LOCUS       sequence-327575-        7324 bp    DNA     circular SYN 14-APR-2025
DEFINITION  rig-3 promoter derive BFP expression in AVA head neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Hallinen KM, Dempsey R, Scholz M, Yu X, Linder A, Randi F, Sharma 
            AK, Shaevitz JW, Leifer AM
  TITLE     Decoding locomotion from population neural activity in moving C. 
            elegans.
  JOURNAL   Elife. 2021 Jul 29;10:e66135. doi: 10.7554/eLife.66135.
  PUBMED    34323218
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-07-29"; volume: "10:e66135. doi"; pages: " 
            10.7554/eLife.66135"
COMMENT     Sequence Label: pAS1-Prig-3::tagBFP
FEATURES             Location/Qualifiers
     source          1..7324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..29
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     243..265
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     257..274
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     258..274
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     284..303
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        284..302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4794..4814)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4794..4812)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4833..4849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4833..4849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4846..4868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4857..4873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4881..4887,4888..4905,4906..4911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4926..4947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5064..5081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5235..5823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5315..5334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5994..6785,6786..6854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6617..6636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6855..6959)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(6985..7324,1..116))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7122..7143)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
       61 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
      121 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      181 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      241 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
      301 gggcgaattg gagctccacc gcggactagt ggatcccccg ggctgcagaa tgcgacccat
      361 catattgttc ctattgtcat gtacaagctg tccctgtttt gctcgcttaa tccgtcaacc
      421 ccttccccgt tctaattaga gatgagtgaa ttacaataga gttgaaacgg aaatctgagt
      481 gtctgtagct ttccgccgcc gcttttgtta cgagatggta gacgtccctt tcttggtgtt
      541 tattgtctca caaactgtat aataaagcac cgttgtgtaa aaggcatttg gtatcaggaa
      601 gataaaatga ataaaatgca aatgcggaat caaaaattgc caaataattt tgaccaataa
      661 aatatcaaaa ataatagaac agaaataaac aaataaaact acggagaaaa tgtgattatt
      721 ccattaaaca acgttttaaa ccaactttat tcgtcagtgg ggaaggtttt ggacaaatac
      781 catagtaaaa cttgttcgtt ttaattatac ataatcaata attataggtg ttagaaccaa
      841 tggtcgaaat ttaaaaaaaa atcaacattt tagaaaattt cggcaattta ccgaaatatt
      901 gactgacaat tttacatata taagtgtttt gaaatagaat ccccattgac gatgcgccac
      961 cttaaacagt agaactgttc caatcagaaa ctaaataaat acggttatta tatatatata
     1021 tatatatata tatatataac ttgtttttat tatgcatttc tcaaaactca ccaaactcaa
     1081 tctctaattt acggttgttg aggaaaatat atttatcaca tttagttgag actctaaaag
     1141 aagtacaact tttttcttgc aattcctcca ccaatccaat ttttcaagag tcaattgctt
     1201 ggagtcctcg ttttctcgta atccgatatt ccccctattt ctgtgatttt attcagtttc
     1261 gtcctcttac accaaaaatc ttcatgactt ttttggtttt ctgcttaatc ttttttttgt
     1321 ttttaaagtc taccatccgg caattttttt tgcaaatggt tgctcagaat ttgacctcag
     1381 attaaaattt tcaggaactt cctggtccca tttcgtcaca tttaataaca aaccaaatcc
     1441 ttcagcttga gtcgtccaag ccaacgcgac cctaattcga ttccacctcc aatttacctt
     1501 tttgccgttt tcgatcaaat tgtgggggct ctttttattt gaaagtcgta aaatggcgcg
     1561 tccttgtcga aaattgagat agctaatggt tccaaataaa atcaaatgca tgaacctagt
     1621 aggaaaaatc gttttttttt tcatcttcca aagaaaaagt tcaagataaa cccaccactc
     1681 cagcaatcca cctacgcgtt tttcaccgga aactgtcatt tgtttttgat gtatgacagt
     1741 gccagcactg aactaaattc ccatttgttt tgttgtggtg ttatctaacc acgtggctct
     1801 ttctctaaat tttgcgtagg ctgcaagatc cgcaatccca ccaaaaaatg gtttctaatg
     1861 tacttcatac atatcccaca tgactcagtg ctccgaaacg cccagcaacc cccgcgtttg
     1921 tctttacaaa aatgaggcgg aaatgggaaa ttttcctgct attgctatgg taaaatactt
     1981 aagctgtatt gagatttggg aaattgaaag ataagatgaa aaatccactc tgtataatta
     2041 aaaaggttta ttttttttgt aatttttgta ccgcaaattt tttcattaac tgatttttct
     2101 ttattcattc gatggcccag tttcagatct tcattatcat gtagtttatt cattcaaact
     2161 atttgtgaca tcatgatggt tgtttaaaaa taatttcgaa tgaagaaagc ttaaacctat
     2221 tataattttc ttttctataa aaaatattga ttttgtaaag cgtttggcag tgacagtacc
     2281 aaaaaatgtt ttaaaatgat ttaagaaaat tttttatttt atttgtataa tacgcgtaaa
     2341 atgagtatac tcgtcatttg tcacgaatcg agttctcagt ttgctgtgaa tatcttgtaa
     2401 tagaaatcga tctcatagtg tcacttttga ggcataactt ttcaggttgt tacaagttca
     2461 ttaacttgtc aagactgggt catgttttaa agtgtaactg ttacccatgt accctaaact
     2521 ttgagacaaa ctcctcaaaa cttgtaacca gaatcctatg tagatgactc acaaaaccct
     2581 gttttcaatt tgaacgaatt tcgaaactgg ccaaaatgtt cccctcttgc tcacctaatg
     2641 cttttaccat ttaaaaagga tggtaacaac actctttcga aaatgtaggc ggtccatttt
     2701 cggtttctca taaccgtctg gctctatctc ctagtgcgcg aggagagggc gggcggagta
     2761 ggaatcgggg gcagcgcact catacactct gcccttctct tttgcacaca cagaaaaaga
     2821 tagatacaga ccaacactta ttgttgtcca gtaagcttcc agaaaactat tttcagaatt
     2881 acaaactaca tttttctaga acagtcaatt gattttttac aagattcgag tattctaaca
     2941 ttagtgaaag tatttagcca ctgcaatttt caactgtagg tttcaacttc caaaaaaaat
     3001 tcctagcatt tctaattgga tgaaaaaagg ctatctagac cctccattat ttactcataa
     3061 tttcatctcg tcctcatttt taatcccctc attgatccca tcacaatccg ccaaaatgtt
     3121 ttttaaacac ttttttcttc accagccgta tgtggtcaac tgcctaaaaa tttcacagct
     3181 atttgtcatt ccttgcagaa gaacttcatt cttctttttc gaaaatgtcc gaactcatca
     3241 aggagaacat gcacatgaag ctctacatgg agggaaccgt agacaaccac cacttcaagt
     3301 gcacctccga gggagaggga aagccatacg agggaaccca aaccatgcgt atcaaggtcg
     3361 tcgagggagg accactccca ttcgccttcg acatcctcgc cacctccttc ctctacgggt
     3421 ccaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagacct
     3481 tcatcaacca cacccaagga atcccagact tcttcaagca atccttccca gagggattca
     3541 cctgggagcg tgtcaccacc tacgaggacg gaggagtcct caccgccacc caagacacct
     3601 ccctccaaga cggatgcctc atctacaacg tcaagatccg tggagtcaac ttcacctcca
     3661 acggaccagt catgcaaaag aagaccctcg gatgggagac cttcaccgag accctctacc
     3721 cagccgacgg aggactagag ggacgtaacg acatggccct caagctcgtc ggaggctccc
     3781 acctcatcgc caacatcaag gtaagtttaa acagttcggt actaactaac catacatatt
     3841 taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag atgccaggag
     3901 tctactacgt tgactaccgt ctagagcgta tcaaggaggc caacaacgag acctacgtcg
     3961 agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc ggacacaagc
     4021 tcaactaaaa gctttggaat tcatctcgcg cccgtgcctc tgacttctaa gtccaattac
     4081 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     4141 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     4201 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     4261 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     4321 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     4381 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     4441 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     4501 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc
     4561 tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     4621 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     4681 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     4741 cattcccaat ataccaaaca taactgtttg ggcccggtac ccagcttttg ttccctttag
     4801 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4861 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
     4921 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4981 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5041 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5101 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5161 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5221 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5281 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5341 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5401 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5461 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5521 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5581 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5641 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5701 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5761 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5821 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5881 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5941 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6001 tgcttaatca gtgaggcacc tatctcagcg atctgcctat ttcgttcatc catagttgcc
     6061 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6121 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6181 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6241 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6301 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6361 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6421 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6481 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6541 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6601 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6661 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6721 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6781 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6841 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6901 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6961 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     7021 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7081 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7141 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7201 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7261 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     7321 tggc
//
