LOCUS       sequence-331199-        8815 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentivirus for inducible knockdown of human AHR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  TITLE     Halawani et al. bHLH-PAS in neuroregeneration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO-Tet-On-AHR-shRNA3
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..68
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    185..206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(221..226,227..244,245..251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    259..275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     264..286
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     318..338
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        320..338
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        366..592
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             593..773
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    820..945
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1438..1671
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1856..1900
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2189..2283
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2200..2219
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2237..2255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2265..2283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2383..2500
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2549..3059
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2978..2996
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3068..3640
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3648..3667
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3695..3713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3724..4344
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3797..3816)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4379..4952
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4546..4563)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4773..4792
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4972..5571
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4972..4991)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5468..5488
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5699..5932
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6004..6125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6041..6060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6095..6114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6165..6300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6321..6339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6349..6366)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6349..6365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6358..6380)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6507..6962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6594..6613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6804..6825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6988..7092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7093..7161,7162..7953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7311..7330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8124..8712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8613..8632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
       61 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      121 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      181 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      241 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      301 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
      361 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
      421 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      481 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      541 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      781 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      841 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      901 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      961 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1021 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1081 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1141 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1201 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1261 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     1321 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1381 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1441 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1501 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1561 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1621 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1681 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1741 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1801 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1861 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1921 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1981 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2041 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2101 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2161 atcacgagac tagcctcgag cggccgcaat atttgcatgt cgctatgtgt tctgggaaat
     2221 caccataaac gtgaaatccc tatcagtgat agagacttat aagttcccta tcagtgatag
     2281 agacaccggc ggcatagaga ccgacttaat ctcgagatta agtcggtctc tatgccgttt
     2341 ttaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2401 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2461 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2521 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
     2581 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     2641 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     2701 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     2761 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     2821 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     2881 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     2941 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     3001 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     3061 gggatctgtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     3121 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     3181 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     3241 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     3301 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     3361 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     3421 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     3481 aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg
     3541 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     3601 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     3661 tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt
     3721 gatatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
     3781 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
     3841 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
     3901 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
     3961 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
     4021 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
     4081 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
     4141 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
     4201 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
     4261 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
     4321 caacttaaat gtgaaagtgg gtccgcgtac agcggctccc gggagttcta gggatctgcc
     4381 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4441 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4501 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4561 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4621 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4681 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     4741 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     4801 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4861 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     4921 acgtggtttt cctttgaaaa acacgatgat aaggatccac cggagcttac catgaccgag
     4981 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5041 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5101 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5161 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5221 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5281 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5341 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5401 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5461 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5521 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     5581 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt acctttaaga
     5641 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5701 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5761 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5821 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5881 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     5941 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6001 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6121 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6181 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6241 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6301 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6361 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6421 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6481 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6541 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6601 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6661 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6721 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6781 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6841 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6901 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     6961 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tatta
//
