LOCUS       sequence-332114-        5502 bp    DNA     circular SYN 15-APR-2025
DEFINITION  This plasmid carries fucO and aldA under the control of P_thrC3 
            promoter, which is used for study about Improving ethylene glycol 
            utilization in Escherichia coli fermentation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Panda S, Fung V, Zhou J, Liang H, Zhou K
  TITLE     Improving ethylene glycol utilization in Escherichia coli 
            fermentation
  JOURNAL   Biochemical Engineering Journal
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochemical
            Engineering Journal"
COMMENT     Sequence Label: pEG02
FEATURES             Location/Qualifiers
     source          1..5502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             346..368
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(3407..4066)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3981..4000
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4067..4169)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4580..4597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4714..5259)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4794..4813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttttcat tctgactgca acgggcaata tgtctctgtg tggattaaaa aaagagtgtc
       61 tgatagcagc ttctgaactg gttacctgcc gtgagtaaat taaaatttta ttgacttagg
      121 tcactaaata ctttaaccaa tataggcata gcgcacagac agataaaaat tacagagtac
      181 acaacatcca tgaaacgcat tagcaccacc attaccacca ccatcaccat taccacaggt
      241 aacggtgcgg gctgacgcgt acaggaaaca cagaaaaaag cccgcacctg acagtgcggg
      301 cttttttttc gaccaaaggt aacgaggtaa caacctagaa ataattttgt ttaactttaa
      361 gaaggagata tacatatggc taacagaatg attctgaacg aaacggcatg gtttggtcgg
      421 ggtgctgttg gggctttaac cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc
      481 gtcaccgata aaacgctggt gcaatgcggc gtggtggcga aagtgaccga taagatggat
      541 gctgcagggc tggcatgggc gatttacgac ggcgtagtgc ccaacccaac aattactgtc
      601 gtcaaagaag ggctcggtgt attccagaat agcggcgcgg attacctgat cgctattggt
      661 ggtggttctc cacaggatac ttgtaaagcg attggcatta tcagcaacaa cccggagttt
      721 gccgatgtgc gtagcctgga agggctttcc ccgaccaata aacccagtgt accgattctg
      781 gcaattccta ccacagcagg tactgcggca gaagtgacca ttaactacgt gatcactgac
      841 gaagagaaac ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt
      901 attgacgctg acatgatgga tggtatgcct ccagcgctga aagctgcgac gggtgtcgat
      961 gcgctcactc atgctattga ggggtatatt acccgtggcg cgtgggcgct aaccgatgca
     1021 ctgcacatta aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat
     1081 aaggatgccg gagaagaaat ggcgctcggg cagtatgttg cgggtatggg cttctcgaat
     1141 gttgggttag ggttggtgca tggtatggcg catccactgg gcgcgtttta taacactcca
     1201 cacggtgttg cgaacgccat cctgttaccg catgtcatgc gttataacgc tgactttacc
     1261 ggtgagaagt accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg
     1321 gaagaggcgc gtaatgccgc tgttgaagcg gtgtttgctc tcaaccgtga tgtcggtatt
     1381 ccgccacatt tgcgtgatgt tggtgtacgc aaggaagaca ttccggcact ggcgcaggcg
     1441 gcactggatg atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta
     1501 gagctttacc ataccgcctg gtaaccgttc atttatcaca aaaggattgt tcgatgtcag
     1561 tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat
     1621 ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg
     1681 ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt
     1741 tgcctgctat tgaacgcgcc agttggttgc gcaaaatctc cgccgggatc cgcgaacgcg
     1801 ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag ctggctgaag
     1861 tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg
     1921 agggcgagat tattcaaagc gatcgtccag gagaaaatat tcttttgttt aaacgtgcgc
     1981 ttggtgtgac taccggcatt ctgccgtgga acttcccgtt cttcctcatt gcccgcaaaa
     2041 tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acctagtgaa tttacgccaa
     2101 acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc ggcgtgttta
     2161 accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg
     2221 caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg actgcggcga
     2281 aaaacatcac caaagtgtgt ctggaattgg ggggtaaagc accagctatc gtaatggacg
     2341 atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc
     2401 aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat cagttcgtca
     2461 atcggctggg tgaagcgatg caggcggttc aatttggtaa ccccgctgaa cgcaacgaca
     2521 ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggtcgagcaa aaagtggcgc
     2581 gcgcagtaga agaaggggcg agagtggcgt tcggtggcaa agcggtagag gggaaaggat
     2641 attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg
     2701 aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat gctatctcaa
     2761 tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg
     2821 cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg
     2881 aagctatgca aggcttccac gccggatggc gtaaatccgg tattggcggc gcagatggta
     2941 aacatggctt gcatgaatat ctgcagaccc aggtggttta tttacagtct tgatgggctg
     3001 aagggtttaa gcgaagacga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     3061 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     3121 gcgcgctgga tgaaaaatta ttagcgttac tggcagagag gcgcgaattc aaacagaaag
     3181 gccatgggct ggcctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
     3241 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg
     3301 accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg
     3361 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     3421 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     3481 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     3541 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     3601 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     3661 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     3721 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     3781 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     3841 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     3901 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     3961 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     4021 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     4081 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     4141 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     4201 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     4261 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     4321 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctttcacaca
     4381 acatagccac gggtgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     4441 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     4501 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     4561 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     4621 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     4681 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     4741 gcatcacgaa atctgacgct caaatcagtg gtgacgaaac ccgacaggac tataaagata
     4801 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     4861 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     4921 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     4981 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     5041 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     5101 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     5161 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     5221 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     5281 ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc
     5341 cccatacgat ataagttgta attctcatgt ttgacagctt atcacccagt cctgctcgct
     5401 tcgctacttg gccataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     5461 atcttcccca tcggtgatgt cttccctcga ctcacacttg gg
//
