LOCUS       sequence-328029-       10622 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway destination clone of CAMK1D (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10622)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CAMK1D_IDG-K
FEATURES             Location/Qualifiers
     source          1..10622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    139..255
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    289..309
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        309..1520
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1357..1376
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1524..1544)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1545..1554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1557..2327
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2334..2375
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2376..2400
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3565..3589)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3619..4207
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3672..3692)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4090..4101)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4221..4720
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4256..4279)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4653..4672
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4741..5340
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4741..4760)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5237..5257
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5478..5711
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5783..5904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5820..5839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5874..5893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5944..6079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6099..6118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6100..6118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6128..6145)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6128..6144)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6137..6159)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6286..6741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6373..6392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6583..6604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6767..6871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6872..6940,6941..7732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7903..8491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8392..8411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8645..8662
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8779..8800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8815..8820,8821..8838,8839..8845)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8853..8869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8858..8880
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8877..8893
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8877..8893
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8912..8932
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8914..8932
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8960..9186
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9187..9367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9414..9539
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10032..10265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10450..10494
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
       61 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
      121 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      181 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      241 aaaattcaaa attttatcgt ccagtgtggt ggaattctgc agatatcaca actttgtata
      301 gaaaagttgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
      361 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
      421 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
      481 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
      541 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
      601 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      661 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
      721 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
      781 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
      841 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
      901 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
      961 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     1021 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     1081 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     1141 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     1201 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     1261 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     1321 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     1381 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     1441 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     1501 ttttggcttt tttgttagac ggccaacttt tgtatacaaa gtgggccacc atgggaatcc
     1561 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
     1621 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
     1681 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
     1741 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
     1801 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
     1861 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     1921 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
     1981 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
     2041 tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg atccgagagc
     2101 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg
     2161 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
     2221 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
     2281 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggct agcggcaagc
     2341 ccatccccaa ccccctgctg ggcctggaca gcaccacaag tttgtacaaa aaagcaggct
     2401 ccaccatggc ccgggagaac ggcgagagca gctcctcctg gaaaaagcaa gctgaagaca
     2461 tcaagaagat cttcgagttc aaagagaccc tcggaaccgg ggccttttcc gaagtggttt
     2521 tagctgaaga gaaggcaact ggcaagctct ttgctgtgaa gtgtatccct aagaaggcgc
     2581 tgaagggcaa ggaaagcagc atagagaatg agatagccgt cctgagaaag attaagcatg
     2641 aaaatattgt tgccctggaa gacatttatg aaagcccaaa tcacctgtac ttggtcatgc
     2701 agctggtgtc cggtggagag ctgtttgacc ggatagtgga gaaggggttt tatacagaga
     2761 aggatgccag cactctgatc cgccaagtct tggacgccgt gtactatctc cacagaatgg
     2821 gcatcgtcca cagagacctc aagcccgaaa atctcttgta ctacagtcaa gatgaggagt
     2881 ccaaaataat gatcagtgac tttggattgt caaaaatgga gggcaaagga gatgtgatgt
     2941 ccactgcctg tggaactcca ggctatgtcg ctcctgaagt cctcgcccag aaaccttaca
     3001 gcaaagccgt tgactgctgg tccatcggag tgattgccta catcttgctc tgcggctacc
     3061 ctccttttta tgatgaaaat gactccaagc tctttgagca gatcctcaag gcggaatatg
     3121 agtttgactc tccctactgg gatgacatct ccgactctgc aaaagacttc attcggaacc
     3181 tgatggagaa ggacccgaat aaaagataca cgtgtgagca ggcagctcgg cacccatgga
     3241 tcgctggtga cacagccctc aacaaaaaca tccacgagtc cgtcagcgcc cagatccgga
     3301 aaaactttgc caagagcaaa tggagacaag catttaatgc cacggccgtc gtgagacata
     3361 tgagaaaact acacctcggc agcagcctgg acagttcaaa tgcaagtgtt tcgagcagcc
     3421 tcagtttggc cagccaaaaa gactgtctgg caccttccac gctctgtagt ttcatttctt
     3481 cttcgtcggg ggtctcagga gttggagccg agcggagacc caggcccacc actgtgacgg
     3541 cagtgcactc tggaagcaag taggacccag ctttcttgta caaagtggtg atatccagca
     3601 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3661 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3721 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3781 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3841 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3901 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3961 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4021 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4081 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4141 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4201 tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     4261 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     4321 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     4381 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     4441 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     4501 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     4561 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     4621 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     4681 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     4741 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4801 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4861 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4921 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4981 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5041 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5101 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5161 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5221 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5281 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5341 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga
     5401 gggcccggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5461 aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt
     5521 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5581 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5641 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5701 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     5761 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     5821 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5881 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     5941 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6001 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6061 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     6121 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6181 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6241 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     6301 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6361 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6421 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6481 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6541 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6601 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6661 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6721 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6781 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6841 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6901 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6961 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7021 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7081 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7141 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7201 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7261 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7321 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7381 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7441 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7501 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7561 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7621 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7681 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7741 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7801 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8041 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8581 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8641 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     8701 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8761 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8821 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     8881 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     8941 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     9001 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     9061 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     9121 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     9181 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9241 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9301 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9361 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9421 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9481 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     9541 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     9601 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9661 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9721 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9781 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9841 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     9901 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     9961 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    10021 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    10081 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    10141 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    10201 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    10261 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    10321 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    10381 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    10441 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    10501 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    10561 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    10621 ta
//
