LOCUS       sequence-330513-        3380 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Constitutive expression vector for Escherichia coli. BglBrick 
            compatible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, 
            Breitling R, Takano E, Scrutton NS
  TITLE     SelProm: A Queryable and Predictive Expression Vector Selection Tool
            for Escherichia coli.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399.
  PUBMED    30870592
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399."
COMMENT     Sequence Label: pBbB19a-GFP
FEATURES             Location/Qualifiers
     source          1..3380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     CDS             81..797
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(111..139)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     734..754
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      830..901
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      917..944
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1011..1673)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(2260..2354)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2384..3175,3176..3244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3007..3026
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3245..3349)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagcaggtt taatcgaatt caaaagatct
       61 tttaagaagg agatatacat atgagtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccgtg gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcccgtt
      301 atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaacgcac tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct cggacacaaa ctggagtaca actataactc acacaatgta tacatcacgg
      541 cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg
      601 gctccgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg
      781 atgagctcta caaataagga tccaaactcg agtaaggatc tccaggcatc aaataaaacg
      841 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      901 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata cctaggctac
      961 agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc
     1021 ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca
     1081 tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct
     1141 ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg
     1201 gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc
     1261 aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg
     1321 cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccaacacg
     1381 gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact
     1441 gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc
     1501 accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg
     1561 ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag
     1621 tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc
     1681 tccatgcggt aggggtgccg cacggttgcg gcaccatgcg caatcagctg caacttttcg
     1741 gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga ttttctttaa
     1801 cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc cccctttttt
     1861 aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt ggtgcccaaa
     1921 gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc cggcaaaaag
     1981 tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca aggtggcgct
     2041 gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc
     2101 gcccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
     2161 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
     2221 gaagcgcgca ggccgcaggc cggaggcact agtgcttgga ttctcaccaa taaaaaacgc
     2281 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     2341 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     2401 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2461 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2521 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2581 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2641 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2701 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2761 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2821 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2881 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2941 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3001 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3061 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3361 gaaaagtgcc acctgacgtc
//
