LOCUS       sequence-331881-        9401 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gal4 activated transgene expression of ultrafast protein calcium 
            sensor under the hsp70 promoter in D. melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Zhang Y, Rozsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, 
            Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC,
            Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, 
            Looger LL
  TITLE     jGCaMP8 Fast Genetically Encoded Calcium Indicators
  JOURNAL   Janelia Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243.
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Janelia 
            Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243."
COMMENT     Sequence Label: pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(175..243,244..1035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(393..412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1206..1794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1695..1714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1881..1950
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2175..2193)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2645..2716,3118..3391,3466..4120,4182..4497,4701..4832,
                     4903..5517)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6547..6641
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6675..6769
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6803..6897
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6931..7025
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7050..7288
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7235..7256
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7303..7369
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7402..7419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(join(9386..9401,1..3))
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttttatag gttaatgtca tgataataat ggtttcttag actcaggtgg cacttttcgg
       61 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      361 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      421 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1321 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1501 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1561 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1621 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1681 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1741 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1801 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttaccg
     1861 tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc
     1921 tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg
     1981 cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg
     2041 ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg
     2101 ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgacaagccg aacatatggg
     2161 cgcgcctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
     2221 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
     2281 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
     2341 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
     2401 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
     2461 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     2521 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     2581 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     2641 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     2701 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     2761 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     2821 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     2881 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     2941 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     3001 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     3061 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     3121 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     3181 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     3241 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     3301 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     3361 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     3421 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     3481 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     3541 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     3601 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     3661 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     3721 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     3781 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     3841 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     3901 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     3961 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     4021 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     4081 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     4141 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     4201 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     4261 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     4321 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     4381 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     4441 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     4501 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     4561 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     4621 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     4681 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     4741 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     4801 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     4861 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     4921 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     4981 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     5041 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     5101 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     5161 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     5221 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     5281 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     5341 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     5401 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     5461 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     5521 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     5581 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     5641 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     5701 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     5761 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     5821 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     5881 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     5941 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     6001 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     6061 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     6121 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     6181 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     6241 agaatacatt taatttagaa aatgcttgga tttcactgga actagggcgc gcctccggaa
     6301 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
     6361 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     6421 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     6481 cctcaacgac aggagcacga tcatgcgcac ccgtggccag ggccgcaagc ttgcatgcct
     6541 gcaggtcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     6601 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagccctagg
     6661 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     6721 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
     6781 gcgctagcgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
     6841 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     6901 agactctagc actagtgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt
     6961 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
     7021 ccgagcggag actctagcga cgtcgagcgc cggagtataa atagaggcgc ttcgtctacg
     7081 gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa
     7141 taaacaagcg cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc
     7201 aattaaaagt aaccagcaac caagtaaatc aactgcaact actgaaatct gccaagaagt
     7261 aattattgaa tacaagaaga gaactctgaa tagatctaaa aggtaggttc aaccactgat
     7321 gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg cggccgcaac
     7381 ttaaaaaaaa aaatcaaaat gcaccaccac catcatcata ccaggcggaa aaagaccttc
     7441 aaagaggtcg ccacagccgt gaaaatcatc ggccgattga tgggattgaa gattaacgtg
     7501 tatattaagg ccgataaaca gaagaacggc atcaaggcca atttccatat acggcacaac
     7561 atcgaagacg gaggcgtcca gctggcctac cattaccagc agaacacacc tatcggcgat
     7621 ggaccggtgc tgttgccgga taatcactac ctgagtgtgg aaagtaagtt gtcgaaggac
     7681 cctaacgaaa aacgcgacca catggtgttg ctcgaattcg tgacggcggc cggaatcaca
     7741 ctgggtatgg acgaactgta caagggcgga accggaggat cgatggtaag caaaggcgag
     7801 gagctgttta ccggcgtggt gccgattttg gtggagttgg acggcgatgt caacggccac
     7861 aagttcagcg tgagcggtga gggtgagggc gatgcaacct acggcaaact gacattgaaa
     7921 ttcatctgca ccacgggaaa actcccggtt ccatggccaa cactggtcac cactctgact
     7981 tatggcgttc agtgctttag ccggtacccc gaccatatga aacagcatga cttctttaag
     8041 tccgcgatgc ccgagggtta catccaagag cgaacaatct tcttcaagga tgacggcaat
     8101 tacaagacgc gcgcagaagt caagttcgag ggcgataccc tggtgaacag gattgaattg
     8161 aaaggcattg actttaagga ggatggaaac atcctgggcc ataagctgga gtacaacctg
     8221 ccggaccagc tgacagagga gcaaatcgcg gagtacaagg aagcctttag cctgttcgac
     8281 aaggacggtg atggcacgat taccacaaaa gagttgggca ccgtcatgcg cagtctggga
     8341 caaaatccta ctgaagctga gctgcaggac atgattaacg aggtcgatgc agatggcgac
     8401 ggcactatcg actttcccga gttcttgaca atgatggccc gcaagatgaa gtaccgggat
     8461 accgaggagg agatccgcga agcctttggc gtgttcgata aagacggcaa tggctacatt
     8521 agcgctgcgg agctgcgcca tgtcatgacc aacttgggcg agaagctcac ggacgaggaa
     8581 gtggacgaaa tgatacgcga agcggacatc gatggcgatg gccaggtcaa ctatgaggaa
     8641 ttcgttcaaa tgatgaccgc aaagtaaggt gtacaaatct agaatgaatc gtttttaaaa
     8701 taacaaatca attgttttat aatattcgta cgattctttg attatgtaat aaaatgtgat
     8761 cattaggaag attacgaaaa atataaaaaa tatgagttct gtgtgtataa caaatgctgt
     8821 aaacgccaca attgtgtttg ttgcaaataa acccatgatt atttgattaa aattgttgtt
     8881 ttctttgttc atagacaata gtgtgttttg cctaaacgtg tactgcataa actccatgcg
     8941 agtgtatagc gagctagtgg ctaacgcttg ccccaccaaa gtagattcgt caaaatcctc
     9001 aatttcatca ccctcctcca agtttaacat ttggccgtcg gaattaactt ctaaagatgc
     9061 cacataatct aataaatgaa atagagattc aaacgtggcg tcatcgtccg tttcgaccat
     9121 ttccgaaaag aactcgggca taaactctat gatttctctg gacgtggtgt tgtcgaaact
     9181 ctcaaagtac gcagtcagga acgtgcgcga catgtcgtcg ggaaactcgc gcggaaacat
     9241 gttgttgtaa ccgaacgggt cccatagcgc caaaaccaaa tctgccagcg tcaatagaat
     9301 gagcacgatg ccgacaatgg agctggcttg gatagcgatt cgagttaacg gccggccgtt
     9361 taaacgaatt cttgaagacg aaagggcctc gtgatacgcc t
//
