LOCUS       sequence-329678-        4208 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Constitutively expressed, chimeric transcription activator 
            comprising the AraC DNA binding domain and the RhaR rhamnose ligand 
            binding domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: RRRAA-pHG165c
FEATURES             Location/Qualifiers
     source          1..4208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..72
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(153..170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(324..912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(404..423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1083..1874,1875..1943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1706..1725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1944..2048)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2116..2134
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2287..2309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2301..2318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2302..2318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3913..4104
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4098..4120)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    join(4206..4208,1..138)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
       61 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      121 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
      781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2041 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2101 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga atccgttaac
     2161 ggcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2221 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2281 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttggc tgcaggtcga
     2341 cggatccccg ggaattccac catcgaatgg tgacaccatc gaatggtgca aaacctttcg
     2401 cggtatggca tgatagcgcc cggggcccaa gttcacttaa aaaggagatc aacaatgaag
     2461 caattttcgt actgaaacat cttaatcatg ctggggaggg tttctaatgg cgcatcagtt
     2521 aaaacttctc aaagatgatt tttttgccag cgaccagcag gcagtcgctg tggctgaccg
     2581 ttatccgcaa gatgtctttg ctgaacatac acatgatttt tgtgagctgg tgattgtctg
     2641 gcgcggtaat ggcctgcatg tactcaacga tcgcccttat cgcattaccc gtggcgatct
     2701 cttttacatt catgctgacg ataaacactc ctacgcttcc gttaacgatc tggttttgca
     2761 gaatattatt tattgcccgg agcgtctgaa gctgaatctt gactggcagg gggcgattcc
     2821 gggatttaac gccagcgcag ggcaaccaca ctggcgctta ggtagcatgg ggatggcgca
     2881 ggcgcggcag gttatcggtc agcttgagca tgaaagtagt cagcatgtgc cgtttgctaa
     2941 cgaaatggct gagttgctgt tcgggcagtt ggtgatgttg ttgaatcgcc atcgttacaa
     3001 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga
     3061 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc
     3121 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga
     3181 ggaccaacgt atcagccagg cgaagctgct tttgagcacc acccggatgc ctatcgccac
     3241 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgggtat ttaaaaaatg
     3301 caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc
     3361 cgtcaagttg tcataatgag aattcgtaat aatatagctc tagattaatt gcgcccgtta
     3421 acggcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     3481 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     3541 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     3601 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
     3661 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     3721 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
     3781 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     3841 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     3901 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     3961 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     4021 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     4081 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4141 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4201 cggggcgc
//
