LOCUS       sequence-329917-       10167 bp    DNA     circular SYN 14-APR-2025
DEFINITION  This Cre-ERT2 expressing construct can be used to inducibly 
            recombine loxp sites. It can be used with poly-loxP containing 
            plasmids to generate timestamp barcodes useful for linage tracing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Kim IS, Wu J, Rahme GJ, Battaglia S, Dixit A, Gaskell E, Chen H, 
            Pinello L, Bernstein BE
  TITLE     Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage 
            Decisions in Developing Embryoid Bodies.
  JOURNAL   Cell Rep. 2020 Oct 6;33(1):108222. doi: 
            10.1016/j.celrep.2020.108222.
  PUBMED    33027665
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-10-6"; volume: "33(1):108222. doi"; pages: " 
            10.1016/j.celrep.2020.108222"
COMMENT     Sequence Label: p-EF1a-CreERT2-3Xflag-T2A-eBFP2
FEATURES             Location/Qualifiers
     source          1..10167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4144..5321
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4375..5312
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5270..5290
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5383..6411
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             6424..7359
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             7369..7434
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7441..7494
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7507..7509,7510..8223)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKV
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     misc_feature    8230..8818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8283..8303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8701..8712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8905..9138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9210..9331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9247..9266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9301..9320
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9371..9506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9526..9545)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9527..9545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9555..9572)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9555..9571)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9564..9586)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9713..10141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9800..9819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10010..10031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gatggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4201 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4261 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4321 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     4381 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     4441 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     4501 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     4561 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     4621 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     4681 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     4741 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     4801 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     4861 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     4921 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     4981 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     5041 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     5101 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     5161 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     5221 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     5281 acagtggttc aaagtttttt cttccatttc aggtgtcgtg agcctggaga cgccatccac
     5341 gctgttttga cctccataga agacaccgac tctagagcca ccatgtccaa tttactgacc
     5401 gtacaccaaa atttgcctgc attaccggtc gatgcaacga gtgatgaggt tcgcaagaac
     5461 ctgatggaca tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg
     5521 tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata accggaaatg gtttcccgca
     5581 gaacctgaag atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa
     5641 actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga
     5701 ccaagtgaca gcaatgctgt ttcactggtt atgcggcgaa tccgaaaaga aaacgttgat
     5761 gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt
     5821 tcactcatgg aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt
     5881 gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca
     5941 cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc
     6001 gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc
     6061 gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt
     6121 gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa
     6181 gcaactcatc gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg
     6241 tctggacaca gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata
     6301 ccggagatca tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt
     6361 aacctggata gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga tctcgagcca
     6421 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
     6481 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
     6541 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
     6601 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
     6661 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tcttgaatgt
     6721 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
     6781 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
     6841 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
     6901 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
     6961 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
     7021 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     7081 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     7141 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     7201 gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     7261 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     7321 tattacatca cgggggaggc agagggtttc cctgccacag ctggatccga ctacaaagac
     7381 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagagatcc
     7441 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctagtaca
     7501 actagcgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     7561 gacggcgacg taaacggcca caagttcagc gtgaggggcg agggcgaggg cgatgccacc
     7621 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     7681 accctcgtga ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     7741 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     7801 ttcttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     7861 ctagtgaacc gcatcgagct gaagggcgtc gacttcaagg aggacggcaa catcctgggg
     7921 cacaagctgg agtacaactt caacagccac aacatctata tcatggccgt caagcagaag
     7981 aacggcatca aggtgaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc
     8041 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacagc
     8101 cactacctga gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg
     8161 gtcctgctgg agttccgcac cgccgccggg atcactctcg gcatggacga gctgtacaag
     8221 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8281 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8341 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8401 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8461 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8521 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8581 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     8641 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8701 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8761 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     8821 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8881 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8941 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9001 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9061 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9121 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     9181 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     9241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     9361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9481 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     9541 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9601 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9661 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     9721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9901 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10021 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10081 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10141 tttaacaaaa tattaacgct tacaatt
//
