LOCUS       sequence-330767-       10145 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS9: pTns(AvCAST)_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     554..573
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(593..670)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        716..734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    735..759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5588..5606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5607..5631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7606..7624)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7620..7667
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7897..8712)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7897..8712)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8620..8639
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8713..8804)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8822..9457)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(8967..8986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9655..10145,1..592))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
       61 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
      121 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
      181 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
      241 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
      301 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
      361 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
      421 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
      481 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
      541 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
      601 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
      661 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata
      721 cgactcacta taggggaatt gtgagcggat aacaattccc ctgtagaaat aattttgttt
      781 aactttaata aggagatata ccatggttag gggtagaagg gaatggactc aagctaaata
      841 tgagcgatat ataaaagaag gtcgtggccg aggaagtggt aagcactata aaccttggct
      901 tacaatccaa gatgttccct ctaaaggacg ttcttctaga actcctggtt ggaaaacaaa
      961 tcgagtacat catttcttct cagaccatga aaaacggcta ttttatttgt ttgagtggtc
     1021 agatatagtc acagatataa gagagcaatt tcctttagac aatctcgatc tagccatgag
     1081 tattgctaca gacataggta taaagtaccc tgtagataca caaagtggca ctcctcacgt
     1141 tttaacaagc gactttatgc ttacagtcaa ccgcgatggg aagcagatgc agattgcccg
     1201 gactgttaag ccgtcaacag aactggagaa aaagcaagtt aatgagaaat tcgagataga
     1261 aagacaatat tacttagcaa agggtattga ctggggaatt atcacagaaa aagaaattcc
     1321 caggctttta gctgaaaatg ttgagtgggt tcattctgct tataggctag aagcaaattc
     1381 tgaaatggac actgcacaac tgagtgaatt gtcaacaatt ttaaaatcta agcttcagga
     1441 agaagataca actataaata gagtaactac tgctttagac aaagaaatga atataaattc
     1501 cggtacatca ctttatgtat ttaaacattt aattgctagg aaagaaatcc ttgtggatct
     1561 agtagcaata aaattatctg cttgtccgtc aactaaagtt attgaaaaaa taatatttta
     1621 gacccacatg ataggtaaat aatatggtta aagatttatt tgtcaatgat ttaatagagt
     1681 ggattgtata ccaaaacact catatagtcg aaagaattat ttggatagat gaaggttaca
     1741 ttattgcatt tgttatagat attaatgcta agaaaggtct acctgaacca aggaaaatat
     1801 cagatatttt agaagctata actgaaggta ttgcttttaa gcagcaacaa gatccttggg
     1861 caagaattat tcgggatgaa aatttgacag ataaggaaaa ggaatacaga gataaagctt
     1921 gggagataat ttcatcatta attatagaag aaccatcaat atattataga aattttagag
     1981 gatcgatggt taaagaagcg gttgaaaagt acaacgaaga aagggataaa gataaattag
     2041 tagaaaaaac cgtctatggt tatttgagaa gattttggca gagaggaaaa actataaatg
     2101 ctctcatacc tgattttatc aattcatcag gtaagggcaa gattcgagga caatcgggca
     2161 agaagagagg aagaccaaga aaatatgcac atgacccaga tattggtgaa ggtgtaaatg
     2221 ttacagaaga agatagaaag atttttagag tatctataag caaattttac aataatccaa
     2281 aagaaaacta cttaacaaca gcctatgaat taatggtgaa agaatattac aaggaaggaa
     2341 ttagatatga cgagaatggt gttcaaaaat ctatattaac cccaactgat aaaataccta
     2401 caattactca attcaaatac tggtatcaac ttgaacagga tgatgttacc aaaactttaa
     2461 catctcgaaa aggagccaag cgagtagctt tagagaatag agcaattttg ggaaactcta
     2521 agatggaaac tattggccct ggttctagat atcaaattga tgccacagtt gctgatgtat
     2581 atttagtttc aaaatataac cgtgactgga taattggtag accagttatc tatgtaatta
     2641 ttgatgtttt tagtagaatg attacaggtg tttatgtcgg tttagaaggc ccatcttgga
     2701 taggagcaat gatggcacta gccaatgtag ctacagataa aaccctgttt tgccaggaat
     2761 ataacattaa tattactgaa aatgaatggc cttgcaatca aatgccagat gcaattttag
     2821 gagatagggg agaattagca ggtatgacag tagaaacatt aatccctaat ctttatgttc
     2881 gtattgaaaa tgcagcttcc tatagagcag attggaaagg gttagtagaa agacactttc
     2941 gtataattca tgaatatgta aaaccttttt tacctggtta tgtggatacg gattttaggc
     3001 aaagaggtgc aagggattat aggcttgatg gcaaacttga tatagatcaa tttactgaga
     3061 ttattattcg tttaattgtt caccataaca ataaacctct tactgaatat gaaagagatg
     3121 aagcaatgat tgctgatgat acccctccta tacctagaga gttgtggaag tggggtatcg
     3181 ccaatcgttc tggcagactc agaacttttt ctgaagatat tgtcaaattg aacttgatgc
     3241 ctactgagaa agcaactatt actgctaaag gcatcaaaat gagaggtaag gagatgtact
     3301 atacctgtga tagtgctaaa aatgagcaat ggtttgaaaa agctagaagt aatctccttt
     3361 ctaagtcaga aaaatcattg attgtatctt atgacatcag aaagccaaac ttcatttatc
     3421 ttccatctag tgatggtaga gattttgaaa aatgttttct tattgactta gaagggcggt
     3481 atgctaataa aaactttcat gatattgaat atctgcttgc ttatgaagaa ttgcaaagac
     3541 agaaaagcca aggtaaacag ctacaagaaa aggtagattt aatagctgat attgaaagca
     3601 tagtagatag agcagaaaaa atgactgatg cttctcaata tcataattta agtaagagca
     3661 agaaagtttc tggaataaga gataataggg cttttgaaaa ggctaaacga cgagagaatg
     3721 aaggatttga attagcaatt acagataatc agagcagttt tcaggttgca gagagtacag
     3781 cagaaacctc cgaacagcca gaatcactac agccaaacca tctgaatctt cttaagaaaa
     3841 agcgacagga gcgaaaacgt ggacagaatt gagagtgaac tggttaaaat tcctaatgga
     3901 gaactagcag ttattgccag atatatagat caaaaactgc cagaatacaa cactaatcca
     3961 ttaattcagg cattacctcc tatcctatca gcagcagaat ttatagataa agtagccaga
     4021 gaaccaaatt ttgatgaaca agagagagag ctagaaccac attatagatt tcattgtatt
     4081 gagaggttat ctagatattt tgaccctcaa aataaaactc ttgaattaca aaaagttatt
     4141 tgtacgctta tcatgacagg ctatctgtca aggaaccttt taaaacctga atatgcaaat
     4201 cgctctaggc aaatttataa tgcaataggt aatggaggag gtaaaaattt agaaaactat
     4261 gttaatcttc ctacatctgc gtccggctta actctaattg gcccatcagg tattggaaag
     4321 actacaaatt tcaaaaatat tcttgagctt tatccccaaa taatttttca tcctgattat
     4381 agtgtttacc aaatagtttg gctgcgggta gattgcccta aaactttaaa ggggttatgt
     4441 atagatactt ttgcaggtat cgataaattg ttaggaacta atttattaaa gaaatttaac
     4501 ccagcacgca catctgaaga ttatctattg gctcaacttg cacaaacagc acacactcat
     4561 catctaggag ttttggtgat tgatgaaata caaaatttag caactgcaaa aagaaataga
     4621 gacgaaatat taaatttttt agtaaagctc gataatacta ttggaattcc agttattcgt
     4681 gtaggtacga atgaagccat tagttttctt caaggtaatc ttagaaatgc tagaagaaat
     4741 actggagaag gaagtagaat gtgggataga atggtaaata atgatgaatg gtgttacttt
     4801 ttagaaggaa tatgggaata ccaatggaca aaaacacctg ttcatttctc ggatgaaata
     4861 aaagaagttt tttatgatga aagccaggga attattgata ttgctataaa actttacaaa
     4921 atggttcaat ggagagcgat ttcaataggt gaccaagaaa ttattacggt tgatctgatt
     4981 cgtcaagtag ctaaagaagg attgtattta gtaaagccaa tgctagatgc aattaggtta
     5041 ggggacagag agtggatgat taaatataag gatattgctc ctctcgatat tacggaatat
     5101 cacaatagat gtgtatctga gattgatgct aaagatttga cagagattaa gagattagct
     5161 aaaaagcagc agaatcagca gaaagtatct ccgaagctca attatgtaat tagtgagctg
     5221 ttgaaattgg aagtagaatc tggtaaagca aaggaatgtg cagaacaaat tgtttatagt
     5281 catgaagatc cagacattcc aattccatct ttagttaata aggcatatgc tcttgctttg
     5341 caaggatgtc aagtaaatga gagtcttgct aaaacaagta ccaggaaagc taaattaaag
     5401 acaaagtacc aagagaatga tattcggcga attgtcgaga atgccaagaa agacaaaaaa
     5461 tctgcatacg aggatttgaa attagcaggt ataatcaaaa atccagttga agattttatc
     5521 aatatttagc ttctaactta agtcgaacag aaagtaatcg tattgtacac ggccgcataa
     5581 tcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc cccatcttag
     5641 tatattagtt aagtataaga aggagatata catggtgagt tttttccctc aaccttatcc
     5701 agatgaaatt ttatatagtg ttattgctcg ttatcacatt agaagtggca atacgagtcc
     5761 aaaaattaca ttacaagatt tatttaagtc acagactact gtggcgacag tagatttacc
     5821 atcgaacctt agtaatttaa ttaaaaacct acaatttatc tcaaattacc aagttgaaga
     5881 gttgatatat aaacatactc tctacccttt atatagccct tttttgccag caaacaggtc
     5941 tagtcaggta ctggaatcta tgaaaggaga ttatggggga gatattcata caagagccgg
     6001 aattatggca agctcaatta ctatgccgaa atatttgcgg ttttgtccaa cttgctggga
     6061 agaagactta aaaaattatg gcgaacctta ctggcatcgc atccatcaaa taccagggat
     6121 tttagtttgt ccatttcatg gtgaagtttt gcaagatagc ctagctcctt tacatggttt
     6181 taataagcat gaatattatg cagctagtac agacaactgt tctattacta aagaacccag
     6241 tattcttagc cctgacacac tgaaaaaatt actaatatta gccaaagatt tttggtggtt
     6301 actcaatagt gaaatacctt gccaagagcc acagtggttt cgcaaacagt acacaaatct
     6361 gatgattgag cagggttttg caactccaac tggtcgaatt taccagaaca aactactaga
     6421 taattttttg ttcttttatg gtcaagaaat gctcagtgct gttgattcaa tggtaaatca
     6481 taatgattca aacaactggg ttactagcat cgtcaggaaa tataggaaat ctttccatcc
     6541 tcttagacat ctgctaatga tgcgtttttt gaaaacatca gtagaggaat tttttcaaaa
     6601 agatactgaa tacaagcctt ttggcgaacc cccctggttg tgtctcaatg ctgccgcaga
     6661 acattatcta caaccagtga ttaccaattt agtcattact ttatgctgcg atacaaaaaa
     6721 gcctgtcggt actttttctt gtgcttgcgg aatggtttat tgccggactg ggcctgatga
     6781 aacggatgaa gacaaagttc gcattggtaa agtcaaggca ttcggtcaaa tatgggagca
     6841 aaagctgaaa gaattggtgg aagttgaaaa gttgggtttg cgggagacag ccagaagatt
     6901 aaatgttgat gccagaacta tcaaacgtta tgtatctcgt ctaaaattga caacttattg
     6961 gcagactaga acagaaaatg actctgtagg cttacaggga gtcccagaaa tagatcccaa
     7021 ttttgcaact gaactcaaat taaaacacag gcaaaattgg atggctttac aagtacagaa
     7081 ccctgaagct tcaaagacaa ctctgagaaa actagtacca gccacctata tttggctata
     7141 caggaatgac agagaatggc tcaatcaaaa ctcacccact ttacaagttg ccgttccttc
     7201 cgttgtgaga gttgattggc atgagcgaga tcagcaaatt ctcgctcagg ttcaagatgc
     7261 agtgcgatcg ctacttaagg ctgatatacc agagaaaatt tctattagta gggtagggaa
     7321 aactatcggc ttacttgcat tactcgaaaa acatctcgac cagatgcctt taacaaaggc
     7381 ttatttggaa tcagtcgttg aaactgtaga agaatttcag atgcgccgga tcaagtgggc
     7441 aatcaagctg cttgatgaat gtggagaaga aattatgcgc tggaaggtag tcagagttgc
     7501 tgctttgcga gaggattgct cccaaagagt cagtgcattt ctggaaagtg aactggagaa
     7561 agcatacaag aaagagaggt gattaattaa cctaggctgc tgccaccgct gagcaataac
     7621 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg
     7681 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     7741 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc
     7801 tccgcaagtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7861 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     7921 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     7981 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     8041 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     8101 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     8161 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     8221 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     8281 gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     8341 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     8401 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     8461 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     8521 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     8581 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     8641 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     8701 aatatggctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggca tgctagcgca
     8821 gaaacgtcct agaagatgcc aggaggatac ttagcagaga gacaataagg ccggagcgaa
     8881 gccgtttttc cataggctcc gcccccctga cgaacatcac gaaatctgac gctcaaatca
     8941 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg atggctccct
     9001 cttgcgctct cctgttcccg tcctgcggcg tccgtgttgt ggtggaggct ttacccaaat
     9061 caccacgtcc cgttccgtgt agacagttcg ctccaagctg ggctgtgtgc aagaaccccc
     9121 cgttcagccc gactgctgcg ccttatccgg taactatcat cttgagtcca acccggaaag
     9181 acacgacaaa acgccactgg cagcagccat tggtaactga gaattagtgg atttagatat
     9241 cgagagtctt gaagtggtgg cctaacagag gctacactga aaggacagta tttggtatct
     9301 gcgctccact aaagccagtt accaggttaa gcagttcccc aactgactta accttcgatc
     9361 aaaccgcctc cccaggcggt tttttcgttt acagagcagg agattacgac gatcgtaaaa
     9421 ggatctcaag aagatccttt acggattccc gacaccatca ctctagattt cagtgcaatt
     9481 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
     9541 ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     9601 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     9661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9721 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     9781 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     9841 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     9901 gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga
     9961 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
    10021 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
    10081 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
    10141 agcca
//
