LOCUS       sequence-326992-       14028 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and 
            donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. 
            Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH
  TITLE     CRISPR RNA-guided integrases for high-efficiency, multiplexed 
            bacterial genome engineering.
  JOURNAL   Nat Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y.
  PUBMED    33230293
REFERENCE   2  (bases 1 to 14028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Nov 23. pii: 10.1038/s41587-020-00745-y. doi: 
            10.1038/s41587-020-00745-y."
COMMENT     Sequence Label: pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
FEATURES             Location/Qualifiers
     source          1..14028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      122..893
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2373..3167
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2427..2446)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3037..3056
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3611..3628
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3639..3673
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             10655..10666
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12101..12119)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12115..12162
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             12314..12973
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(12380..12399)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(13173..13190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13173..13189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13182..13204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(join(13487..14028,1..121))
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg cgttccgttt
       61 gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac tgcgtggcca
      121 tgcttatctc catgcggtag gggtgccgca cggttgcggc accatgcgca atcagctgca
      181 acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca attacagatt
      241 ttctttaacc tacgcaatga gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc
      301 ccttttttaa gttgttgatt tttaagtctt tcgcatttcg ccctatatct agttctttgg
      361 tgcccaaaga agggcacccc tgcggggttc ccccacgcct tcggcgcggc tccccctccg
      421 gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc ggccttcggc cttgcccaag
      481 gtggcgctgc ccccttggaa cccccgcact cgccgccgtg aggctcgggg ggcaggcggg
      541 cgggcttcgc ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
      601 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
      661 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
      721 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
      781 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
      841 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
      901 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
      961 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     1021 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     1081 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     1141 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     1201 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     1261 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     1321 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     1381 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     1441 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     1501 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     1561 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     1621 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     1681 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     1741 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     1801 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     1861 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgtgacctt
     1921 cgggagcgcc tgaagcccgt tctggacgcc ctggggccgt tgaatcggga tatgcaggcc
     1981 aaggccgccg cgatcatcaa ggccgtgggc gaaaagctgc tgacggaaca gcgggaagtc
     2041 cagcgccaga aacaggccca gcgccagcag gaacgcgggc gcgcacattt ccccgaaaag
     2101 tgccacctgg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     2161 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     2221 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2281 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     2341 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2401 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2461 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2521 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     2581 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2641 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2701 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2761 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2821 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2881 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     2941 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3001 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3061 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3121 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3181 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3241 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3301 ccagcgcggg gatctcatgc tggagttctt cgcccacccc catgggcaaa tattatacgc
     3361 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     3421 tccatgtcgg cagaatgctt aatgaattac aacagttttt atgcatgcgc ccaatacgca
     3481 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3541 actggtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3601 gaccgagcgc agcgagtcag tgagcgagga cacaagcttt gacagctagc tcagtcctag
     3661 gtataatact agcgtcgacg tggagatata ccatgggtga actgccgagt aggtagctga
     3721 taacgagacc tctgtcttgt cagctagggt ggtctcgtga actgccgagt aggtagctga
     3781 taacggatcc gaattcgagc gaaggagata tacatatgtt tttgcaaaga cctaaacctt
     3841 acagcgatga aagtttagaa agtttcttta tccgagtggc taacaaaaat ggctacggtg
     3901 atgtccatcg cttcctagaa gccactaaac gattccttca agacattgac cataatggct
     3961 atcaaacctt tccgactgat ataactcgga taaacccata ctcagctaaa aacagttcca
     4021 gcgcacgaac tgcgtcattc ctgaaacttg cacaattgac atttaatgaa ccgccagagc
     4081 tacttgggtt ggcaattaac agaacaaaca tgaaatactc gccgtcaact agcgcggttg
     4141 ttagaggtgc agaagtcttt cctcgcagtt tactacggac gcactccatc ccctgctgtc
     4201 ctttgtgtct gcgagaaaat ggctacgcct cctacctttg gcactttcag gggtacgaat
     4261 actgccacag ccataacgta cctttaatta ccacttgtag ctgtggtaag gagtttgact
     4321 accgagtatc tgggttaaag ggcatttgct gcaaatgcaa ggagcctatc accttaacca
     4381 gcagggagaa cggtcatgag gcagcgtgta ctgtttcaaa ctggcttgct ggccatgaat
     4441 ctaaacctct gccaaatctt cctaaaagct accgatgggg tttagttcat tggtggatgg
     4501 gtattaaaga tagcgagttc gatcactttt cgttcgttca atttttctca aactggccaa
     4561 ggtcattcca ctcgataatc gaagatgaag tagagttcaa ccttgagcat gctgttgtca
     4621 gcacgtctga attacgacta aaagatcttc ttggtcgatt gtttttcggt tcaattcggt
     4681 tacctgagcg gaatcttcaa cacaatatca tccttggtga gcttctctgc tatttagaaa
     4741 atcgtttatg gcaagacaag ggattaatcg ccaacctcaa aatgaacgcg ttagaggcga
     4801 ctgtaatgtt aaattgtagc ctcgatcaga ttgcatcaat ggttgaacaa cgcatcttga
     4861 agccaaatcg aaaaagcaag cccaacagcc ctcttgatgt taccgattat ctatttcatt
     4921 tcggcgatat tttctgtctt tggttagctg agttccaaag cgatgagttt aaccgttcgt
     4981 tttatgtgtc gaggtggtaa atgcaaactc tgaaagaact aatcgcatcc aatcctgacg
     5041 acttaacaac tgaacttaag agagcatttc gtccactcac accgcatatt gcaattgatg
     5101 gtaatgaact tgacgcactg acgatattag tcaatttaac cgataagact gatgatcaga
     5161 aagacctgct cgatcgagcc aaatgcaagc aaaaacttcg agatgaaaaa tggtgggcta
     5221 gctgcataaa ttgcgttaat tacagacaaa gccataaccc aaaattcccg gatatacgtt
     5281 ctgaaggcgt gattcgaacc caagccctgg gtgaattacc gagtttccta ctctcgtcct
     5341 caaaaatccc accataccat tggtcatata gccatgattc aaaatacgtc aacaaaagcg
     5401 cattcctcac caatgagttt tgttgggatg gtgagatctc atgtttgggt gagcttctta
     5461 aagatgcaga tcacccactt tggaatactt tgaaaaagtt aggttgttct caaaaaactt
     5521 gcaaagcaat ggcaaaacaa ctagctgata ttactctcac gactatcaat gtcacactcg
     5581 caccaaatta cctgactcaa atatctctcc ccgatagtga tacatcctac atttcactct
     5641 ctcctgttgc atcgctatcg atgcaaagcc actttcatca gaggctccaa gatgaaaatc
     5701 ggcatagcgc gataacgcgg tttagccgaa ctaccaacat gggggtcact gcgatgacat
     5761 gtggcggcgc atttagaatg ttaaagtctg gcgctaagtt ttccagcccc cctcatcacc
     5821 gattaaacag taaacgaagt tggttgacgt cagagcatgt tcagtcatta aaacagtacc
     5881 agcgcctcaa caaaagcctc atacctgaaa actctcggat tgcactccgt agaaaataca
     5941 aaatcgagct tcaaaatatg gtcagatctt ggtttgcaat gcaagaccat acccttgatt
     6001 cgaacatact tatccaacat ttgaatcatg acctatctta cttaggagcc acaaaacgtt
     6061 ttgcatacga tccagcgatg accaagctct ttactgagct tttgaaacga gagttatcaa
     6121 attcaatcaa taatggtgag caacacacta atggatcgtt tttagtccta ccgaatatca
     6181 gggtttgtgg cgcaacagct ttaagctccc cggtaacggt ggggattcca tcacttacag
     6241 ctttctttgg cttcgttcac gcatttgaac ggaatataaa tcgcaccacc tcatcgtttc
     6301 gtgttgaatc ctttgcgata tgcgtccatc aactacatgt cgagaagcga ggtttgacag
     6361 cagagtttgt ggaaaaaggc gacgggacta tatccgctcc cgcgacccgg gatgactggc
     6421 agtgtgatgt cgtatttagc cttattttga acaccaactt tgctcaacat attgaccaag
     6481 atacgttagt tacatcacta ccaaagcgat tggctcgggg ttcagcaaaa attgcgattg
     6541 atgactttaa acatatcaac tcattctcga cattagaaac agcgatcgaa tctctgccaa
     6601 tagaagctgg taggtggtta tcactttacg cacagtcaaa caataatcta agtgatctat
     6661 tagcagccat gacagaggac catcagctca tggcaagctg cgtcggttac cacttgttag
     6721 aagagcccaa agataaacca aactccctca gaggttacaa acacgctatc gccgagtgca
     6781 tcattggact cattaactca atcaccttta gctcagagac tgatcccaac acaatctttt
     6841 ggtcgctaaa gaactatcaa aactacctag tggtacagcc aaggagtatc aacgatgaaa
     6901 ctaccgacaa atctagccta tgagcgctct atcgacccat cagatgtctg tttttttgtc
     6961 gtctggcccg atgatagaaa aacaccttta acctacaatt ctcgtactct gctcgggcaa
     7021 atggaagcgg catcattagc ctatgatgtc tcaggtcaac caataaaaag tgccaccgct
     7081 gaggcgttag ctcaagggaa ccctcatcaa gttgatttct gccacgttcc atacggtgcg
     7141 agtcatattg aatgcagttt ctccgtctcg ttttcttctg aactacgtca accatataag
     7201 tgtaactcaa gcaaagttaa acaaacgcta gtgcaattag tcgagctcta cgaaacgaaa
     7261 atcggctgga ctgagctagc aacccgatat ttgatgaaca tttgcaacgg taaatggctg
     7321 tggaaaaata cccgtaaagc ctattgctgg aacattgtac ttacaccttg gccttggaac
     7381 ggggaaaagg ttggatttga agatattcgt actaactaca cctcacggca agactttaaa
     7441 aataataaaa attggtctgc tatagttgaa atgatcaaaa ccgcattttc tagtactgat
     7501 gggctggcga tatttgaagt cagggccacc ttgcacttgc caacgaatgc tatggtgcgg
     7561 ccaagccaag ttttcacaga aaaagaaagt ggcagtaaaa gtaaatctaa aactcaaaac
     7621 agtcgagttt ttcagagtac aactattgat ggtgaacgat cgccaatact aggggccttt
     7681 aaaacgggag cagctattgc aaccattgac gactggtatc ctgaagccac tgagccacta
     7741 agggtcggac ggtttggggt tcatcgcgaa gatgtcactt gctaccgtca tccgtctacc
     7801 ggaaaagatt ttttctcgat attacaacaa gcagagcact atattgaagt gttgagcgcc
     7861 aacaaaactc ccgctcaaga aactatcaac gacatgcact ttttaatggc taacctgatt
     7921 aagggtggga tgttccagca taaaggagac tgactgtgaa atggtattat aagacaatca
     7981 cctttctgcc agagttgtgc aacaacgagt cactggctgc aaagtgtctc cgcgttctgc
     8041 atggatttaa ctatcagtat gagacacgaa atatcggcgt ttcatttccg ctttggtgtg
     8101 atgcaacggt tggaaaaaag atttcatttg tcagcaagaa caagatagaa ctcgacttac
     8161 tacttaaaca acactatttc gtccaaatgg aacaacttca atactttcat atatccaaca
     8221 ctgttctcgt cccagaagat tgtacatacg tttcctttag acgctgtcaa tctatagata
     8281 agctcacagc agcagggctg gcaaggaaaa tcagacgcct ggagaaacgt gctctatcta
     8341 gaggcgagca atttgaccca tcatcttttg ctcaaaaaga gcatactgca atagcgcact
     8401 accactcact tggggagtcc agcaaacaga cgaaccgcaa ctttcgactc aatatcagga
     8461 tgctctcgga gcaaccccgt gaggggaact cgatttttag tagctatggc ttatcaaatt
     8521 cagaaaactc gtttcagcct gtacccttaa tctgaccttt aataaggaga tataccatgg
     8581 cgacaagttt acctacgccc tcagcaatta cgacttcggc gttagagtat gcattccata
     8641 ctcccgctcg caatctaacg aaatctcgcg gaaaaaacat tcatcgttat gtcagtgtaa
     8701 agatgagtaa gaggattacg gtagaatcta ctctagagtg tgatgcctgc tatcactttg
     8761 attttgagcc aagtattgtt cgcttttgcg ctcaaccgat tcgattttta tattatctca
     8821 atggtcagtc tcactcctat gttcctgact ttctagttca atttgatacc aacgagtttg
     8881 ttctatatga agtaaagtca gcttatgcta agaacaaacc tgattttgat gttgaatggg
     8941 aggcgaaagt aaaagcagca actgaactag ggctagaatt ggagcttgtt gaagagagtg
     9001 atattaggga tacggttgta ttaaataatc ttaagcgcat gcatcgttat gcttcgaaag
     9061 atgagctgaa taacgtacat aactctctct taaaaataat aaagtacaat ggcgcccaat
     9121 ctgcaagatg cttgggagaa cagttgggtt taaaaggccg aactgtttta ccaattttgt
     9181 gcgatttgct gtcaaggtgt ttactcgata cacgtttgga taagcctcta tctcttgaat
     9241 ctcgatttga gttggccagt tatggctaag aaagggttct caagtttcca tagaaaagca
     9301 gtctcttccc aagatacgct tgaatcgata gagcttgtct ctagcgctaa ttgtctagaa
     9361 agtgttacgt atcaagatat atcagcattt cccgaaacaa ttgcggtaga gattaatttc
     9421 cgattaagca ttcttcgctt tttagcgcga aagtgcgaaa ccattgtggc caaatcaatt
     9481 gaaccacatc gtgtagagct acagcaaaac tatagtagaa aaatacccag tgcaataacg
     9541 atatatcgat ggtggcttgc ttttcgaaaa tcagactaca accccattag cttagcacct
     9601 aatatcaagg atagaggtaa tagagagaca aaagtgtcaa cagttgttga ttctattatg
     9661 gaacaggcag ttgaaagagt tatatctgga cgaaaagtca atgttagctc tgcatataaa
     9721 cgtgttcgac gaaaagttcg tcaatacaat ctaactcatg gaacgaaata cacgtatcct
     9781 aagtacgaat ctgtaagaaa gcgagtaaaa aagaaaaccc catttgagtt attagccgca
     9841 gggaaagggg agagagtagc taagagagag tttcgccgaa tgggaaaaaa gatcctcacg
     9901 tctagcgtgc tagagagggt tgaaatagat cacactgtcg ttgacctttt tgcagtacac
     9961 gaagagtatc gaatcccatt gggccgacct tggcttactc aattggttga ttgttacagt
    10021 aaagctgtaa tcggttttta tttaggtttc gagcctccta gctatgtgtc ggtttccctt
    10081 gcacttaaga atgcaataca acgcaaagat gacttaatct cctcgtatga atcgatcgag
    10141 aatgaatggc tatgttatgg catcccagac ctactcgtaa ctgataatgg taaagagttt
    10201 ttgtcgaaag catttgatca agcatgtgaa tcactattga tcaatgtgca tcaaaataaa
    10261 gttgagacgc ccgacaacaa acctcatgtt gaacgtaact acgggactat taatacttct
    10321 ctgttagacg atttacctgg gaaatccttc agccagtacc ttcaaagaga agggtacgac
    10381 tctgtgggag aagctaccct tacactcaat gagattagag aaatttactt aatttggttg
    10441 gtggatattt atcataaaaa acccaatcag agaggcacta attgtcctaa tgttgcgtgg
    10501 aaaaagggtt gtcaagaatg ggaaccagag gagttctctg gttctaaaga cgaattagac
    10561 tttaaatttg ctattgttga ttacaaacaa cttactaaag tagggataac tgtctacaaa
    10621 gaactgagtt atagcaatga ccgtttagct gaatatagag ggaagaaagg aaaccataaa
    10681 gttcagttca agtataaccc tgagtgtatg gcagttattt gggtgttgga tgaggatatg
    10741 aatgagtact ttacagttaa tgcgattgac tacgaatatg caagtagagt atcactttgg
    10801 caacataaat ataacatgaa atatcaagca gaactaaatt cagcagaata tgatgaggac
    10861 aaggaaattg atgcagaaat aaaaattgaa gaaatcgcag atcgttcaat tgttaagact
    10921 aacaaaatca gagctcggag gcgtggcgct aggcatcaag agaatagcgc aagggctaag
    10981 tcaatcagta atgcgaaccc ggcctcgata caaaaacatg aagatgaaat cgttagtgca
    11041 gataatgacg attgggatat tgattatgtc tgagaaatcg tcaaatgagt gaaacgcgtg
    11101 aggctcgtat atcaagagct aaaagggcat ttgtatccac accgagcgtt aggaaaatct
    11161 tgagttacat ggatagatgt agagatctat cagacctaga gtctgagcct acatgcatga
    11221 tggtctatgg tgcttcgggt gtaggtaaaa cgaccgtcat caagaaatac ttaaatcaaa
    11281 acagaagaga gtccgaagcc gggggcgata taataccggt tttgcatatt gagttgccag
    11341 acaatgcgaa gccagtagat gcagcaaggg aattgctggt tgaaatgggt gacccgctag
    11401 cactttatga aactgactta gctagattga cgaaaagact gactgaatta atccctgcgg
    11461 tcggcgtgaa gctgattatt atcgatgagt tccaacattt ggtggaagaa aggtcaaatc
    11521 gggttcttac ccaagtaggt aattggctaa aaatgatact taacaaaacg aaatgtccaa
    11581 ttgttatatt tggtatgcca tactcaaaag ttgtactgca agcaaactcg caacttcacg
    11641 ggcgattttc cattcaggtt gaactgcgcc cctttagcta ccagggaggt agaggtgtat
    11701 ttaaaacttt tttggaatac cttgataaag ccctaccttt tgaaaaacag gctggcttag
    11761 ccaacgaaag tttgcagaaa aaattgtatg cattctctca gggaaacatg cgttcgttga
    11821 gaaaccttat ttatcaagca tctatcgaag caattgataa tcagcatgag acgataaccg
    11881 aagaagattt cgtttttgca tcgaagttga catcgggcga taaacccaac tcatggaaaa
    11941 atccttttga ggagggtgtt gaggtaacag aagatatgtt acgaccgcca ccaaaagata
    12001 ttggttggga ggactatttg agacattcaa ccccgagagt gagtaaacca ggtagaaata
    12061 aaaacttttt cgaataacct aggctgctgc gctgctgcca ccgctgagca ataactagca
    12121 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcatgt
    12181 tggaacaacc ataaaatgat aattacaccc ataaattgat aattatcaca cccataaatt
    12241 gatattgcct cttcatggtc taaacttcag taagtttacg acattttcat gaagaggtca
    12301 tttccgggga tccatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
    12361 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
    12421 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
    12481 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggagt tccgtatggc
    12541 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
    12601 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
    12661 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
    12721 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
    12781 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacta tgggcaaata
    12841 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
    12901 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
    12961 gggcggggcg taattttttt aaggcagtta ttggtgccct tctagagtcc ttactgcagt
    13021 agttttgctg aaatactcga ttcacaaaaa tatcaactta tggttgtttt gtgagatatc
    13081 aatatatggt tgttttgtgg ttaagttgct gattataaat aattattaaa tatcacttta
    13141 tggttgcatc aacaggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
    13201 tgggaaaacc ctggcgttac ccaacttaat cgccttgcgg ccagtttgct caggctctcc
    13261 ccgtggaggt aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa
    13321 cggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca
    13381 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc
    13441 gatagtctgg aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca
    13501 gcgtgacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg
    13561 gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca
    13621 ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg
    13681 gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca
    13741 gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca
    13801 actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc
    13861 cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt
    13921 attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca
    13981 cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattg
//
