LOCUS       sequence-331259-        3709 bp    DNA     circular SYN 15-APR-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-354f-3X
FEATURES             Location/Qualifiers
     source          1..3709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(719..736)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(719..735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(728..750)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(944..963)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1063..1085
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1123..1141)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1209..1313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1314..1382,1383..2174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1532..1551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2345..2933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2834..2853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3087..3104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3221..3242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3257..3262,3263..3280,3281..3287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3295..3311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3300..3322
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3319..3335
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3335
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taccgctgcg tttttttttt catgtgccgg tctcacacgt gcctggagac tagtaagcgc
       61 ttctagtggc ggttaaaacg cggtagacag cgcgtacgtg cgtttaagcg gtgctagagc
      121 tgtctacgac caattgagcg gcctcggcac cgggatgcga tttttttttt catactcgag
      181 catgcaattt tttttttcat gtgccggtct cacacgtgcc tggagactag taagcgcttc
      241 tagtggcggt taaaacgcgg tagacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
      301 ctacgaccaa ttgagcggcc tcggcaccgg gatgcgtttt ttttttcgca gcggtaccgc
      361 tgcgtttttt ttttcatgtg ccggtctcac acgtgcctgg agactagtaa gcgcttctag
      421 tggcggttaa aacgcggtag acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
      481 cgaccaattg agcggcctcg gcaccgggat gcgatttttt ttttcatact cgagcatgca
      541 attttttttt tcatgtgccg gtctcacacg tgcctggaga ctagtaagcg cttctagtgg
      601 cggttaaaac gcggtagaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
      661 ccaattgagc ggcctcggca ccgggatgcg tttttttttt cgcagcggta ccgaattcac
      721 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      781 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      841 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      901 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
      961 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1021 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1081 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1141 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1201 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1381 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1441 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1501 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1561 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1621 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1681 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1741 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1801 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1861 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1921 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1981 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2041 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2101 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2161 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2221 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2881 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3181 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3241 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3301 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttggtaccg
     3361 ctgcgttttt tttttcatgt gccggtctca cacgtgcctg gagactagta agcgcttcta
     3421 gtggcggtta aaacgcggta gacagcgcgt acgtgcgttt aagcggtgct agagctgtct
     3481 acgaccaatt gagcggcctc ggcaccggga tgcgattttt tttttcatac tcgagcatgc
     3541 aatttttttt ttcatgtgcc ggtctcacac gtgcctggag actagtaagc gcttctagtg
     3601 gcggttaaaa cgcggtagac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg
     3661 accaattgag cggcctcggc accgggatgc gttttttttt tcgcagcgg
//
