LOCUS       sequence-327048-        4896 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Zebrafish kcnn3 gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnn3
FEATURES             Location/Qualifiers
     source          1..4896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(184..283)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(299..316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(299..315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      379..465
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      557..584
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(743..760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(914..1502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(994..1013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1649..2458)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2372..2391
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2450..2469
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2571..2587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2571..2587
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2592..2611
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2592..2610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2628..2727
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctccaggct gctgttccct cccgtgtgat tcgagtggtt ggtcccaggg tagttgccag
       61 agagggaggc atgggaaata ccaccgagag ggaggggcag acgctgctct tcgtcttcac
      121 tcccgctgag aagggccaga tttagagagg gagggggatc catggtgaag ggggcggccg
      181 cggagcctgc ttttttgtac aaagttggca ttataaaaaa gcattgctca tcaatttgtt
      241 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggggcccg agcttaagac
      301 tggccgtcgt tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag
      361 tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt
      421 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
      481 ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
      541 aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
      601 cttcctggct agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg
      661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca
     1561 cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgcc
     1621 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1681 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1741 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1801 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1861 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     1921 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     1981 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     2041 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     2101 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg
     2161 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     2221 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     2281 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaagc
     2341 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2401 cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa
     2461 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     2521 tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc caggaaacag
     2581 ctatgaccat gtaatacgac tcactatagg ggatatcagc tggatggcaa ataatgattt
     2641 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa
     2701 tgccaacttt gtacaagaaa gctgggtcgg cgcgcccacc ctttcaacaa ctggagctgc
     2761 tggtgttggg actgtcctct gcgggctgag ttgaggggat ggagcctgag ccggaggggg
     2821 ggttggctag aggcaggctg gcagcagtgg gtgagagcgc ggagggcaca gtggggctca
     2881 gaaccttggc atcacgctca cgcagcagtt gaacgagcgc cgcatgctgg gagctgagcg
     2941 tggccgacaa gtgagcgggc agagcattaa agccggccgt caaacgctcc acccgctgct
     3001 ccagactcaa catctgcctc tccagatcct cacttcggtc gttcagctct gacatcagtt
     3061 catacatgac gctctgcatc tttgataagt ccaccaatgt attggcttga tcactgagtt
     3121 ttctctgctc catttttacg cttcgcaatt ggtgaatggc ttgaaggaac ttcctctgat
     3181 gtttgcgcac acgggagtgg tcgattttct tcatcagctt ggtgtgcttg taaatcagcc
     3241 aagtctctct gagaacattc gctgctgcgt tctttatccg tttggtgagc tgtgtgtcca
     3301 tcatgaagtt atgcacatgt ttctctgcct tcgtcagctc cagctttctg gcaaccacag
     3361 caacaaccaa tgcagtgcaa cctgctccca taattcctgt gagcagacac actcccttgc
     3421 cacagtaggt gtgaggcacc atgtctccat atccaataga gaggaaggtg atagagatga
     3481 gccacattgc acccaaaaaa ttactggtca catcctgagc gtcatggtac ctctcacaca
     3541 ctcgtacggt ccaggcagcg atgatccaca gggagatact gaagactagc agcaccgtgc
     3601 ccgggcatat ggtcatcagg gtcttcatca caaagcgtgt gttaaagtgc accttgttga
     3661 gtgcccctat gctgcgggag gaggcgtccg tgaagagctt gctatgtagc agcatgacgc
     3721 gggcgatgag gtagagtcta agaaacatgg gtacagacag caccatgtct agatctgcct
     3781 ccgcctgcga gggagcatag gagaaggcca gtctcgcccg ccacataaac ttgaagtccc
     3841 cgggcaccgg gtgaaccgcc gaaacgatca gctccagagc gataagcagc accctctcca
     3901 tggtcatggc aattcgccag tcgtctgcac cattgtcgat gacgaaaagc tgcacctctc
     3961 gagcatggta agcgatgatt agtccaagaa ggatgatggt ggaaaggcta ataagacact
     4021 tcaatgccag agaatacatg gagctcttac tgtagacacc ccaggacagc tcggtctcaa
     4081 tcaccatgac aacaatgcca aacatgccga atatgagggc atagtcacta agtctcttcc
     4141 tcttttcgaa cagtgccctc ctgtggccta gtttgtagcc aatgttctga ttcttacgtt
     4201 taggagcttt agaagcggtc cttccaggcc ctcccgcagc accgcccctc cttccattag
     4261 tcccgccccc tgccgcccca ccgagcacac tcctgctctc agagagggcg tggttctggt
     4321 ggtagatgga catttgattg gctgaggcct caagttcgta agcatgtcca taaggcggat
     4381 cctctttcga ggagataacg atctctgggg ggttctgggg ttggggctgg ctgtgctgag
     4441 aggaggccgc ggtcacctgg cttgtgctcg caccaccctc tttggtgtca cttccgctgt
     4501 cggactcgat gagattccgg cgggaggcgc taaggcggct aagtggcttc atcacaccac
     4561 cgctgtactt gcacgagctc atggcgatct ctgtaaaagg attactgtcc cgcctgtgca
     4621 ccaggggact ggcctgcctg tgcttacatc ccctctctcg ctcccgttcc cgctcagtgg
     4681 aaggtgaatg agaagagtaa aacagagcat tgtagacggg tgaagtctcc ccgcttaagc
     4741 tgtgttggct tcccaagcag gaggagagtg gcgtactggt cgggtgaaga gcgttgggga
     4801 ttggtgtgga aagctggggt gttgtggaca ggggcaactt gggcagtgac gctgggggaa
     4861 ggtttggcgt ggtgggcgat ggagtgccat tgagac
//
