctcccc ctcctccnnt cctgcccctc ctscnccync
    22621 cctcctgctc ctgcccctcc tgcccctcct gcycctscyc ctcctscccc ccctcctscy
    22681 cctcctccyc ctcctccycc tcctccnnnt cytcsccctc cscctcctcs tccyccctct
    22741 cctccccccc ctccccccca ctmcccctcc ccckcccccc ccccctcccc ccccccyccc
    22801 scyccccccc ctcctscysc ncctscycct cctsyykctc ctgcccctcc tcctsctcct
    22861 cccctcacnn nnnnnnnnnn nnnnnnnncc cccctccccc cccccnnnnn csscccycyc
    22921 cycmcccgcc acctccycct cctccccmcc ctcctcctcc tscncctcct sccmcwccyc
    22981 cycctcctsc ycctcctccy cctcctgccc ctcctcctsc tcctgcccct cctgctcctg
    23041 cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac
    23101 ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc
    23161 ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt
    23221 tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc
    23281 ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca
    23341 ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga
    23401 ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga
    23461 tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc
    23521 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
    23581 tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc
    23641 tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt
    23701 gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
    23761 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
    23821 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    23881 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    23941 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    24001 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    24061 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    24121 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    24181 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
    24241 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    24301 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    24361 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    24421 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    24481 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    24541 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    24601 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    24661 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    24721 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    24781 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    24841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    24901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    24961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    25021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    25081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    25141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    25201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    25261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    25321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    25381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    25441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    25501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    25561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    25621 ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
    25681 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
    25741 gcgtcgacgt ttatcttatc aattctgtgc ctccgggagc agggtagggc tgtggatttc
    25801 ttcttcttcc tctcgaaagt aggttggctt tccctgtgca ctagggccct gctgggcctt
    25861 cagcggacat gaggctacca tatggctgat gctctggcag awktkrcrgt tcctggcctt
    25921 ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
    25981 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
    26041 gcctcgcgtc gacgtttatc ttatcaattc tgtgcctccg ggagcagggt agggctgtgg
    26101 atttcttctt cttcctctcg aaagtaggtt ggctttccct gtgcactagg gccctgctgg
    26161 gccttcagcg gacatgaggc taccatatgg ctgatgctct ggcagaagtg gcacttcttg
    26221 ggctggggtg gcagcttgca ttccttggca tgatgatcta gacctccaca gttgtagcac
    26281 ctgtctcctt ttgatctgcg cttctgcatg ctctttcctt ttggccgcct ctcactccca
    26341 atacagaata ctccaccagg tccggtgaca cggatggatt ccagaccctt ggctgacttc
    26401 ttaaaggtga actccactgc ctcaccctcc ttcaagctcc ggaacccttc catgtgcagc
    26461 ttactctggt gcacaaagac atccactggg gggtcgagcg cgaccccggc gcgggcggtc
    26521 atggacagga agccgaaccc catgcgcacg ttgaaccact tacagatgcc cgcaccgtgc
    26581 agcagctgag gctcgtccgc cgcccgggcc gcgtcctccg gcgcctcctc gggcgcctct
    26641 tctgccgcct tggcgcagcc acctgcaaac tgctggttgg acacggagcc catgggccct
    26701 gggttggact ccacgtctcc cgccaacttg agaaggtcaa aattcaaagt ctgtttcacg
    26761 ccacttccca cgtcttcagg ttgcatgttc atggagtagt ttaggaataa atccatggtt
    26821 tgtggagtac taaaatacct agtggtctgc tgtattacat taaggccttc cccagcagct
    26881 tccaaggcag cctccaagtc actggcagga gaatttggct ggaactgcat gcaggactgc
    26941 agagattcct ctccacagtt atagaaggga ctgttccagg cctgattgtt ccaggattgg
    27001 gtgcaccagg tctgagtgtt ccaggagtgg ttgctccagg actggatgtt ctgggtctgg
    27061 ttgctccagg ttgaattgtt ccaggtctgg ttgctccaca ttggaaggtt cccagtcggg
    27121 ttcaccaggc atccctggtg gtaggaagag taaaggctgg ggtaggtagg tgctgaggcc
    27181 ttctgcgtca caccattgct attcttcggc cagttgtttt tctgccacct cttagatttc
    27241 attctctggt tctggaacca ggtcttcacc tgtttgtagc tgaggttcag gatgttggag
    27301 agttcttgca tctgctggag gctgaggtat ttctgtctct gaaatctatc attgagtaca
    27361 cacagctggg tggaagagaa cacagttctg gtcttctgtt tcttgaccgg gaccttgtct
    27421 tccttttttg cgacactctt ctctgcagaa gtgggttgtt tgcctttggg actggtggaa
    27481 gaatcagggc tgtcctgaat aagcagatcc atggaggaag gaagaggaga gacagtctcc
    27541 gtgtgaggca tctcagcaga agacatttgc aaggatggat agttttcttc aggcccacaa
    27601 atcacaggca taggtgaaga ttctttacag tcggatgctt caaagcaagg caagctttgg
    27661 ggacaagctg gatccacact catggtggct taatcgcgaa ttctttgcca aaatgatgag
    27721 acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat
    27781 ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc
    27841 gtcccccccg aaggcagccg tccccccgcg gacagccccg aggctggaga gggagaaggg
    27901 gacggcggcg cggcgacgca cgaaggccct ccccgcccat ttccttcctg ccggcgccgc
    27961 accgcttcgc cccgcgcccg ctagaggggg tgcggcggcg cctcccagat ttcggctccg
    28021 cacagatttg ggacaaagga agtccctgcg ccctctcgca cgattaccat aaaaggcaat
    28081 ggctgcggct cgccgcgcct cgacagccgc cggcgctccg ggggccgccg cgcccccccc
    28141 ccgagcccyc cccggcccga ggcggccccg ccccgcccgg cacccccacc tgccgccacc
    28201 ccccgcccgg cacggcgagc cccgcgccac gccccgtacg gagccccgca cccgaagccg
    28261 ggccgtgctc agcaactcgg ggaggggggt gcaggggggg ttgcagcccg accgacgcgc
    28321 ccacaccccc tgctcacccc cccacgcaca caccccgcac gcagcctttg ttcccctcgc
    28381 agcccccccc gcaccgcggg gcaccgcccc cggccgcgct cccctcgcgc acactgcgga
    28441 gcgcacaaag ccccgcgccg cgcccgcagc gctcacagcc gccgggcagc gcggagccgc
    28501 acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc cccccgcaca
    28561 aagggccctc ccggagcccc tcaaggcttt cacgcagcca cagaaaagaa acaagccgtc
    28621 attaaaccaa gcgctaatta cagcccggag gagaagggcc gtcccgcccg ctcacctgtg
    28681 ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga ggcggcggcg gagcggggca
    28741 cggggcgaag gcagcgcgca gcgactcccg cccgccgcgc gcttcgcttt ttatagggcc
    28801 gccgccgccg ccgcctcgcc ataaaaggaa actttcggag cgcgccgctc tgattggctg
    28861 ccgccgcacc tctccgcctc gccccgcccc gcccctcgcc ccgccccgcc ccgccyggcg
    28921 cgcgcccccc cccccccccc cccccccccy scmcmaaawa aytaaaaaaa aaataaawac
    28981 aaaaytgggg gkggggrggg gggggagatg gggagagtga agcagaacgt ggggctcacc
    29041 tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
    29101 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
    29161 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
    29221 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
    29281 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
    29341 tatgtaacgc
//
