LOCUS       sequence-328066-       11579 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral plasmid expressing GFP-tagged SFPQ protein with 
            IRES-mCherry from the EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: pLV-EF1a-GFP-SFPQ-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..11579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3386..3395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3392..3394,3395..3397,3398..4108)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3392..3394,3395..3397,3398..4108)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3437..3458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3698..3717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4045..4066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6261..6812
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6405..6422)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             6812..7522
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6957..6975)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7225..7244
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7548..8136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7601..7621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8019..8030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8343..8976
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9104..9120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9104..9120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9117..9139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9128..9144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9152..9158,9159..9176,9177..9182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9197..9218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9335..9352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9506..10094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9586..9605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10265..11056,11057..11125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10888..10907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11126..11230)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11278..11412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11315..11334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11369..11388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcgccac catggtgagc aagggcgagg agctgttcac
     3421 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3481 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3541 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3601 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3661 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3721 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3781 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3841 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3901 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3961 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4021 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4081 cactctcggc atggacgagc tgtacaagat gtctcgggat cggttccgga gtcgtggcgg
     4141 tggcggtggt ggcttccaca ggcgtggagg aggcggcggc cgcggcggcc tccacgactt
     4201 ccgttctccg ccgcccggca tgggcctcaa tcagaatcgc ggccccatgg gtcctggccc
     4261 gggccagagc ggccctaagc ctccgatccc gccaccgcct ccacaccaac agcagcaaca
     4321 gccaccaccg cagcagccac cgccgcagca gccgccaccg catcagccgc cgccgcatcc
     4381 acagccgcat cagcagcagc agccgccgcc accgccgcag gactcttcca agcccgtcgt
     4441 tgctcaggga cccggccccg ctcccggagt aggcagcgca ccaccagcct ccagctcggc
     4501 cccgcccgcc actccaccaa cctcgggggc cccgccaggg tccgggccag gcccgactcc
     4561 gaccccgccg cctgcagtca cctcggcccc tcccggggcg ccgccaccca ccccgccaag
     4621 cagcggggtc cctaccacac ctcctcaggc cggaggcccg ccgcctccgc ccgcggcagt
     4681 cccgggcccg ggtccagggc ctaagcaggg cccaggtccg ggtggtccca aaggcggcaa
     4741 aatgcctggc gggccgaagc caggtggcgg cccgggccta agtacgcctg gcggccaccc
     4801 caagccgccg catcgaggcg gcggggagcc ccgcgggggc cgccagcacc acccgcccta
     4861 ccaccagcag catcaccagg ggcccccgcc cggcgggccc ggcggccgca gcgaggagaa
     4921 gatctcggac tcggaggggt ttaaagccaa tttgtctctc ttgaggaggc ctggagagaa
     4981 aacttacaca cagcgatgtc ggttgtttgt tgggaatcta cctgctgata tcacggagga
     5041 tgagttcaaa agactatttg ctaaatatgg agaaccagga gaagttttta tcaacaaagg
     5101 caaaggattc ggatttatta agcttgaatc tagagctttg gctgaaattg ccaaagccga
     5161 actggatgat acacccatga gaggtagaca gcttcgagtt cgctttgcca cacatgctgc
     5221 tgccctttct gttcgtaatc tttcacctta tgtttccaat gaactgttgg aagaagcctt
     5281 tagccaattt ggtcctattg aaagggctgt tgtaatagtg gatgatcgtg gaagatctac
     5341 agggaaaggc attgttgaat ttgcttctaa gccagcagca agaaaggcat ttgaacgatg
     5401 cagtgaaggt gttttcttac tgacgacaac tcctcgtcca gtcattgtgg aaccacttga
     5461 acaactagat gatgaagatg gtcttcctga aaaacttgcc cagaagaatc caatgtatca
     5521 aaaggagaga gaaacccctc ctcgttttgc ccagcatggc acgtttgagt acgaatattc
     5581 tcagcgatgg aagtctttgg atgaaatgga aaaacagcaa agggaacaag ttgaaaaaaa
     5641 catgaaagat gcaaaagaca aattggaaag tgaaatggaa gatgcctatc atgaacatca
     5701 ggcaaatctt ttgcgccaag atctgatgag acgacaggaa gaattaagac gcatggaaga
     5761 acttcacaat caagaaatgc agaaacgtaa agaaatgcaa ttgaggcaag aggaggaacg
     5821 acgtagaaga gaggaagaga tgatgattcg tcaacgtgag atggaagaac aaatgaggcg
     5881 ccaaagagag gaaagttaca gccgaatggg ctacatggat ccacgggaaa gagacatgcg
     5941 aatgggtggc ggaggagcaa tgaacatggg agatccctat ggttcaggag gccagaaatt
     6001 tccacctcta ggaggtggtg gtggcatagg ttatgaagct aatcctggcg ttccaccagc
     6061 aaccatgagt ggttccatga tgggaagtga catgcgtact gagcgctttg ggcagggagg
     6121 tgcggggcct gtgggtggac agggtcctag aggaatgggg cctggaactc cagcaggata
     6181 tggtagaggg agagaagagt acgaaggccc aaacaaaaaa ccccgatttt agggcgcgcc
     6241 ccatcccgcc cctctcccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6301 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6361 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6421 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     6481 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     6541 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     6601 tgtgagttgg atagttgtgg aaagagtcaa atggctcacc tcaagcgtat tcaacaaggg
     6661 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     6721 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     6781 gtggttttcc tttgaaaaac acgatgataa tatggtgagc aagggcgagg aggataacat
     6841 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     6901 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     6961 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     7021 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     7081 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     7141 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     7201 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     7261 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     7321 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     7381 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     7441 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     7501 catggacgag ctgtacaagt gaaacccggg cggggcgcgt ctggaacaat caacctctgg
     7561 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7621 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7681 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7741 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7801 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7861 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7921 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7981 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8041 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8101 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     8161 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     8221 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     8281 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
     8341 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     8401 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     8461 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     8521 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     8581 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     8641 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     8701 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     8761 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     8821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct
     9061 agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    11281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    11341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11401 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    11461 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    11521 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
