LOCUS       sequence-328156-        8964 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FRET analysis (pro-IL-1beta cleavage) in living cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Magupalli VG, Negro R, Tian Y, Hauenstein AV, Di Caprio G, Skillern 
            W, Deng Q, Orning P, Alam HB, Maliga Z, Sharif H, Hu JJ, Evavold CL,
            Kagan JC, Schmidt FI, Fitzgerald KA, Kirchhausen T, Li Y, Wu H
  TITLE     HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin 
            inflammasome activation.
  JOURNAL   Science. 2020 Sep 18;369(6510). pii: 369/6510/eaas8995. doi: 
            10.1126/science.aas8995.
  PUBMED    32943500
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-09-18"; volume: "369(6510). pii: 369/6510/eaas8995. 
            doi"; pages: " 10.1126/science.aas8995"
COMMENT     Sequence Label: pLV-mTurquoise2-IL-1beta-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2525..2527,2528..2530,2531..3241)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2525..2527,2528..2530,2531..3241)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2570..2591)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2831..2850)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3178..3199
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4091..4801
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4346..4355
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4808..5396
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4861..4881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5279..5290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5483..5716
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5788..5909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5825..5844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5879..5898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5949..6084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6104..6123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6105..6123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6133..6150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6133..6149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6142..6164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6291..6746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6378..6397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6588..6609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6772..6876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6877..6945,6946..7737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7095..7114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7908..8496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8397..8416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8650..8667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8784..8805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8820..8825,8826..8843,8844..8850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8858..8874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8863..8885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8882..8898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8882..8898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8917..8937
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8919..8937
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt atagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2581 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2641 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2701 caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
     2761 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2821 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2881 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2941 gcacaagctg gagtacaact acttcagcga caacgtctat atcaccgccg acaagcagaa
     3001 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     3061 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3121 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     3181 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3241 gtccggactc agatctcgag gaatggcaga agtacctgag ctcgccagtg aaatgatggc
     3301 ttattacagt ggcaatgagg atgacttgtt ctttgaagct gatggcccta aacagatgaa
     3361 gtgctccttc caggacctgg acctctgccc tctggatggc ggcatccagc tacgaatctc
     3421 cgaccaccac tacagcaagg gcttcaggca ggccgcgtca gttgttgtgg ccatggacaa
     3481 gctgaggaag atgctggttc cctgcccaca gaccttccag gagaatgacc tgagcacctt
     3541 ctttcccttc atctttgaag aagaacctat cttcttcgac acatgggata acgaggctta
     3601 tgtgcacgat gcacctgtac gatcactgaa ctgcacgctc cgggactcac agcaaaaaag
     3661 cttggtgatg tctggtccat atgaactgaa agctctccac ctccagggac aggatatgga
     3721 gcaacaagtg gtgttctcca tgtcctttgt acaaggagaa gaaagtaatg acaaaatacc
     3781 tgtggccttg ggcctcaagg aaaagaatct gtacctgtcc tgcgtgttga aagatgataa
     3841 gcccactcta cagctggaga gtgtagatcc caaaaattac ccaaagaaga agatggaaaa
     3901 gcgatttgtc ttcaacaaga tagaaatcaa taacaagctg gaatttgagt ctgcccagtt
     3961 ccccaactgg tacatcagca cctctcaagc agaaaacatg cccgtcttcc tgggagggac
     4021 caaaggcggc caggatataa ctgacttcac catgcaattt gtgtcttccg gatccaccgg
     4081 tagcggctca atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca
     4141 tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac
     4201 cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca
     4261 gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta
     4321 ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca
     4381 tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga
     4441 gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc
     4501 tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa
     4561 cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta
     4621 ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa
     4681 gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga ccgagctcaa
     4741 cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaagta
     4801 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4861 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4921 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4981 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5041 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5101 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5161 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5221 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5281 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5341 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     5401 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5461 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     5521 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5701 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5761 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     5821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5881 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     5941 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6001 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6061 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6121 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6181 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6241 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6301 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6361 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6421 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6481 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6541 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6601 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6661 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6721 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6781 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6841 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6901 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6961 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7021 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7081 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7141 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7201 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7261 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7321 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7381 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7441 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7501 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7561 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7621 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7681 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7741 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8041 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8701 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8761 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8821 ttccacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8881 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8941 caaaagctgg agctgcaagc ttaa
//
