LOCUS       sequence-331092-       19726 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19726)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 19726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU324
FEATURES             Location/Qualifiers
     source          1..19726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8117..8137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13870..13890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    14402..14526
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(14551..14556,14557..14574,14575..14581)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             14635..15315
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(14701..14720)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             15635..15940
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     15819..15838
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(15981..16105)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(18359..18361,18362..18364,18365..19078)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(18404..18425)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(18665..18684)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     19012..19033
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(19346..19363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(19346..19362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19355..19377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    19558..19582
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcggtaccg aataaaagca tttgcgtttg gtttatcatt gcgtttatac aaggacagag
     6781 atccactgag ctggaatagc ttaaaaccat tatcagaaca aaataaacca ttttttgtta
     6841 agaatcagag catagtaaac aacagaaaca acctaagaga ggtaacttgt ccaagaagat
     6901 agctaattat atctatttta taaaagttat catagtttgt aagtcacaaa agatgcaaat
     6961 aacagagaaa ctaggagact tgagaatata cattcttgta tatttgtatt cgagattgtg
     7021 aaaatttgac cataagttta aattcttaaa aagatatatc tgatctaggt gatggttata
     7081 gactgtaatt ttaccacatg tttaatgatg gatagtgaca cacatgacac atcgacaaca
     7141 ctatagcatc ttatttagat tacaacatga aatttttctg taatacatgt ctttgtacat
     7201 aatttaaaag taattcctaa gaaatatatt tatacaagga gtttaaagaa aacatagcat
     7261 aaagttcaat gagtagtaaa aaccatatac agtatatagc ataaagttca atgagtttat
     7321 tacaaaagca ttggttcact ttctgtaaca cgacgttaaa ccttcgtctc caataggagc
     7381 gctactgatt caacatgcca atatatacta aatacgtttc tacagtcaaa tgctttaacg
     7441 tttcatgatt aagtgactat ttaccgtcaa tcctttccca ttcctcccac taatccaact
     7501 ttttaattac tcttaaatca ccactaagct agtaacgcct atcatgaatt agctctacta
     7561 aatctagcaa cctttcaaat ttgcagtatt gcaggtgtct ctgtgtcttt aaaatagttg
     7621 ccttatgatt tcttcggttt caagatgatc aaatagttat agatttcatg ctcacacatg
     7681 ctcattagat gtgtacatac tttacttacc caaatctatt ttctcgcaaa gattttgatg
     7741 gtaaagctga tttggttcta ttgaactaaa tcaaacgagt ttcagactga gtgattctaa
     7801 tccggcccat tagcccctaa acagacccac taattacgca gcttttaata gagtaattac
     7861 acctagttta cccactaaac cactaagcac taattatctc acaatctaat gagcttccct
     7921 cgtaattact tgggctttca ctctaccatt tatttgtaac agtcaagtct ctactgtctc
     7981 tatataaact ctctaaagtt aacacacaat tctcatcaca aacaaatcaa ccaaagcaac
     8041 ttctactctt tcttctttcg accttatcaa tctgttgaga aggtaccaat tcggcgcgcc
     8101 aatggcttct agcccaccga agaagaagcg gaaggtcagc tggaaaatgg acaagaagta
     8161 cagcattgga cttgatattg gtacgaactc agttgggtgg gccgttatca ccgatgaata
     8221 caaggtacct tcgaagaaat ttaaagtgct gggcaacaca gataggcaca gcattaagaa
     8281 gaacttgatc ggagctctgc tctttgactc tggagaaacc gcggaggcga caaggcttaa
     8341 acgtactgcg aggagaaggt acactcgcag gaagaacaga atctgttatc tccaagagat
     8401 ctttagcaac gagatggcga aggtaaggat ttttatgata tagtatgctt atgtattttg
     8461 tactgaaagc atatcctgct tcattgggat attactgaaa gcatttaact acatgtaaac
     8521 tcacttgatg atcaataaac ttgattttgc aggttgacga ctcgttcttc catcgcctcg
     8581 aggaatcctt cctggtagag gaagataaga aacacgagcg tcaccccatc tttgggaata
     8641 ttgttgacga agtagcctat catgaaaagt atccgactat ataccacctt cgcaagaagc
     8701 tggtggactc aaccgataag gcagaccttc ggctcatata cctggctctc gcgcacatga
     8761 taaagtttcg tggccatttc ttgatcgaag gggacctcaa cccggataac tccgatgtgg
     8821 ataaactgtt cattcagctc gtccaaacct acaatcagct gttcgaggag aaccccatca
     8881 atgcatcagg taacattcct tagttacctt tcttttcttt ttccatcata agtttataga
     8941 ttgtacatgc tttgagattt ttctttgcaa acaatctcag gtgtcgacgc caaggcaata
     9001 ctgtctgcca gactttcgaa gtccagacgg cttgagaatc tgatcgctca attgccaggc
     9061 gagaagaaga acggcttgtt cgggaatctg attgcactgt ctctgggcct cacccctaac
     9121 ttcaaaagca actttgacct cgccgaggac gcgaagctgc agctgtcaaa ggatacatac
     9181 gatgatgatc tggacaatct gctcgcccaa ataggtgctc ttgaaattgg aactcttctt
     9241 ttgttgtcta aacctatcaa tttctttgcg gaaatttatt tgaagctgta gagttaaaat
     9301 tgagtctttt aaacttttgt aggtgatcag tatgccgacc tgttcttggc tgccaagaat
     9361 ctgtcagacg ctatcttgct cagtgacatt ctgcgggtca acacggagat aaccaaagcg
     9421 ccacttagcg cctccatgat caagaggtac gacgagcatc accaggatct gacccttctg
     9481 aaggctttgg ttcgccagca actccccgag aagtacaagg agattttctt tgaccaatcg
     9541 aagaatggct acgcagggta cattgatgga ggtaagttgt tacttatgat tgttttcctc
     9601 tctgctacat gtattttgtt gttcatttct gtaagatata agaattgagt tttcctctga
     9661 tgatattatt aggtgcaagt caggaggaat tctacaaatt catcaagcct attctggaaa
     9721 agatggacgg tacagaggag ctgctcgtta aattgaaccg cgaagatttg cttcggaagc
     9781 agcgtacctt cgacaatggc agcataccgc accagatcca cctcggtgag ctgcatgcta
     9841 tcttgaggag gcaagaggac ttctatccgt tcctgaaaga caacagagag aagattgaaa
     9901 agatcctcac gttccgcatt ccctactatg taggttagta tcatatgaag aaatacctag
     9961 tttcagttga tgaatgctat tttctgacct cagttgttct cttttgagaa ttatttcttt
    10021 tctaatttgc ctgatttttc tattaattca ttaggtccac tcgcacgcgg gaactcgcgg
    10081 tttgcgtgga tgacacgcaa atccgaggag actatcacgc cttggaactt cgaagaggtc
    10141 gtggacaagg gtgcgagtgc acagtccttc atcgaaagga tgaccaactt cgataagaat
    10201 ctcccaaatg agaaagtcct gcccaagcat agtctcctgt acgaatactt cacggtctac
    10261 aacgagctga cgaaggtgaa atatgtgacg gaggggatgc gcaaaccggc cttcctgtca
    10321 ggtaaatcct ggtccacact tttacgataa aaacacaaga ttttaaacta tgaactgatc
    10381 aataatcatt cctaaaagac cacacttttg ttttgtttct aaagtaattt ttactgttat
    10441 aacaggtgag cagaagaagg ccattgtcga tctcttgttc aaaaccaatc ggaaggtcac
    10501 tgtgaaacag cttaaagagg actactttaa gaagatcgaa tgctttgatt ctgtggaaat
    10561 cagcggcgtt gaggataggt tcaatgcctc tcttggcaca taccatgacc tgttgaaaat
    10621 catcaaggac aaggacttcc ttgacaacga ggagaacgag gacatcctcg aggacatcgt
    10681 gctgactctc acgctgtttg aggacagaga aatgatcgag gagcgcctta agacttatgc
    10741 gcatctgttc gatgacaagg tcatgaagca gttgaagagg aggagatata caggtaagag
    10801 gtcaaaaggt ttccgcaatg atccctcttt ttttgtttct ctagtttcaa gaatttgggt
    10861 atatgactaa cttctgagtg ttccttgatg catatttgtg atgagacaaa tgtttgttct
    10921 atgttttagg ttggggaagg ctctccagga agctcatcaa cggcatccgc gacaagcaat
    10981 ccggcaagac tatactggac tttctcaaat ccgacggttt tgcgaatcgg aacttcatgc
    11041 agcttattca cgatgactca ctgaccttca aagaagatat ccagaaggcc caagtgtcag
    11101 gtcagggcga tagccttcac gaacacatag ccaacctggc tggatcgcca gctataaaga
    11161 agggcatact gcagacagtg aaggttgtgg atgagctggt gaaggtaagt tctgcatttg
    11221 gttatgctcc ttgcatttta ggtgttcgtc gcacttccat ttccatgaat agctaagatt
    11281 ttttttctct gcattcattc ttcttgcctc agttctaact gtttgtggta tttttgtttt
    11341 aattattgct acaggtcatg ggccgccata agccggagaa catcgtcatc gagatggcga
    11401 gggaaaacca gacgactcag aaagggcaga agaactcacg ggagcgcatg aagcggatag
    11461 aggaaggcat caaggagctt gggagtcaga ttctgaaaga gcacccagtc gaaaatactc
    11521 aactccagaa cgagaagctg tacctctatt acctccagaa tgggagagat atgtacgtcg
    11581 accaagagct cgacattaac agactctccg actatgatgt ggatcacatt gtccctcaat
    11641 ctttcctgaa ggacgatagt attgacaaca aggtaaagca actgtgtttt aatcaatttc
    11701 ttgtcaggat atatggatta taacttaatt tttgagaaat ctgtagtatt tggcgtgaaa
    11761 tgagtttgct ttttggtttc tcccgtgtta taggtcctta cgcgctcaga caagaaccgc
    11821 ggaaaatccg acaatgtacc cagcgaggag gttgtgaaga agatgaagaa ctattggagg
    11881 cagcttttga atgctaagct cataacccaa cggaaattcg acaatctcac gaaggcagaa
    11941 aggggcggac tgtctgagct cgacaaagcc ggcttcatca agcgccagtt ggttgaaact
    12001 cgtcagatta cgaaacatgt ggcccagata ctcgattcgc gtatgaatac gaagtatgat
    12061 gagaatgaca aacttatcag ggaggtaaag gtaaagtttc caactttcct ttaccatatc
    12121 aaactaaagt tcgaaacttt ttatttgatc aacttcaagg ccacccgatc tttctattcc
    12181 tgattaattt gtgatgaatc catattgact tttgatggtt acgcaggtga tcaccctcaa
    12241 gagcaaactg gttagtgact tccggaagga cttccagttt tacaaggttc gcgagatcaa
    12301 caactaccat catgcccatg acgcctacct gaacgccgtt gttggcactg ctctcatcaa
    12361 gaagtatccg aaactggagt ctgagtttgt gtacggggat tacaaggtgt acgacgttag
    12421 gaagatgatc gcgaagtcag aacaagagat cggcaaggct accgcgaaat acttctttta
    12481 ctcgaatatc atgaacttct tcaagacaga gatcactctg gcgaatggtg aaatccggaa
    12541 gaggcctctg atcgagacaa atggcgaaac aggtctgtct ttcctatttc atatgtttaa
    12601 tcctaggaat ttgatcaatt gattgtatgt atgtcgatcc caagactttc ttgttcactt
    12661 atatcttaac tctctctttg ctgtttcttg caggtgagat tgtctgggat aagggcaggg
    12721 attttgcgac tgtgcgtaag gttctcagca tgccccaagt caacatagtc aagaaaacgg
    12781 aggttcaaac cggtggtttc tccaaggagt ccattctccc taagcgcaac tccgacaaac
    12841 tgattgcgag gaagaaggat tgggatccga agaaatacgg aggctttgat agccctaccg
    12901 tggcatacag cgtactggta gtggccaagg tggagaaggg caagagcaag aaactgaaaa
    12961 gcgtcaagga actgcttgga attaccataa tggaaaggtc ctcgttcgag aagaatccga
    13021 tcgacttcct cgaggctaaa ggtaaaatat tggatgccag acgatattct ttcttttgat
    13081 ttgtaacttt ttcctgtcaa ggtcgataaa ttttattttt tttggtaaaa ggtcgataat
    13141 ttttttttgg agccattatg taattttcct aattaactga accaaaatta tacaaaccag
    13201 gttacaaaga ggtgaagaaa gacctcatta tcaaactgcc caagtattcg cttttcgaat
    13261 tggaaaatgg cagaaaacgc atgctggcat ctgccggaga actgcagaag ggcaacgagc
    13321 tggcattgcc cagtaagtac gtcaacttcc tgtacttggc ctcacactat gagaagctga
    13381 aggggtcacc agaggacaac gagcagaagc agttgtttgt cgagcagcac aagcactatc
    13441 ttgatgagat catagagcag atcagcgaat tttccaagcg ggtcattctt gcagacgcta
    13501 acctcgataa ggtaaggact tctcatgaat attagtggca gattagtgtt gttaaagtct
    13561 ttggttagat aatcgatgcc tcctaattgt ccatgtttta ctggttttct acaattaaag
    13621 gtgctttccg cgtacaacaa gcacagagat aagccgataa gggaacaagc ggaaaacatc
    13681 atccacctgt tcacactgac caatctggga gccccagcag cctttaagta cttcgatacc
    13741 actatcgaca gaaagcgcta cacatcaacc aaggaagtgt tggacgctac ccttattcac
    13801 caatctatta cagggctcta tgagacaagg atagatctgt cgcagttggg tggtgactct
    13861 agggctgacc caaagaagaa gcgtaaagtc tgaggcgcgc cgagctccag gcctcccagc
    13921 tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc
    13981 ccacacacca gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct
    14041 atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa
    14101 tgcaaatgga tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat
    14161 tattatctgt tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt
    14221 gatattaagg tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct
    14281 tgaattggtt tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa
    14341 gtaattatgc aatcaagtta agtacagtat aggcttgagc tccctaggcc tgttatccct
    14401 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
    14461 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
    14521 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
    14581 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
    14641 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
    14701 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
    14761 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
    14821 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
    14881 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
    14941 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
    15001 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
    15061 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
    15121 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
    15181 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
    15241 agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag
    15301 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
    15361 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
    15421 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
    15481 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
    15541 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
    15601 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
    15661 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
    15721 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
    15781 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
    15841 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
    15901 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
    15961 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
    16021 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
    16081 gttcagcttt cttgtacaaa gtggttcgat aattccgatc cagcctaggc ccgggcctga
    16141 ggacgcgtcc atggttaatt aagacgtccg gaccgactag tggatcctct agagtcgacc
    16201 tgcaggcatg caagcttctt caagtgtatg taggtatagt aacatgaaca agaacagact
    16261 caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat aatcaaacac
    16321 gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt agccaatagt
    16381 tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag ttcgtctaaa
    16441 caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca gaacacacat
    16501 agagctctct attgattcaa attacgatct gatactgata acgtctagat ttttagggtt
    16561 aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc agaaaacatc
    16621 aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa cctcgttatg
    16681 tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc catggctata
    16741 cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca ctccccagaa
    16801 acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat cgtcgggtcc
    16861 ttgggcgatt gcggcggaag atgggtcagc ttgggcttga agacgagacc cgaatccgag
    16921 tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc gagaggtttg
    16981 agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag agagagaatt
    17041 gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga ggacctgcat
    17101 tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga tttattattt
    17161 gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac taaatcctaa
    17221 atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga atctattgaa
    17281 ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt tcggttcgat
    17341 gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag actcctctcg
    17401 ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa ttgcatgatg
    17461 tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa cactcctacc
    17521 tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct cattctctct
    17581 agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg atatcatcgg
    17641 cagagaccag tacccgatga tgggccgaga ccgagaccag taccagatgt ccggacgagg
    17701 atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca cagctggtgg
    17761 ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt tgactgttgc
    17821 aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca cagttgcact
    17881 cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa ccgttttctc
    17941 ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg caatatgtgc
    18001 atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac aaatgtaaca
    18061 ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt tgacctagct
    18121 agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat gatacctatt
    18181 gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt tggacagtgc
    18241 aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt actacggaca
    18301 gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc acactactat
    18361 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
    18421 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
    18481 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
    18541 cgtgaccacc ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
    18601 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
    18661 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
    18721 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
    18781 gctggagtac aactacttta gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
    18841 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga
    18901 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
    18961 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
    19021 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
    19081 atcttctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
    19141 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
    19201 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
    19261 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
    19321 aatgtttgaa cgatggaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    19381 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    19441 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    19501 gagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
    19561 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    19621 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    19681 ttcccctcgg gatcaaagta ctttaaagta ctttaaagta ctttaa
//
