LOCUS       sequence-327813-        7085 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Human DDX41 mammalian expression vector with C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME
  TITLE     TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 
            10.1073/pnas.1322545111. Epub 2014 Apr 28.
  PUBMED    24778252
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1322545111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-13- 13"; volume: "111"; issue: "19"; pages: "6970-5"
COMMENT     Sequence Label: pCMV3Tag8li-hDDX41-HA
FEATURES             Location/Qualifiers
     source          1..7085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2476..2502
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2527..2598
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(2651..2670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2652..2670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2699..2716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2705..2912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2919..3374)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3056..3077)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3268..3287
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3401..3505
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3506..3574,3575..4363)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3724..3743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        4457..4602
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             4645..5682
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(5680..5699)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5724..5772
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6323..6911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6812..6831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggctcga cgccatggag
      601 gagtcggaac ccgaacggaa gcgggctcgc accgacgagg tgcctgccgg aggaagccgc
      661 tccgaggcgg aagatgagga cgacgaggac tacgtgccct atgtgccgtt acggcagcgc
      721 cggcagctac tgctccagaa gctgctgcag cgaagacgca agggagctgc ggaggaagag
      781 cagcaggaca gcggtagtga accccgggga gatgaggacg acatcccgct aggccctcag
      841 tccaacgtca gcctcctgga tcagcaccag caccttaaag agaaggctga agcgcgcaaa
      901 gagtctgcca aggagaagca gctgaaggaa gaagagaaga tcctggagag tgttgccgag
      961 ggccgagcat tgatgtcagt gaaggagatg gctaagggca ttacgtatga tgaccccatc
     1021 aaaaccagct ggactccacc ccgttatgtt ctgagcatgt ctgaagagcg acatgagcgc
     1081 gtgcggaaga aataccacat cctggtggag ggagacggta tcccaccacc catcaagagc
     1141 ttcaaggaaa tgaagtttcc tgcagccatc ctgagaggcc tgaagaagaa aggcattcac
     1201 cacccaacac ccattcagat ccagggcatc cccaccattc tatctggccg tgacatgata
     1261 ggcatcgctt tcacgggttc aggcaagaca ctggtgttca cgttgcccgt catcatgttc
     1321 tgcctggaac aagagaagag gttacccttc tcaaagcgcg aggggcccta tggactcatc
     1381 atctgcccct cgcgggagct ggcccggcag acccatggca tcctggagta ctactgccgc
     1441 ctgctgcagg aggacagctc accactcctg cgctgcgccc tctgcattgg gggcatgtcc
     1501 gtgaaagagc agatggagac catccgacac ggtgtacaca tgatggtggc caccccgggg
     1561 cgcctcatgg atttgctgca gaagaagatg gtcagcctag acatctgtcg ctacctggcc
     1621 ctggacgagg ctgaccgcat gatcgacatg ggcttcgagg gtgacatccg taccatcttc
     1681 tcctacttca agggccagcg acagaccctg ctcttcagtg ccaccatgcc gaagaagatt
     1741 cagaactttg ctaagagtgc ccttgtaaag cctgtgacca tcaatgtggg gcgcgctggg
     1801 gctgccagcc tggatgtcat ccaggaggta gaatatgtga aggaggaggc caagatggtg
     1861 tacctgctcg agtgcctgca gaagacaccc ccgcctgtac tcatctttgc agagaagaag
     1921 gcagacgtgg acgccatcca cgagtacctg ctgctcaagg gggttgaggc cgtagccatc
     1981 catgggggca aagaccagga ggaacggact aaggccatcg aggcattccg ggagggcaag
     2041 aaggatgtcc tagtagccac agacgttgcc tccaagggcc tggacttccc tgccatccag
     2101 cacgtcatca attatgacat gccagaggag attgagaact atgtacaccg gattggccgc
     2161 accgggcgct cgggaaacac aggcatcgcc actaccttca tcaacaaagc gtgtgatgag
     2221 tcagtgctga tggacctcaa agcgctgctg ctagaagcca agcagaaggt gccgcccgtg
     2281 ctgcaggtgc tgcattgcgg ggatgagtcc atgctggaca ttggaggaga gcgcggctgt
     2341 gccttctgcg ggggcctggg tcatcggatc actgactgcc ccaaactcga ggctatgcag
     2401 accaagcagg tcagcaacat cggtcgcaag gactacctgg cccacagctc catggacttc
     2461 gcggccgcaa tcgattaccc atacgatgtt ccagattacg ctctttagga attcttcgat
     2521 gtcgaggatt acaaggatga cgacgataag gactataagg acgatgatga caaggactac
     2581 aaagatgatg acgataaata gggcccggta ccttaattaa ttaaggtacc aggtaagtgt
     2641 acccaattcg ccctatagtg agtcgtatta caattcactc gatcggctcg ctgatcagcc
     2701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2881 gattgggaag acaatagcag gcatgctggg gaacgcgtaa attgtaagcg ttaatatttt
     2941 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3001 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3061 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3121 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3181 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3241 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3301 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3361 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3481 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3601 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3661 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3781 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3841 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3901 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3961 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4021 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4081 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4141 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4201 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4261 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4321 tagacagatc gctgagatag gtgcctcact gattaagcat tggcctcagg attagtgctt
     4381 catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca
     4441 tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa
     4501 cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg
     4561 tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc
     4621 gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     4681 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     4741 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     4801 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     4861 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     4921 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     4981 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     5041 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     5101 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     5161 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     5221 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     5281 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     5341 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     5401 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     5461 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     5521 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     5581 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     5641 cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat
     5701 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     5761 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     5821 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     5881 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca
     5941 ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt
     6001 tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg
     6061 gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct
     6121 gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg cgccgccgga
     6181 cgaactaaac ctgactacga catctctgcc ccttcttcgc tggtacgagg agcgctttgc
     6241 ctcaggtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     7021 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7081 ttccg
//
