LOCUS       sequence-33016-e         882 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, 
            Roberts AG, Oparka KJ, Christie JM
  TITLE     The photoreversible fluorescent protein iLOV outperforms GFP as a 
            reporter of plant virus infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 16. 105(50):20038-43.
  PUBMED    19060199
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 16. 105(50):20038-43."
COMMENT     Sequence Label: pUC57 iLOV ANAB
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(425..441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(425..441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(438..460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    449..465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(473..479,480..497,498..503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    518..539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(656..673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaccggtcct cgcgaangca tctagaatcc aatattgaaa aaaattttgt tattactgat
       61 ccacgtttac cagataatcc aattattttt gctagtgatg gttttttaga attaactgaa
      121 tatagtcgtg aagaaatttt aggtcgtaat gctcgttttt tacaaggtcc agaaactgat
      181 caagctactg ttcaaaaaat tcgtgatgct attcgtgatc aacgtgaaac tactgttcaa
      241 ttaattaatt atactaaaag tggtaaaaaa ttttggaatt tattacattt acaaccagtt
      301 cgtgatcaaa aaggtgaatt acaatatttt attggtgttc aattagatgg tagtgatcat
      361 gttccaaatc ggatcccggg cccgtcgact gcagaggcct gcatgcaagc ttggcgtaat
      421 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      481 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      541 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      601 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      721 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca ga
//
