LOCUS       sequence-330868-        9595 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et 
            al. Nucleic Acids Res 2019) fused to iRFP670.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, 
            Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z
  TITLE     Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection 
            in a Cyclophilin A-Dependent Manner.
  JOURNAL   mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 
            10.1128/mBio.03142-20.
  PUBMED    33758083
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-23"; volume: "12(2). pii: mBio.03142-20. doi"; pages:
            " 10.1128/mBio.03142-20"
COMMENT     Sequence Label: pSICO-CPSF6-4Glu-iRFP670
FEATURES             Location/Qualifiers
     source          1..9595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             211..391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    438..563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1060..1293
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1478..1522
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1820..1937
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2227..2247
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(2265..2298)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        2398..2701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2702..2905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4759..4768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4765..5700
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     protein_bind    complement(5709..5742)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     5769..5785
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5860..6448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5913..5933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6331..6342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6451..6467)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6977..7157
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7219..7807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7299..7318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7978..8769,8770..8838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8601..8620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8839..8943)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9018..9037)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9209..9588
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9590..9595,1..193)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
       61 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      121 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      181 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      241 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      301 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      361 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      421 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      481 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      541 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      601 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      661 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      721 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
      781 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
      841 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
      901 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
      961 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1021 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1081 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1141 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1201 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1261 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1321 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1381 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1441 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1501 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1561 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1621 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1681 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1741 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     1801 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     1861 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     1921 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttagtac
     1981 cgggcccgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacag
     2041 acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta
     2101 gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc
     2161 tacattttac atgataggct tggatttcta taagagatac aaatactaaa ttattatttt
     2221 aaaaaacagc acaaaaggaa actcacccta actgtaaagt aattataact tcgtatagta
     2281 taaattatac gaagttataa gccttggttt tttgaattcc gtattaccgc catgcattag
     2341 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     2401 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2461 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     2521 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     2581 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     2641 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2701 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     2761 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2821 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2881 gtgggaggtc tatataagca gagctggttt agtgaaccgt cggatcccag tgtggtggta
     2941 cgggaattca ggaagatggc ggacggcgtg gaccacatag acatttacgc ggatgtcggc
     3001 gaagagttca accaggaagc tgaatatggt gggcatgatc agatagattt gtatgacgat
     3061 gtcatatctc catctgcaaa taatggagat gccccagaag accgagatta catggatact
     3121 ctcccaccaa ctgttggtga tgatgtgggt aaaggagcag caccaaatgt tgtctataca
     3181 tatactggaa agagaattgc attatatatt ggaaatctaa catggtggac aacagatgaa
     3241 gacttaactg aagcagttca ttctttggga gtaaatgata ttttggagat aaaatttttt
     3301 gaaaatcggg caaatggcca gtcaaagggg tttgcccttg ttggtgttgg atctgaagca
     3361 tcttcaaaaa agttaatgga tctgttacct aaaagagaac ttcatggtca gaatcctgtt
     3421 gtaactccat gcaataaaca gttcctgagt caatttgaaa tgcagtccag gaaaactaca
     3481 caatcaggac aaatgtctgg ggaaggtaaa gctggtcctc caggaggcag ttcccgtgca
     3541 gcatttccac aaggtggtag aggacggggc cgttttccag gggctgttcc tggtggggac
     3601 agatttcctg ggccagcagg accaggaggg ccacccccac cttttccagg aaatttgatc
     3661 aagcatcttg ttaaaggaac tcggcctttg ttcctggaaa ctaggattcc atggcatatg
     3721 gggcacagca tagaggaaat acccattttt ggcctaaaag ctggacagac tccaccacgt
     3781 ccacccttag gtcctccagg cccacctggt ccaccaggtc ctccacctcc tggtcaggtt
     3841 ctgcctcctc ctctagctgg gcctcctaat cgaggagatc gccctccacc accagttctt
     3901 tttcctggac aaccttttgg gcagcctcca ttgggtccac ttcctcctgg ccctccacct
     3961 ccagttccag gctacggccc ccctcctggc ccaccacctc cacaacaggg accacctcca
     4021 cctccaggcc cctttccacc tcgtccaccc ggtccacttg ggccacccct tacactagct
     4081 cctcctccgc atcttcctgg accacctcca ggtgccccac cgccagctcc gcatgtgaac
     4141 ccagctttct ttcctccacc aactaacagt ggcatgccta catcagatag ccgaggtcca
     4201 ccaccaacag atccatatgg gcgacctcca ccatatgata ggggtgacta tggcccccct
     4261 ggaagggaaa tggatactgc aagaacgcca ttgagtgaag ctgaatttga agaaatcatg
     4321 aatagaaata gggcaatctc aagcagtgct atttcgagag ctgtgtctga tgccagtgct
     4381 ggtgattatg ggagtgctat tgagacactg gtaactgcaa tttctttaat taaacaatcc
     4441 aaagtatctg ctgatgatcg ttgcaaagtt cttattagtt ctttgcaaga ttgccttcat
     4501 ggaattgagt ccaagtctta tggttctgga tcaagacgtg aacgatcaag agagagggac
     4561 catagtagat cacgagaaaa gagtcgacgt catgaatccg agagtagaga ccgtcatgac
     4621 gattattacg aggaggagag cagagaacga gagaggcacc gggatcgcga ccgagaccgt
     4681 gaccgagagc gtgaccgaga gcgcgaatat cgtcatcgtg aggtaccgcg ggcccgggat
     4741 ccgctagcgc taccggtcgc caccatggcg cgtaaggtcg atctcacctc ctgcgatcgc
     4801 gagccgatcc acatccccgg cagcattcag ccgtgcggct gcctgctagc ctgcgacgcg
     4861 caggcggtgc ggatcacgcg cattacggaa aatgccggcg cgttctttgg acgcgaaact
     4921 ccgcgggtcg gtgagctact cgccgattac ttcggcgaga ccgaagccca tgcgctgcgc
     4981 aacgcactgg cgcagtcctc cgatccaaag cgaccggcgc tgatcttcgg ttggcgcgac
     5041 ggcctgaccg gccgcacctt cgacatctca ctgcatcgcc atgacggtac atcgatcatc
     5101 gagttcgagc ctgcggcggc cgaacaggcc gacaatccgc tgcggctgac gcggcagatc
     5161 atcgcgcgca ccaaagaact gaagtcgctc gaagagatgg ccgcacgggt gccgcgctat
     5221 ctgcaggcga tgctcggcta tcaccgcgtg atgttgtacc gcttcgcgga cgacggctcc
     5281 gggatggtga tcggcgaggc gaagcgcagc gacctcgaga gctttctcgg tcagcacttt
     5341 ccggcgtcgc tggtcccgca gcaggcgcgg ctactgtact tgaagaacgc gatccgcgtg
     5401 gtctcggatt cgcgcggcat cagcagccgg atcgtgcccg agcacgacgc ctccggcgcc
     5461 gcgctcgatc tgtcgttcgc gcacctgcgc agcatctcgc cctgccatct cgaatttctg
     5521 cggaacatgg gcgtcagcgc ctcgatgtcg ctgtcgatca tcattgacgg cacgctatgg
     5581 ggattgatca tctgtcatca ttacgagccg cgtgccgtgc cgatggcgca gcgcgtcgcg
     5641 gccgaaatgt tcgccgactt cttatcgctg cacttcaccg ccgcccacca ccaacgctaa
     5701 gcggccgcat aacttcgtat agtataaatt atacgaagtt ataagccttg ttaacgcgcg
     5761 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagatcatgt ttaagggttc
     5821 cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     5881 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5941 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6001 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6061 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6121 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6181 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6241 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6301 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6361 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6421 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga
     6481 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6541 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     6601 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6661 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     6721 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     6781 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     6841 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     6901 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     6961 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7021 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7081 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7141 gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7621 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7861 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8521 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8881 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8941 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     9001 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     9061 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     9121 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     9181 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     9241 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     9301 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     9361 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     9421 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9481 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9541 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgc
//
