LOCUS       sequence-327837-        8536 bp    DNA     circular SYN 21-MAY-2025
DEFINITION  Lentiviral vector that expresses an EGFP-Cre fusion protein with a 
            nuclear localization signal from the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Truitt JM, Blednov YA, Benavidez JM, Black M, Ponomareva O, Law J, 
            Merriman M, Horani S, Jameson K, Lasek AW, Harris RA, Mayfield RD
  TITLE     Inhibition of IKKbeta Reduces Ethanol Consumption in C57BL/6J Mice.
  JOURNAL   eNeuro. 2016 Oct 31;3(5). pii: ENEURO.0256-16.2016. eCollection 2016
            Sep-Oct.
  PUBMED    27822501
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eNeuro."; 
            date: "2016-10-31"; volume: "3(5). pii"; pages: " 
            ENEURO.0256-16.2016. eCollection 2016 Sep-Oct"
COMMENT     Sequence Label: pLV-EGFP-Cre
FEATURES             Location/Qualifiers
     source          1..8536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             608..628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(629..631,632..1342)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(629..631,632..1342)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(932..951)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1279..1300
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1364..2395
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    2461..3049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2514..2534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2932..2943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3136..3369
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3441..3562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3478..3497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3532..3551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3581..3716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3736..3755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3737..3755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3765..3782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3765..3781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3774..3796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3923..4378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4010..4029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4220..4241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4404..4508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4509..4577,4578..5369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4727..4746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5540..6128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6029..6048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6282..6299
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6416..6437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6452..6457,6458..6475,6476..6482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6490..6506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6495..6517
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6514..6530
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6514..6530
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6549..6569
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6551..6569
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6597..6823
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6824..7004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7051..7176
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7669..7902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8087..8131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8398..8514
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc taccggtcgc
      601 caccatgccc aagaagaaga ggaaggtggt gagcaagggc gaggagctgt tcaccggggt
      661 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      721 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      781 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      841 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      901 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      961 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1021 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1081 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1141 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1201 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1261 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1321 cggcatggac gagctgtaca agtccggccg gactcagatc tccatgtcca atttactgac
     1381 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     1441 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     1501 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     1561 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     1621 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     1681 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
     1741 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     1801 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     1861 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     1921 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     1981 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     2041 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     2101 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     2161 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     2221 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     2281 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     2341 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg attaggaatt
     2401 ctgcagtcga cggtaccgcg ggcccgatcc accgggtaca agtaaagcgg ccgcgtcgac
     2461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2821 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     2881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     3061 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3121 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3181 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3361 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3421 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3541 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3721 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     3781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3901 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4321 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6181 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6241 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6301 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6361 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6421 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6481 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6541 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     6601 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     6661 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     6721 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     6781 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     6841 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6901 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6961 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7021 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7081 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7141 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7201 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7261 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7321 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7381 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7441 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7501 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     7561 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     7621 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     7681 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     7741 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     7801 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     7861 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     7921 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     7981 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8041 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8101 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8161 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8221 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8281 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     8341 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     8401 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     8461 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat
     8521 aagcttggga gttccg
//
