LOCUS       sequence-331099-       19292 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19292)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 19292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU292
FEATURES             Location/Qualifiers
     source          1..19292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7725..7745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13478..13498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    14010..14134
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(14159..14164,14165..14182,14183..14189)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             14243..14923
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(14309..14328)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             15243..15548
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     15427..15446
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(15589..15713)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             17967..18662
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(18661..18912)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(18922..18939)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(18922..18938)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18931..18953)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    19134..19158
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcaaaaatt acggatatga atataggcat atccgtatcc gaattatccg tttgacagct
     6781 agcaacgatt gtacaattgc ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa
     6841 catcagcgtt aacaaacggc cccgttacgg cccaaacggt catatagagt aacggcgtta
     6901 agcgttgaaa gactcctatc gaaatacgta accgcaaacg tgtcatagtc agatcccctc
     6961 ttccttcacc gcctcaaaca caaaaataat cttctacagc ctatatatac aaccccccct
     7021 tctatctctc ctttctcaca attcatcatc tttctttctc tacccccaat tttaagaaat
     7081 cctctcttct cctcttcatt ttcaaggtaa atctctctct ctctctctct ctctgttatt
     7141 ccttgtttta attaggtatg tattattgct agtttgttaa tctgcttatc ttatgtatgc
     7201 cttatgtgaa tatctttatc ttgttcatct catccgttta gaagctataa atttgttgat
     7261 ttgactgtgt atctacacgt ggttatgttt atatctaatc agatatgaat ttcttcatat
     7321 tgttgcgttt gtgtgtacca atccgaaatc gttgattttt ttcatttaat cgtgtagcta
     7381 attgtacgta tacatatgga tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat
     7441 acagatctga aaacatcact tctctcatct gattgtgttg ttacatacat agatatagat
     7501 ctgttatatc atttttttta ttaattgtgt atatatatat gtgcatagat ctggattaca
     7561 tgattgtgat tatttacatg attttgttat ttacgtatgt atatatgtag atctggactt
     7621 tttggagttg ttgacttgat tgtatttgtg tgtgtatatg tgtgttctga tcttgatatg
     7681 ttatgtatgt gcagcgaatt cggcgcgcca atggcttcta gccaccgaag aagaagcgga
     7741 aggtcagctg gaaaatggac aagaagtaca gcattggact tgatattggt acgaactcag
     7801 ttgggtgggc cgttatcacc gatgaataca aggtaccttc gaagaaattt aaagtgctgg
     7861 gcaacacaga taggcacagc attaagaaga acttgatcgg agctctgctc tttgactctg
     7921 gagaaaccgc ggaggcgaca aggcttaaac gtactgcgag gagaaggtac actcgcagga
     7981 agaacagaat ctgttatctc caagagatct ttagcaacga gatggagaag gtaaggattt
     8041 ttatgatata gtatgcttat gtattttgta ctgaaagcat atcctgcttc attgggatat
     8101 tactgaaagc atttaactac atgtaaactc acttgatgat caataaactt gattttgcag
     8161 gttgacgact cgttcttcca tcgcctcgag gaatccttcc tggtagagga agataagaaa
     8221 cacgagcgtc accccatctt tgggaatatt gttgacgaag tagcctatca tgaaaagtat
     8281 ccgactatat accaccttcg caagaagctg gtggactcaa ccgataaggc agaccttcgg
     8341 ctcatatacc tggctctcgc gcacatgata aagtttcgtg gccatttctt gatcgaaggg
     8401 gacctcaacc cggataactc cgatgtggat aaactgttca ttcagctcgt ccaaacctac
     8461 aatcagctgt tcgaggagaa ccccatcaat gcatcaggta acattcctta gttacctttc
     8521 ttttcttttt ccatcataag tttatagatt gtacatgctt tgagattttt ctttgcaaac
     8581 aatctcaggt gtcgacgcca aggcaatact gtctgccaga ctttcgaagt ccagacggct
     8641 tgagaatctg atcgctcaat tgccaggcga gaagaagaac ggcttgttcg ggaatctgat
     8701 tgcactgtct ctgggcctca cccctaactt caaaagcaac tttgacctcg ccgaggacgc
     8761 gaagctgcag ctgtcaaagg atacatacga tgatgatctg gacaatctgc tcgcccaaat
     8821 aggtgctctt gaaattggaa ctcttctttt gttgtctaaa cctatcaatt tctttgcgga
     8881 aatttatttg aagctgtaga gttaaaattg agtcttttaa acttttgtag gtgatcagta
     8941 tgccgacctg ttcttggctg ccaagaatct gtcagacgct atcttgctca gtgacattct
     9001 gcgggtcaac acggagataa ccaaagcgcc acttagcgcc tccatgatca agaggtacga
     9061 cgagcatcac caggatctga cccttctgaa ggctttggtt cgccagcaac tccccgagaa
     9121 gtacaaggag attttctttg accaatcgaa gaatggctac gcagggtaca ttgatggagg
     9181 taagttgtta cttatgattg ttttcctctc tgctacatgt attttgttgt tcatttctgt
     9241 aagatataag aattgagttt tcctctgatg atattattag gtgcaagtca ggaggaattc
     9301 tacaaattca tcaagcctat tctggaaaag atggacggta cagaggagct gctcgttaaa
     9361 ttgaaccgcg aagatttgct tcggaagcag cgtaccttcg acaatggcag cataccgcac
     9421 cagatccacc tcggtgagct gcatgctatc ttgaggaggc aagaggactt ctatccgttc
     9481 ctgaaagaca acagagagaa gattgaaaag atcctcacgt tccgcattcc ctactatgta
     9541 ggttagtatc atatgaagaa atacctagtt tcagttgatg aatgctattt tctgacctca
     9601 gttgttctct tttgagaatt atttcttttc taatttgcct gatttttcta ttaattcatt
     9661 aggtccactc gcacgcggga actcgcggtt tgcgtggatg acacgcaaat ccgaggagac
     9721 tatcacgcct tggaacttcg aagaggtcgt ggacaagggt gcgagtgcac agtccttcat
     9781 cgaaaggatg accaacttcg ataagaatct cccaaatgag aaagtcctgc ccaagcatag
     9841 tctcctgtac gaatacttca cggtctacaa cgagctgacg aaggtgaaat atgtgacgga
     9901 ggggatgcgc aaaccggcct tcctgtcagg taaatcctgg tccacacttt tacgataaaa
     9961 acacaagatt ttaaactatg aactgatcaa taatcattcc taaaagacca cacttttgtt
    10021 ttgtttctaa agtaattttt actgttataa caggtgagca gaagaaggcc attgtcgatc
    10081 tcttgttcaa aaccaatcgg aaggtcactg tgaaacagct taaagaggac tactttaaga
    10141 agatcgaatg ctttgattct gtggaaatca gcggcgttga ggataggttc aatgcctctc
    10201 ttggcacata ccatgacctg ttgaaaatca tcaaggacaa ggacttcctt gacaacgagg
    10261 agaacgagga catcctcgag gacatcgtgc tgactctcac gctgtttgag gacagagaaa
    10321 tgatcgagga gcgccttaag acttatgcgc atctgttcga tgacaaggtc atgaagcagt
    10381 tgaagaggag gagatataca ggtaagaggt caaaaggttt ccgcaatgat ccctcttttt
    10441 ttgtttctct agtttcaaga atttgggtat atgactaact tctgagtgtt ccttgatgca
    10501 tatttgtgat gagacaaatg tttgttctat gttttaggtt ggggaaggct ctccaggaag
    10561 ctcatcaacg gcatccgcga caagcaatcc ggcaagacta tactggactt tctcaaatcc
    10621 gacggttttg cgaatcggaa cttcatgcag cttattcacg atgactcact gaccttcaaa
    10681 gaagatatcc agaaggccca agtgtcaggt cagggcgata gccttcacga acacatagcc
    10741 aacctggctg gatcgccagc tataaagaag ggcatactgc agacagtgaa ggttgtggat
    10801 gagctggtga aggtaagttc tgcatttggt tatgctcctt gcattttagg tgttcgtcgc
    10861 acttccattt ccatgaatag ctaagatttt ttttctctgc attcattctt cttgcctcag
    10921 ttctaactgt ttgtggtatt tttgttttaa ttattgctac aggtcatggg ccgccataag
    10981 ccggagaaca tcgtcatcga gatggcgagg gaaaaccaga cgactcagaa agggcagaag
    11041 aactcacggg agcgcatgaa gcggatagag gaaggcatca aggagcttgg gagtcagatt
    11101 ctgaaagagc acccagtcga aaatactcaa ctccagaacg agaagctgta cctctattac
    11161 ctccagaatg ggagagatat gtacgtcgac caagagctcg acattaacag actctccgac
    11221 tatgatgtgg atcacattgt ccctcaatct ttcctgaagg acgatagtat tgacaacaag
    11281 gtaaagcaac tgtgttttaa tcaatttctt gtcaggatat atggattata acttaatttt
    11341 tgagaaatct gtagtatttg gcgtgaaatg agtttgcttt ttggtttctc ccgtgttata
    11401 ggtccttacg cgctcagaca agaaccgcgg aaaatccgac aatgtaccca gcgaggaggt
    11461 tgtgaagaag atgaagaact attggaggca gcttttgaat gctaagctca taacccaacg
    11521 gaaattcgac aatctcacga aggcagaaag gggcggactg tctgagctcg acaaagccgg
    11581 cttcatcaag cgccagttgg ttgaaactcg tcagattacg aaacatgtgg cccagatact
    11641 cgattcgcgt atgaatacga agtatgatga gaatgacaaa cttatcaggg aggtaaaggt
    11701 aaagtttcca actttccttt accatatcaa actaaagttc gaaacttttt atttgatcaa
    11761 cttcaaggcc acccgatctt tctattcctg attaatttgt gatgaatcca tattgacttt
    11821 tgatggttac gcaggtgatc accctcaaga gcaaactggt tagtgacttc cggaaggact
    11881 tccagtttta caaggttcgc gagatcaaca actaccatca tgcccatgac gcctacctga
    11941 acgccgttgt tggcactgct ctcatcaaga agtatccgaa actggagtct gagtttgtgt
    12001 acggggatta caaggtgtac gacgttagga agatgatcgc gaagtcagaa caagagatcg
    12061 gcaaggctac cgcgaaatac ttcttttact cgaatatcat gaacttcttc aagacagaga
    12121 tcactctggc gaatggtgaa atccggaaga ggcctctgat cgagacaaat ggcgaaacag
    12181 gtctgtcttt cctatttcat atgtttaatc ctaggaattt gatcaattga ttgtatgtat
    12241 gtcgatccca agactttctt gttcacttat atcttaactc tctctttgct gtttcttgca
    12301 ggtgagattg tctgggataa gggcagggat tttgcgactg tgcgtaaggt tctcagcatg
    12361 ccccaagtca acatagtcaa gaaaacggag gttcaaaccg gtggtttctc caaggagtcc
    12421 attctcccta agcgcaactc cgacaaactg attgcgagga agaaggattg ggatccgaag
    12481 aaatacggag gctttgatag ccctaccgtg gcatacagcg tactggtagt ggccaaggtg
    12541 gagaagggca agagcaagaa actgaaaagc gtcaaggaac tgcttggaat taccataatg
    12601 gaaaggtcct cgttcgagaa gaatccgatc gacttcctcg aggctaaagg taaaatattg
    12661 gatgccagac gatattcttt cttttgattt gtaacttttt cctgtcaagg tcgataaatt
    12721 ttattttttt tggtaaaagg tcgataattt ttttttggag ccattatgta attttcctaa
    12781 ttaactgaac caaaattata caaaccaggt tacaaagagg tgaagaaaga cctcattatc
    12841 aaactgccca agtattcgct tttcgaattg gaaaatggca gaaaacgcat gctggcatct
    12901 gccggagaac tgcagaaggg caacgagctg gcattgccca gtaagtacgt caacttcctg
    12961 tacttggcct cacactatga gaagctgaag gggtcaccag aggacaacga gcagaagcag
    13021 ttgtttgtcg agcagcacaa gcactatctt gatgagatca tagagcagat cagcgaattt
    13081 tccaagcggg tcattcttgc agacgctaac ctcgataagg taaggacttc tcatgaatat
    13141 tagtggcaga ttagtgttgt taaagtcttt ggttagataa tcgatgcctc ctaattgtcc
    13201 atgttttact ggttttctac aattaaaggt gctttccgcg tacaacaagc acagagataa
    13261 gccgataagg gaacaagcgg aaaacatcat ccacctgttc acactgacca atctgggagc
    13321 cccagcagcc tttaagtact tcgataccac tatcgacaga aagcgctaca catcaaccaa
    13381 ggaagtgttg gacgctaccc ttattcacca atctattaca gggctctatg agacaaggat
    13441 agatctgtcg cagttgggtg gtgactctag ggctgaccca aagaagaagc gtaaagtctg
    13501 aggcgcgccg agctccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc
    13561 gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat
    13621 tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc
    13681 tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat
    13741 atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga
    13801 atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac
    13861 agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta
    13921 atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag
    13981 gcttgagctc cctaggcctg ttatccctaa caagtttgta caaaaaagct gaacgagaaa
    14041 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
    14101 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
    14161 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
    14221 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
    14281 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
    14341 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
    14401 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
    14461 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
    14521 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
    14581 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
    14641 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
    14701 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
    14761 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
    14821 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
    14881 tgagtggcag gcggggcgta atctagagga tccggcttac taaaagccag ataacagtat
    14941 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
    15001 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
    15061 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
    15121 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
    15181 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
    15241 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
    15301 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
    15361 gtctgctgtc agataaagtc ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa
    15421 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
    15481 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
    15541 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
    15601 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
    15661 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttcgataa
    15721 ttccgatcca gcctaggccc gggcctgagg acgcgtccat ggttaattaa gacgtccgga
    15781 ccgactagtg gatcctctag agtcgacctg caggcatgca agcttcttca agtgtatgta
    15841 ggtatagtaa catgaacaag aacagactca agtacaagat cgcatacgaa aatggaaatg
    15901 gcaatgtcac ttccacataa tcaaacacga atcctcatat caacaaggcc tgagattcta
    15961 actagctcat aacaacttag ccaatagtta cttgagacta ccaaatgtat gtagaactaa
    16021 agactaaggg acagagagtt cgtctaaaca ggtgaatcta gtcgttgtta tctaataaac
    16081 aattcagccc caaatgcaga acacacatag agctctctat tgattcaaat tacgatctga
    16141 tactgataac gtctagattt ttagggttaa agcaatcaat cacctgacga ttcaaggtgg
    16201 ttggatcatg acgattccag aaaacatcaa gcaagctctc aaagctacac tctttgggat
    16261 catactgaac tctaacaacc tcgttatgtc ccgtagtgcc agtacagaca tcctcgtaac
    16321 tcggattgtg cacgatgcca tggctatacc caacctcggt cttggtcaca ccaggaactc
    16381 tctggtaagc tagctccact ccccagaaac aaccggcgcc aaattgcgcg aattgctgac
    16441 ctgaagacgg aacatcatcg tcgggtcctt gggcgattgc ggcggaagat gggtcagctt
    16501 gggcttgagg acgagacccg aatccgagtc tgttgaaaag gttgttcatt ggggatttgt
    16561 atacggagat tggtcgtcga gaggtttgag ggaaaggaca aatgggtttg gctctggaga
    16621 aagagagtgc ggctttagag agagaattga gaggtttaga gagagatgcg gcggcgatga
    16681 gcggaggaga gacgacgagg acctgcatta tcaaagcagt gacgtggtga aatttggaac
    16741 ttttaagagg cagatagatt tattatttgt atccattttc ttcattgttc tagaatgtcg
    16801 cggaacaaat tttaaaacta aatcctaaat ttttctaatt ttgttgccaa tagtggatat
    16861 gtgggccgta tagaaggaat ctattgaagg cccaaaccca tactgacgag cccaaaggtt
    16921 cgttttgcgt tttatgtttc ggttcgatgc caacgccaca ttctgagcta ggcaaaaaac
    16981 aaacgtgtct ttgaatagac tcctctcgtt aacacatgca gcggctgcat ggtgacgcca
    17041 ttaacacgtg gcctacaatt gcatgatgtc tccattgaca cgtgacttct cgtctccttt
    17101 cttaatatat ctaacaaaca ctcctacctc ttccaaaata tatacacatc tttttgatca
    17161 atctctcatt caaaatctca ttctctctag taaacaagaa caaaaaaatg gcggatacag
    17221 ctagaggaac ccatcacgat atcatcggca gagaccagta cccgatgatg ggccgagacc
    17281 gagaccagta ccagatgtcc ggacgaggat ctgactactc caagtctagg cagattgcta
    17341 aagctgcaac tgctgtcaca gctggtggtt ccctccttgt tctctccagc cttacccttg
    17401 ttggaactgt catagctttg actgttgcaa cacctctgct cgttatcttc agcccaatcc
    17461 ttgtcccggc tctcatcaca gttgcactcc tcatcaccgg ttttctttcc tctggagggt
    17521 ttggcattgc cgctataacc gttttctctt ggatttacaa gtaagcacac atttatcatc
    17581 ttacttcata attttgtgca atatgtgcat gcatgtgttg agccagtagc tttggatcaa
    17641 tttttttggt cgaataacaa atgtaacaat aagaaattgc aaattctagg gaacatttgg
    17701 ttaactaaat acgaaatttg acctagctag cttgaatgtg tctgtgtata tcatctatat
    17761 aggtaaaatg cttggtatga tacctattga ttgtgaatag gtacgcaacg ggagagcacc
    17821 cacagggatc agacaagttg gacagtgcaa ggatgaagtt gggaagcaaa gctcaggatc
    17881 tgaaagacag agctcagtac tacggacagc aacatactgg tggggaacat gaccgtgacc
    17941 gtactcgtgg tggccagcac actactatga gcgagctgat taaggagaac atgcacatga
    18001 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
    18061 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
    18121 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc
    18181 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
    18241 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
    18301 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
    18361 tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
    18421 gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct
    18481 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
    18541 tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
    18601 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaagt
    18661 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
    18721 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
    18781 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
    18841 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
    18901 tgtttgaacg ataagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    18961 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    19021 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgagc
    19081 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
    19141 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
    19201 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
    19261 cctcgggatc aaagtacttt aaagtacttt aa
//
