LOCUS       sequence-331198-        8815 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentivirus for inducible knockdown of human AHR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  TITLE     Halawani et al. bHLH-PAS in neuroregeneration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO-Tet-On-AHR-shRNA1
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..85
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    202..223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(238..243,244..261,262..268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    276..292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     281..303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     300..316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     300..316
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     335..355
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        337..355
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        383..609
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             610..790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    837..962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1455..1688
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1873..1917
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2206..2300
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2217..2236
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2254..2272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2282..2300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2400..2517
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2566..3076
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2995..3013
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3085..3657
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3665..3684
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3712..3730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3741..4361
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3814..3833)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4396..4969
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4563..4580)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4790..4809
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4989..5588
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4989..5008)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5485..5505
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5716..5949
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6021..6142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6058..6077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6112..6131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6182..6317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6338..6356)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6366..6383)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6366..6382)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6375..6397)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6524..6979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6611..6630)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6821..6842
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7005..7109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7110..7178,7179..7970)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7328..7347)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8141..8729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8630..8649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
       61 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      181 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      241 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      301 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      361 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
      421 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
      481 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
      541 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
      601 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      781 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      841 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
      901 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
      961 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1021 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1081 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1141 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     1201 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1261 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     1321 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1381 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1441 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1501 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1561 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1621 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1681 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1741 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     1801 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1861 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     1921 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     1981 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2041 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2101 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2161 ggatctcgac ggtatcgatc acgagactag cctcgagcgg ccgcaatatt tgcatgtcgc
     2221 tatgtgttct gggaaatcac cataaacgtg aaatccctat cagtgataga gacttataag
     2281 ttccctatca gtgatagaga caccgggcaa caagatgagt ctatttactc gagtaaatag
     2341 actcatcttg ttgcttttta attctcgacc tcgagacaaa tggcagtatt catccacaat
     2401 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2461 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2521 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
     2581 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     2641 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     2701 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     2761 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     2821 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     2881 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     2941 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     3001 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     3061 cgacctctct ccccaggggg atctgtgagt ttggggaccc ttgattgttc tttctttttc
     3121 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
     3181 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
     3241 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
     3301 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
     3361 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
     3421 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
     3481 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc
     3541 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
     3601 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     3661 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gtaatacgac
     3721 tcactatagg gcgaattgat atgtctagat tagataaaag taaagtgatt aacagcgcat
     3781 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     3841 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     3901 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     3961 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     4021 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     4081 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     4141 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     4201 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     4261 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     4321 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg
     4381 agttctaggg atctgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     4441 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4501 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4561 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4621 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4681 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4741 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4801 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4861 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4921 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag gatccaccgg
     4981 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5041 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5101 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5161 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5221 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5281 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5341 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5401 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5461 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5521 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5581 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     5641 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5701 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5761 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5941 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6001 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6121 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6301 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6361 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6421 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6541 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6601 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6661 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6721 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6781 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6841 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6901 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6961 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7021 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7081 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7141 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7201 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7261 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7321 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7381 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7441 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7501 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7561 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7621 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7681 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7741 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7801 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7861 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7921 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7981 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8041 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8101 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8161 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8221 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8281 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8341 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8401 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8461 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8521 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8581 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8641 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8701 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8761 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattc
//
