LOCUS       sequence-328248-       10472 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from 
            the EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: pLV-EF1a-HaloSFPQY527A
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3386..3395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3392..4279
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     misc_feature    6441..7029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6494..6514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6912..6923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7236..7869
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7997..8013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7997..8013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8010..8032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8021..8037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8045..8051,8052..8069,8070..8075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8090..8111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8228..8245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8399..8987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8479..8498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9158..9949,9950..10018))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9781..9800
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10019..10123)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10171..10305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10208..10227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10262..10281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatcgccac catggaaatc ggtactggct ttccattcga
     3421 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga
     3481 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat
     3541 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa
     3601 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt
     3661 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct
     3721 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt
     3781 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc
     3841 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
     3901 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
     3961 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
     4021 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
     4081 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
     4141 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
     4201 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
     4261 tactctggag atttccggtg gcgccccgta caagatgtct cgggatcggt tccggagtcg
     4321 tggcggtggc ggtggtggct tccacaggcg tggaggaggc ggcggccgcg gcggcctcca
     4381 cgacttccgt tctccgccgc ccggcatggg cctcaatcag aatcgcggcc ccatgggtcc
     4441 tggcccgggc cagagcggcc ctaagcctcc gatcccgcca ccgcctccac accaacagca
     4501 gcaacagcca ccaccgcagc agccaccgcc gcagcagccg ccaccgcatc agccgccgcc
     4561 gcatccacag ccgcatcagc agcagcagcc gccgccaccg ccgcaggact cttccaagcc
     4621 cgtcgttgct cagggacccg gccccgctcc cggagtaggc agcgcaccac cagcctccag
     4681 ctcggccccg cccgccactc caccaacctc gggggccccg ccagggtccg ggccaggccc
     4741 gactccgacc ccgccgcctg cagtcacctc ggcccctccc ggggcgccgc cacccacccc
     4801 gccaagcagc ggggtcccta ccacacctcc tcaggccgga ggcccgccgc ctccgcccgc
     4861 ggcagtcccg ggcccgggtc cagggcctaa gcagggccca ggtccgggtg gtcccaaagg
     4921 cggcaaaatg cctggcgggc cgaagccagg tggcggcccg ggcctaagta cgcctggcgg
     4981 ccaccccaag ccgccgcatc gaggcggcgg ggagccccgc gggggccgcc agcaccaccc
     5041 gccctaccac cagcagcatc accaggggcc cccgcccggc gggcccggcg gccgcagcga
     5101 ggagaagatc tcggactcgg aggggtttaa agccaatttg tctctcttga ggaggcctgg
     5161 agagaaaact tacacacagc gatgtcggtt gtttgttggg aatctacctg ctgatatcac
     5221 ggaggatgag ttcaaaagac tatttgctaa atatggagaa ccaggagaag tttttatcaa
     5281 caaaggcaaa ggattcggat ttattaagct tgaatctaga gctttggctg aaattgccaa
     5341 agccgaactg gatgatacac ccatgagagg tagacagctt cgagttcgct ttgccacaca
     5401 tgctgctgcc ctttctgttc gtaatctttc accttatgtt tccaatgaac tgttggaaga
     5461 agcctttagc caatttggtc ctattgaaag ggctgttgta atagtggatg atcgtggaag
     5521 atctacaggg aaaggcattg ttgaatttgc ttctaagcca gcagcaagaa aggcatttga
     5581 acgatgcagt gaaggtgttt tcttactgac gacaactcct cgtccagtca ttgtggaacc
     5641 acttgaacaa ctagatgatg aagatggtct tcctgaaaaa cttgcccaga agaatccaat
     5701 gtatcaaaag gagagagaaa cccctcctcg ttttgcccag catggcacgt ttgagtacga
     5761 atattctcag cgatggaagt ctttggatga aatggaaaaa cagcaaaggg aacaagttga
     5821 aaaaaacatg aaagatgcaa aagacaaatt ggaaagtgaa atggaagatg ccgctcatga
     5881 acatcaggca aatcttttgc gccaagatct gatgagacga caggaagaat taagacgcat
     5941 ggaagaactt cacaatcaag aaatgcagaa acgtaaagaa atgcaattga ggcaagagga
     6001 ggaacgacgt agaagagagg aagagatgat gattcgtcaa cgtgagatgg aagaacaaat
     6061 gaggcgccaa agagaggaaa gttacagccg aatgggctac atggatccac gggaaagaga
     6121 catgcgaatg ggtggcggag gagcaatgaa catgggagat ccctatggtt caggaggcca
     6181 gaaatttcca cctctaggag gtggtggtgg cataggttat gaagctaatc ctggcgttcc
     6241 accagcaacc atgagtggtt ccatgatggg aagtgacatg cgtactgagc gctttgggca
     6301 gggaggtgcg gggcctgtgg gtggacaggg tcctagagga atggggcctg gaactccagc
     6361 aggatatggt agagggagag aagagtacga aggcccaaac aaaaaacccc gattttagcc
     6421 gggcggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6481 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6541 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6601 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6661 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6721 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6781 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6841 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6901 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6961 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7021 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     7081 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     7141 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7201 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     7261 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     7321 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     7381 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     7441 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     7501 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7561 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     7621 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     7681 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7741 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7801 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7861 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7921 gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga
     7981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10141 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    10201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10321 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10381 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10441 atttgttaaa tatgtactac aaacttagta gt
//
