LOCUS       sequence-328207-       10199 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expressing dCas9 and sgRNA containg MTSa targeting centromeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK
  TITLE     A CRISPR/molecular beacon hybrid system for live-cell genomic 
            imaging.
  JOURNAL   Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304.
  PUBMED    29718399
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2018-07-27"; volume: "46(13):e80. doi"; pages: "
            10.1093/nar/gky304"
COMMENT     Sequence Label: SL2-sgSat-MTSb/BFP/pdCas9-C1
FEATURES             Location/Qualifiers
     source          1..10199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..291
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     248..268
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(293..397)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      424..879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(511..530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     721..742
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    886..1007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1204..1224)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(1234..2817,2818..2820,2821..5307,5308..5310,
                     5311..5334))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5335..5355)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(5395..5598)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5599..5902
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(6157..6405)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6215..6234)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6385..6405)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    6409..6530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6654..6719
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(6720..7430)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      7427..7436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7475..7678)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        7679..7982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(8108..8696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8188..8207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9025..9072
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     9097..9116
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(9304..10098)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9304..10098)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9415..9434)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10025..10044
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(10133..10199,1..291))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10147..10199,1..83)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
       61 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
      121 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
      181 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      241 tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca ggactcttcc
      301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      361 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      421 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      481 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      541 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      601 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      661 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      721 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      781 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      841 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgcgttaaga tacattgatg
      901 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      961 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     1021 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     1081 acctctacaa atgtggtatg gctgattatg atcagttatc tagatccggt ggatcccggg
     1141 cccgcggtac cgtcgactgc agaattctta gaacagctcc tcgcccttgc tcacggaact
     1201 acctaccttg cgctttttct tgggagctcc ctcatcccct ccgagctgtg agaggtcaat
     1261 ccgagtttcg taaagtccgg tgatgctctg gtggatgagg gtggcgtcca gcacttcttt
     1321 ggtggaggta tagcgtttcc ggtcgatggt ggtgtcgaag tacttgaatg cggcaggggc
     1381 accaagattg gtgagggtga acaggtggat gatattctca gcctgctccc gaattggttt
     1441 gtctctgtgc ttattgtatg cgctaaggac tttatcgagg ttggcatcag cgagaatgac
     1501 gcgtttagaa aactcggaaa tctgctcaat gatctcgtcc agataatgct tatgctgctc
     1561 cacaaacagt tgcttttgct cgttgtcctc ggggctcccc ttcagcttct cgtaatggct
     1621 tgcaaggtag aggaaattca cgtacttgga ggggagagcc agctcattac ccttctgaag
     1681 ttcgccagcg gaggcgagca tccgcttccg cccattctcc agttcgaaca ggctatactt
     1741 tgggagcttg atgataaggt ccttcttgac ttccttgtaa cctttggctt cgagaaagtc
     1801 aattgggttc ttctcgaagg agctgcgctc cataatggtg atacccagca gttccttcac
     1861 gctcttgagc ttcttagact ttcctttttc caccttagcc acgaccagga cagaataagc
     1921 cacagttggt gaatcgaatc cgccgtactt cttagggtcc caatccttct tccttgcaat
     1981 gagcttgtcg gagttgcgct tggggaggat tgattcctta gaaaagcctc cggtttgcac
     2041 ctcggttttc ttcacgatgt tcacttgagg catagaaagc actttgcgca cggtagcgaa
     2101 atctctgccc ttgtcccaca cgatttcgcc agtctcccca ttagtctcga tgagcggcct
     2161 cttccggatc tcaccgtttg caagggtgat ttcggtcttg aagaaattca tgatgttgct
     2221 gtagaagaag tactttgcgg tggcctttcc gatctcctgc tcagatttgg caatcatctt
     2281 gcgcacgtcg tagaccttgt agtctccgta cacgaactcg ctttccagct ttgggtactt
     2341 cttgatcagg gcggttccca ccacggcatt cagatatgcg tcatgagcat gatgatagtt
     2401 attgatctcc cgcactttgt agaattgaaa gtcctttctg aaatcgctga ccagcttgga
     2461 cttcagggta atcaccttca cctcgcggat aagtttgtcg ttttcgtcgt acttggtatt
     2521 catgcgtgag tcaaggattt gggcgacatg tttagtaatc tgccgagtct ccacaagttg
     2581 ccgtttaatg aatcctgctt tgtccagttc gctcagtccg cccctctcag ccttagtgag
     2641 attgtcgaac ttccgctgag tgatcagctt tgcgttcaga agctggcgcc aatagttctt
     2701 catctttttc acgacttcct ctgagggcac gttatctgac tttccccggt tcttgtctga
     2761 gcgagtcagc accttattgt caattgagtc gtctttgagg aagctctgag ggacgatggc
     2821 gtccacgtcg tagtctgaga gcctgttgat atcaagctct tggtccacat acatatcgcg
     2881 tccattttga aggtagtaca gatagagctt ctcattctga agctgggtgt tttccaccgg
     2941 gtgctcttta aggatctgtg acccgagctc cttaatccct tcctcaattc ttttcatccg
     3001 ctccctgctg ttcttctgtc ccttctgggt agtctggttt tccctggcca tctcgatgac
     3061 aatgttctct ggtttatggc gacccatcac cttgaccagc tcgtccacga ctttcacggt
     3121 ctggagaata cccttcttaa tagcgggtga accagccaga tttgcgatat gctcgtgcag
     3181 tgagtcacct tgtccagaca cttgggcctt ctggatgtcc tccttaaagg tcagggaatc
     3241 atcatggata agttgcatga agttgcgatt agcgaatccg tcgctcttaa ggaaatcaag
     3301 aatggtcttt ccgctctgtt tgtcccggat tccgttgatg agtttgcggc taaggcgtcc
     3361 ccatccggta tatcttctcc gtttaagttg cttcatcact ttatcgtcga acagatgggc
     3421 gtaggtctta agcctctcct cgatcatttc ccggtcctcg aacagagtga gagtcaggac
     3481 aatgtcctca aggatgtcct cgttctcctc attgtccaga aaatccttgt ccttgatgat
     3541 cttaagcaga tcatggtagg tacccaggct tgcgttgaac cgatcttcga ccccgctaat
     3601 ctccacagag tcgaagcatt caattttctt gaaatagtcc tccttcagct gcttcacggt
     3661 cacctttcta ttggtcttga acagaaggtc cacaattgcc ttcttttgtt cgcctgacag
     3721 gaatgcgggc ttcctcatcc cttcggtgac atatttcacc ttggtcagct cgttgtacac
     3781 ggtaaagtac tcgtagagca gggaatgttt cggcaggacc ttctcattgg ggaggttctt
     3841 atcgaagtta gtcatccgtt cgatgaatga ctgagcgctg gctcccttgt ccaccacctc
     3901 ttcgaagttc cagggagtga tagtttcttc tgactttctg gtcatccaag caaaccggct
     3961 atttcctctg gcgaggggtc ccacgtagta ggggatgcgg aaagtgagaa ttttctcaat
     4021 cttctccctg ttgtccttaa gaaaggggta gaagtcctct tggcgccgaa ggatggcgtg
     4081 aagctcccca agatggatct ggtggggaat gctcccattg tcgaaagtgc gctgcttccg
     4141 cagcagatcc tcccgattca gtttcacgag gagctcttcg gtgccgtcca tcttctcaag
     4201 gataggtttg ataaacttat aaaattcctc ttgggaggca cctccgtcga tgtagccggc
     4261 gtatccattc tttgactggt caaagaagat ttccttgtac ttctccggaa gctgctgccg
     4321 gacgagtgcc ttaagcaggg tcaggtcctg gtggtgctca tcataccgct taatcattga
     4381 agctgaaaga ggggccttgg tgatttcagt gttcacgcgg agaatgtcag acagcaggat
     4441 ggcgtcagaa agattcttag cagcaaggaa aaggtcggcg tattggtcac cgatctgggc
     4501 caggaggttg tcgagatcgt catcataggt gtccttggac agttgcagct tggcatcctc
     4561 tgccaggtcg aaattagact tgaaattggg agtcagccca agtgaaagag caatcaggtt
     4621 cccgaacagt ccatttttct tttcacccgg cagctgagcg ataagattct caagtctgcg
     4681 gctctttgac agccgggctg acagaatggc cttggcatcg actccgcttg cattgatggg
     4741 gttctcttca aagagctggt tgtaagtctg caccagctgg atgaacagtt tatccacatc
     4801 gctattgtca ggattgaggt cgccctcgat caggaagtgg cctctgaact tgatcatgtg
     4861 ggccagggcc aggtagataa gccggaggtc tgccttgtcg gttgagtcca ccagcttttt
     4921 gcgaagatgg tagatggttg ggtatttctc gtgataggcc acctcgtcga cgatattacc
     4981 gaagatggga tgccgttcat gcttcttatc ctcttccacg aggaatgatt cttccagcct
     5041 atggaagaag ctgtcgtcga cctttgccat ttcgttggag aatatctctt gaaggtaaca
     5101 aatccggttc ttcctcctgg tgtaccgccg tctagcagtc cgcttgaggc gggttgcttc
     5161 agcggtctcc cctgaatcaa agaggagagc tccgatcaga tttttcttga tggaatgccg
     5221 gtcggtgttc ccgagcacct tgaacttctt agagggcacc ttgtactcgt cagtgatcac
     5281 ggcccacccg acgctattag tcccgatggc cagtccgata gaatacttct tgtccacttt
     5341 cctcttcttt ttgggcacca tggtgggcta gcggatctga cggttcacta aaccagctct
     5401 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     5461 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     5521 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     5581 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     5641 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     5701 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     5761 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     5821 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     5881 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     5941 aattgattac tattactcgt cggtcccggc atccgatatt gggtcaaaaa aagcaccgac
     6001 tcggtgccac tggccccgga gcagactgca gctactcagc gtaagtgatg tcgtgacgtc
     6061 ttctgctccg gggccaagtt gataacggac tagccttatt tgaacttgct atgctgtttc
     6121 cagcatagct ctcaaacaat atccacttgc agattcggtg tttcgtcctt tccacaagat
     6181 atataaagcc aagaaatcga aatactttca agttacggta agcatatgat agtccatttt
     6241 aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt
     6301 ttgtactaat atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt
     6361 gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctctaata agatacattg
     6421 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6481 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6541 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     6601 aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatccc
     6661 gggcccgcgg taccgtcgac tgcagaattc gaagcttgag ctcgagatct gagtccggaa
     6721 ttaagcttgt gccccagttt gctagggagg tcgcagtatc tggccactgc cacctcgtgc
     6781 tgctcgacgt aggtctcgtt gttggcctcc ttgattcttt ccagtctgta gtccacatag
     6841 tagacgccag gcatcttgag gttcttagcg ggtttcttgg atctatatgt ggtcttggcg
     6901 tttgcgatca gatggctccc gcccacgagc ttcagggcca tgtcgtttct gccttccagg
     6961 ccgccgtcag cggggtacag cgtctcggtg aaggcctccc agccgagtgt tttcttctgc
     7021 atcacagggc cgttggatgt gaagttcacc cctctgatct tgacgttgta gatgaggcag
     7081 ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc ccccgtcttc gtatgtggtg
     7141 actctctccc atgtgaagcc ctcagggaag gactgcttga agaagtcggg gatgccctgg
     7201 gtgtggttga tgaaggtctt gctgccgtag aggaagctag tagccaggat gtcgaaggcg
     7261 aaggggagag ggccgccctc gaccaccttg attctcatgg tctgggtgcc ctcgtagggc
     7321 ttgccttcgc cctcggatgt gcacttgaag tgatggttgt ccacggtgcc ctccatgtac
     7381 agcttcatgt gcatgttctc cttaatcagc tcttcgccct tagacaccat ggtggcgacc
     7441 ggtagcgcta gcggatctga cggttcacta aaccagctct gcttatatag acctcccacc
     7501 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     7561 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     7621 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     7681 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     7741 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     7801 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     7861 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     7921 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     7981 cgcgtaataa ctaatgcatg gcggtaatac ggttatccac agaatcaggg gataacgcag
     8041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8701 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8821 gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc
     8881 cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg
     8941 aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc
     9001 cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc
     9061 tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta
     9121 gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     9181 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     9241 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     9301 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     9361 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     9421 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     9481 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     9541 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     9601 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     9661 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     9721 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     9781 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     9841 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     9901 ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     9961 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
    10021 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
    10081 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa
    10141 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcg
//
